Molecularly Imprinted Polymers as Antibody Mimics in Automated On-Line Fluorescent Competitive Assays

Urraca, Javier L.; Moreno‐Bondi, María C.; Orellana, Guillermo; Sellergren, Börje; Hall, Andrew J.

doi:10.1021/ac070277i

Cited by 90 publications

(49 citation statements)

References 21 publications

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“…The first method involves using a labeled template or an analogue derivative in a displacement or competitive assay 577,578 for the fabrication of MIP-based fluorescence sensors. For example, Sellergren et al 579 developed an automated molecularly imprinted sorbent based assay (MIA) based on a fluorescent competitive assay for the rapid and sensitive determination of penicillintype b-lactam antibiotics (BLAs) in biological samples. Generally, the analyte and a constant amount of labeled fluorescent analogue were packed in a reactor in the assay, and then the fluorescence of the labeled derivative was measured which was eluted from the sorbent by using a desorbing solution.…”

Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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a Molecular imprinting technology (MIT), often described as a method of making a molecular lock to match a molecular key, is a technique for the creation of molecularly imprinted polymers (MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Owing to their unique features of structure predictability, recognition specificity and application universality, MIPs have found a wide range of applications in various fields. Herein, we propose to comprehensively review the recent advances in molecular imprinting including versatile perspectives and applications, concerning novel preparation technologies and strategies of MIT, and highlight the applications of MIPs. The fundamentals of MIPs involving essential elements, preparation procedures and characterization methods are briefly outlined.Smart MIT for MIPs is especially highlighted including ingenious MIT (surface imprinting, nanoimprinting, etc.), special strategies of MIT (dummy imprinting, segment imprinting, etc.) and stimuli-responsive MIT (single/dual/ multi-responsive technology). By virtue of smart MIT, new formatted MIPs gain popularity for versatile applications, including sample pretreatment/chromatographic separation (solid phase extraction, monolithic column chromatography, etc.) and chemical/biological sensing (electrochemical sensing, fluorescence sensing, etc.). Finally, we propose the remaining challenges and future perspectives to accelerate the development of MIT, and to utilize it for further developing versatile MIPs with a wide range of applications (650 references).

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Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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“…Due to the specific nature of the interaction between the molecularly imprinted materials and selectable molecules, they have been employed in several analytical techniques, including liquid chromatography, 2 capillary electrophoresis 3 and capillary electrochromatography, 4,5 solid phase extraction, 6 and immunoassay determinations. 7 Molecularly imprinted polymers can be prepared following three different imprinting approaches. 8,9 The non-covalent procedure is the most widely used, because it is relatively simple experimentally and the complexation step during the synthesis is achieved by mixing the template with an appropriate functional monomer, or monomers, Vol.…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted sol-gel silica for solid phase extraction of phenobarbital

