Molecularly imprinted PVC beads for the recognition of proteins

Abstract

Ultrathin films of molecularly imprinted polymer (MIP) were prepared by photoiniferter on PVC beads for the selective uptake of lysozyme, taken as a model protein. Acrylamide was selected as the functional monomer and N,N‐methylenebisacrylamide as the crosslinking agent. The copolymerization process was confined to the surface of the PVC beads grafted with diethyldithiocarbamate iniferter initiator in the presence of lysozyme. After extraction of lysozyme from the shell of the PVC‐MIP beads, the latter were then used as artificial receptors for the rebinding of lysozyme. The sequential steps of the modification of PVC beads were monitored by XPS, infrared and Raman spectroscopies. The imprinting step was found to be essential as the PVC‐MIP beads could recognize lysozyme but not the non‐imprinted beads (PVC‐NIP). The binding properties of PVC‐MIP beads were determined using UV spectroscopy from adsorption isotherms of lysozyme, cytochrome, and myoglobin. The imprinted beads were found to be highly selective toward lysozyme over the competitive proteins. This work shows the interest of photoiniferter as an efficient mean for the design of molecularly imprinted polymer beads for rapid, selective removal of proteins. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43694.

A Critical Survey of Dithiocarbamate Reversible Addition‐Fragmentation Chain Transfer (RAFT) Agents in Radical Polymerization

“…[47][48][49] Despite this, iniferter chemistry remains very important in forming photo-initiated grafting from particles or surfaces of various types with a significant number of publications from just 2016-2017. [50][51][52][53][54][55][56][57][58][59][60][61] The process of surface-initiated photoiniferter-mediated polymerization is compared with surface-initiated atom transfer radical polymerization (ATRP), surface reversible RAFT polymerization, and surface-initiated NMP in a recent review. 62 This said, there has been a marked upsurge in interest in photoinitiated RAFT polymerization (mainly using trithiocarbonates or dithioesters) where radicals are generated by direct photolysis of the RAFT agent, [63][64][65][66][67][68][69] or through a photo-redox process involving the RAFT agent, [70][71][72][73] by what can be recognized as Otsu's mechanism.…”

“…Some of the more recent examples include block and star polymer syntheses . Despite this, iniferter chemistry remains very important in forming photo‐initiated grafting from particles or surfaces of various types with a significant number of publications from just 2016–2017 . The process of surface‐initiated photoiniferter‐mediated polymerization is compared with surface‐initiated atom transfer radical polymerization (ATRP), surface reversible RAFT polymerization, and surface‐initiated NMP in a recent review …”

A novel surface molecularly imprinted polymer electrochemical sensor based on porous magnetic TiO2 for highly sensitive and selective detection of tetracycline

“…5C), indicating that the RAFT reagent was successfully grafted onto Fe 3 O 4 @SiO 2 @mTiO 2. 59,60 Fig. 5A-c also shows that the imprinted polymerization layer has signal peaks of O 1s and C 1s, indicating that the MIP has been successfully prepared.…”

Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

“…Zhao et al [9] prepared imprinted membranes using TiO 2 /calcium alginate hydrogel as a matrix to study its adsorption and photocatalytic degradation of methyl orange. Ayadi et al [10] prepared ultrathin films of molecularly imprinted polymer (MIP) for the selective uptake of lysozyme, taken as a model protein. Takeuchi et al [11] prepared lysozyme-imprinted polymers bearing modifiable sites within the imprinted cavity to introduce various functional groups via post-imprinting modifications.…”