Molekülmassenspektrometrie

Skoog, Douglas A.; Holler, F. James; Crouch, S. R.

doi:10.1007/978-3-642-39726-4_20

Cited by 58 publications

(82 citation statements)

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“…The purity of the sample and the composition of impurities present can be determined from the diffraction pattern, which can be used to determine and correct the lattice parameters of the crystal structure. 53…”

Section: Methodsmentioning

confidence: 99%

Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling

2023

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Section: Methodsmentioning

confidence: 99%

Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling

2023

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“…After R. Luthar proposed the modern form of the Bouguer-Lambert-Beer (BLB) law in 1913 ( Mayerhöfer et al, 2020 ), it has been applied as the essential equation for concentration quantification in various fields such as analytical chemistry, atmospheric physics, microbiology, etc. ( Ingle & Crouch, 1988 ; Perkampus, 2013 ; Skoog et al, 2013 ; Skoog et al, 2017 ; Kirchman & Gasol, 2018 ; Thomas & Burgess, 2022 ). Although it has been referred to as a “law,” it is known that this equation is a limited law ( Perkampus, 2013 ) and is not compatible with Maxwell’s equations ( Mayerhöfer et al, 2016 ; Mayerhöfer et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A novel model extended from the Bouguer-Lambert-Beer law can describe the non-linear absorbance of potassium dichromate solutions and microalgae suspensions

Yeh

Haasdonk

Schmid‐Staiger

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: The Bouguer-Lambert-Beer law is widely used as the fundamental equation for quantification in absorption spectroscopy. However, deviations from the Bouguer-Lambert-Beer law have also been observed, such as chemical deviation and light scattering effect. While it has been proven and shown that the Bouguer-Lambert-Beer law is valid only under very restricted limitations, there are only a few alternatives of analytical models to this law. Based on the observation in the experiments, we propose a novel model to solve the problem of chemical deviation and light scattering effect.Methods: To test the proposed model, a systematic verification was conducted using potassium dichromate solutions and two types of microalgae suspensions with varying concentrations and path lengths.Results: Our proposed model demonstrated excellent performance, with a correlation coefficient (R2) exceeding 0.995 for all tested materials, significantly surpassing the Bouguer-Lambert-Beer law, which had an R2 as low as 0.94. Our results confirm that the absorbance of pure pigment solutions follows the Bouguer-Lambert-Beer law, while the microalgae suspensions do not due to the light scattering effect. We also show that this scattering effect leads to huge deviations for the commonly used linear scaling of the spectra, and we provide a better solution based on the proposed model.Discussion: This work provides a powerful tool for chemical analysis and especially for the quantification of microorganisms, such as the concentration of biomass or intracellular biomolecules. Not only the high accuracy but also the simplicity of the model makes it a practical alternative to the existing Bouguer-Lambert-Beer law.

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“…Also, statin molecules have non-symmetric dipoles and exhibit varying rates of relaxation in solvents with varying polarities. Furthermore, the intermolecular arrangement of the fluorophoric moieties in the excited states of the statin molecules may lead to the formation of hydrogen bonding and cause temporal effects on the fluorescence of statin molecules [ 29 ]. Among the solvents which gave higher fluorescence intensities, water was selected as a solvent in all subsequent experiments because it is not expensive and more importantly is health-safe and fulfills the green approach in the pharmaceutical analysis.…”

Section: Resultsmentioning

confidence: 99%

One-Step Microwell Plate-Based Spectrofluorimetric Assay for Direct Determination of Statins in Bulk Forms and Pharmaceutical Formulations: A Green Eco-Friendly and High-Throughput Analytical Approach

Darwish

Alzoman

et al. 2023

Molecules

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This study describes the development of a one-step microwell spectrofluorimetric assay (MW-SFA) with high sensitivity and throughput for the determination of four statins in their pharmaceutical and formulations (tablets). These statins were pitavastatin (PIT), fluvastatin (FLU), rosuvastatin (ROS) and atorvastatin (ATO). The MW-SFA involves the measurement of the native fluorescence of the statin aqueous solutions. The assay was conducted in white opaque 96-microwell plates, and the fluorescence intensities of the solutions were measured by using a fluorescence microplate reader. The optimum conditions of the assay were established; under which, linear relationships with good correlation coefficients (0.9991–0.9996) were found between the fluorescence intensity and the concentration of the statin drug in a range of 0.2–200 µg mL–1 with limits of detection in a range of 0.1–4.1 µg mL–1. The proposed MW-SFA showed high precision, as the values of the relative standard deviations did not exceed 2.5%. The accuracy of the assay was proven by recovery studies, as the recovery values were 99.5–101.4% (±1.4–2.1%). The assay was applied to the determination of the investigated statins in their tablets. The results were statistically compared with those obtained by a reference method and the results proved to have comparable accuracy and precision of both methods, as evidenced by the t- and F-tests, respectively. The green and eco-friendly feature of the proposed assay was assessed by four different metric tools, and all the results proved that the assay meets the requirements of green and eco-friendly analytical approaches. In addition, ever-increasing miniaturization as handling of large numbers of micro-volume samples simultaneously in the proposed assay gave it a high-throughput feature. Therefore, the assay is a valuable tool for the rapid routine application in the pharmaceutical quality control units for the determination of statins.

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Molekülmassenspektrometrie

Cited by 58 publications

References 0 publications

Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling

Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling

A novel model extended from the Bouguer-Lambert-Beer law can describe the non-linear absorbance of potassium dichromate solutions and microalgae suspensions

One-Step Microwell Plate-Based Spectrofluorimetric Assay for Direct Determination of Statins in Bulk Forms and Pharmaceutical Formulations: A Green Eco-Friendly and High-Throughput Analytical Approach

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Molekülmassenspektrometrie

Cited by 58 publications

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Palladium Schiff base complex-modified Cu(BDC-NH2) metal–organic frameworks for C–N coupling

Palladium Schiff base complex-modified Cu(BDC-NH2) metal–organic frameworks for C–N coupling

A novel model extended from the Bouguer-Lambert-Beer law can describe the non-linear absorbance of potassium dichromate solutions and microalgae suspensions

One-Step Microwell Plate-Based Spectrofluorimetric Assay for Direct Determination of Statins in Bulk Forms and Pharmaceutical Formulations: A Green Eco-Friendly and High-Throughput Analytical Approach

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Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling

Palladium Schiff base complex-modified Cu(BDC-NH₂) metal–organic frameworks for C–N coupling