Pilau¹,

Silva²,

Jardim³

et al. 2008

J. Braz. Chem. Soc.

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Sílica organicamente modificada e molecularmente impressa foi preparada através de um procedimento sol-gel simples, e avaliada como sorvente específico para extração em fase sólida (Solid Phase Extraction, SPE) de fenobarbital em amostras aquosas e forenses. As propriedades analíticas dessa sílica molecularmente impressa (MIS, molecularly imprinted silica) foram inicialmente avaliadas e o material determinado como específico para as espécies-alvo: o fator de impressão IF, medido como a razão entre o pico do fenobarbital em cromatogramas de MIS e NIS (sílica não-impressa) foi estimado como 58. Este valor é consideravelmente maior que aquele apresentado normalmente para sorventes de impressão convencional baseados em metacrilatos e sugere que interações não-específicas analito/sorvente são insignificantes no MIS. O material foi aplicado no isolamento de fenobarbital de amostras aquosas e plasma; limites de detecção de 10 e 62 µg mL -1 , respectivamente, foram encontrados para essas amostras.A molecularly imprinted organically modified silica was prepared through a simple sol-gel procedure, and evaluated as specific sorbent for solid-phase extraction (SPE) of phenobarbital from aqueous and forensic samples. The analytical properties of the molecularly imprinted silica (MIS, non-imprinted sílica) were initially evaluated and the MIS was found to be specific towards the target species: the imprinting factor IF, measured as the ratio between phenobarbital peak areas in the MIS and NIS chromatograms, was estimated as 58. This value is considerably higher than those usually found for conventional methacrylate-based molecularly imprinted sorbents and suggests that non-specific analyte/sorbent interactions are insignificant in the MIS. This material is applied to the isolation of phenobarbital from aqueous samples and plasma; detection limit of 10 and 62 µg mL -1 was achieved for the former samples, respectively. Keywords: molecularly imprinted silica, sol-gel, ormosils, phenobarbital, SPE IntroductionThere is a demand for sensitive, accurate and simple analytical procedures. One of the tools to achieve these goals is the incorporation of biomolecules and biologicallike processes in the methodologies. However, despite of the specificity achieved on these procedures, natural biomolecules usually are expensive and chemically unstable. Synthetic materials such as molecularly imprinted polymers (MIP) are valid alternatives to these biological matrices. Molecular imprinting was proposed by Pauling 1 as a possible mechanism for the production of antibodies by living systems. According to his model, the synthesis of antibodies was based on the use of the aggressor chemical molecules as templates. Weak intermolecular forces such as hydrogen bonding or van der Waals forces drove proper monomer units to organize themselves around the antigen. Polymerization of the organized monomers and removal of the template would render macromolecules with cavities where the template or other species with similar structure could be highly select...

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“…21 The effect of the imprinted film thickness and polymerization time on the assay sensitivity was also investigated in the present study. Results indicated that when the plate was sealed and irradiated by UV irradiation for 12 h, lower IC 50 values (70 ± 4 mg L -1 ) and optimized competitive binding between MMZ and the HRP conjugate were observed.…”

Section: Condition Optimizationmentioning

confidence: 97%

“…Moreover, the production of antibodies for toxic substances or immunosuppressants is particularly difficult, because they have effects on metabolism and the immune system. 21 Some studies have attempted to design and synthesize artificial antibody to replace antibodies.…”

Section: Introductionmentioning

confidence: 99%

Development of a Biomimetic Enzyme‐linked Immunosorbent Assay Method for the Determination of Methimazole in Urine Sample

Wang

Tang

Fang

et al. 2011

J Chinese Chemical Soc

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A fast and direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method was developed for the determination of methimazole (MMZ) in urine sample based on a molecularly imprinted film as an artificial antibody. This is the first example to monitor methimazole with a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method. The imprinted film was directly synthesized on the well surface of MaxiSorp polystyrene 96-well plate and characterized. The results showed that it exhibited an antibody-like binding ability, rapid adsorption speed, high stability, which was particularly advantageous and suitable for BELISA development. The BELISA method established in this paper had a higher selectivity for MMZ than for the structurally related compounds and the IC 50 (calculated as the concentration giving 50% inhibition of color development) and the detection limit values under optimized experimental conditions were 70 ± 4 mg L -1 and 0.9 ± 0.04 mg L -1 , respectively. The method was applied to the determination of MMZ in spiked urine sample with excellent recoveries ranging from 90% to 95%, and the imprinted film was able to be reused for 20 times without loss of sensitivity. The results obtained by BELISA correlated well with that obtained by the high performance liquid chromatography (HPLC) method.

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Molecularly Imprinted Polymers as Antibody Mimics in Automated On-Line Fluorescent Competitive Assays

Cited by 90 publications

References 21 publications

Molecular imprinting: perspectives and applications

Molecular imprinting: perspectives and applications

Molecularly imprinted sol-gel silica for solid phase extraction of phenobarbital

Development of a Biomimetic Enzyme‐linked Immunosorbent Assay Method for the Determination of Methimazole in Urine Sample

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