Molecularly targeted photothermal ablation improves tumor specificity and immune modulation in a rat model of hepatocellular carcinoma

Muñoz, Nina M.; Dupuis, Crystal; Williams, Madison H.; Dixon, Kathleen; McWatters, Amanda; Avritscher, Rony; Bouchard, Richard R.; Kaseb, Ahmed O.; Schachtschneider, Kyle M.; Rao, Arvind; Sheth, Rahul A.

doi:10.1038/s42003-020-01522-y

Cited by 13 publications

(17 citation statements)

References 54 publications

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“…These results suggested that Fe 3 O 4 and ICG showed a synergistic photothermal effect. Therefore, there was an enhanced photothermal effect of Fe 3 O 4 -ICG@IRM, which can be beneficial for eliminating tumor. − …”

Section: Resultsmentioning

confidence: 99%

Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer

et al. 2021

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Cell-membrane-coated nanoparticles are widely studied due to their inherent cellular properties, such as immune escape and homologous homing. A cell membrane coating can also maintain the relative stability of nanoparticles during circulation in a complex blood environment through cell membrane encapsulation technology. In this study, we fused a murine-derived ID8 ovarian cancer cell membrane with a red blood cell (RBC) membrane to create a hybrid biomimetic coating (IRM), and hybrid IRM camouflaged indocyanine green (ICG)-loaded magnetic nanoparticles (Fe 3 O 4 -ICG@IRM) were fabricated for combination therapy of ovarian cancer. Fe 3 O 4 -ICG@IRM retained both ID8 and RBC cell membrane proteins and exhibited highly specific self-recognition of ID8 cells in vitro and in vivo as well as a prolonged circulation lifetime in blood. Interestingly, in the bilateral flank tumor model, the IRMcoated nanoparticles also activated specific immunity, which killed homologous ID8 tumor cells but had no effect on B16-F10 tumor cells. Furthermore, Fe 3 O 4 -ICG@IRM showed synergistic photothermal therapy, resulting in the release of whole-cell tumor antigens by photothermal-induced tumor necrosis, which further enhanced antitumor immunotherapy for primary tumor and metastatic tumor by activating CD8 + cytotoxic T cells and reducing regulatory Foxp3 + T cells. Together, the biomimetic Fe 3 O 4 -ICG@IRM nanoparticles showed synergistic photothermal-immunotherapy for ovarian cancer.

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Section: Resultsmentioning

confidence: 99%

Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer

et al. 2021

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“…With these key advantages, GeoMx allows researchers to achieve a wide spectrum of spatially resolved transcriptomic and proteomic characterizations of breast cancer samples [4,35,[45][46][47]. Furthermore, the GeoMx spatial profiling is applicable for other tumor types, such as melanoma, lung, prostate, or liver cancers, characterized by high clinical and histological heterogeneity [48][49][50][51][52][53][54][55][56][57][58][59][60].…”

Section: Geomx Digital Spatial Profiler (Dsp) For Spatially Resolved Biologymentioning

confidence: 99%

Best Practices for Spatial Profiling for Breast Cancer Research with the GeoMx® Digital Spatial Profiler

Bergholtz

Carter

Cesano

et al. 2021

Cancers

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Breast cancer is a heterogenous disease with variability in tumor cells and in the surrounding tumor microenvironment (TME). Understanding the molecular diversity in breast cancer is critical for improving prediction of therapeutic response and prognostication. High-plex spatial profiling of tumors enables characterization of heterogeneity in the breast TME, which can holistically illuminate the biology of tumor growth, dissemination and, ultimately, response to therapy. The GeoMx Digital Spatial Profiler (DSP) enables researchers to spatially resolve and quantify proteins and RNA transcripts from tissue sections. The platform is compatible with both formalin-fixed paraffin-embedded and frozen tissues. RNA profiling was developed at the whole transcriptome level for human and mouse samples and protein profiling of 100-plex for human samples. Tissue can be optically segmented for analysis of regions of interest or cell populations to study biology-directed tissue characterization. The GeoMx Breast Cancer Consortium (GBCC) is composed of breast cancer researchers who are developing innovative approaches for spatial profiling to accelerate biomarker discovery. Here, the GBCC presents best practices for GeoMx profiling to promote the collection of high-quality data, optimization of data analysis and integration of datasets to advance collaboration and meta-analyses. Although the capabilities of the platform are presented in the context of breast cancer research, they can be generalized to a variety of other tumor types that are characterized by high heterogeneity.

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“…Moreover, the release of DAMPs to the extracellular space induces an enhanced cytotoxic phenotype of immune cells and an increased infiltration of the tumor microenvironment ( 222 ). Moreover, photothermal ablation modulates the intratumoral myeloid population toward tumor immunogenicity ( 223 ). On the other hand, multiple exposures to radiofrequency radiation hyperthermia suppressed cell-mediated immunocompetence ( 224 ).…”

Section: Physical Methods and Their Mechanismsmentioning

confidence: 99%

Chemotherapy and Physical Therapeutics Modulate Antigens on Cancer Cells

et al. 2022

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Cancer cells possess specific properties, such as multidrug resistance or unlimited proliferation potential, due to the presence of specific proteins on their cell membranes. The release of proliferation-related proteins from the membrane can evoke a loss of adaptive ability in cancer cells and thus enhance the effects of anticancer therapy. The upregulation of cancer-specific membrane antigens results in a better outcome of immunotherapy. Moreover, cytotoxic T-cells may also become more effective when stimulated ex-vivo toward the anticancer response. Therefore, the modulation of membrane proteins may serve as an interesting attempt in anticancer therapy. The presence of membrane antigens relies on various physical factors such as temperature, exposure to radiation, or drugs. Therefore, changing the tumor microenvironment conditions may lead to cancer cells becoming sensitized to subsequent therapy. This paper focuses on the therapeutic approaches modulating membrane antigens and enzymes in anticancer therapy. It aims to analyze the possible methods for modulating the antigens, such as pharmacological treatment, electric field treatment, photodynamic reaction, treatment with magnetic field or X-ray radiation. Besides, an overview of the effects of chemotherapy and immunotherapy on the immunophenotype of cancer cells is presented. Finally, the authors review the clinical trials that involved the modulation of cell immunophenotype in anticancer therapy.

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Molecularly targeted photothermal ablation improves tumor specificity and immune modulation in a rat model of hepatocellular carcinoma

Cited by 13 publications

References 54 publications

Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer

Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer

Best Practices for Spatial Profiling for Breast Cancer Research with the GeoMx® Digital Spatial Profiler

Chemotherapy and Physical Therapeutics Modulate Antigens on Cancer Cells

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