Monitoring and Source Tracking of Tetracycline Resistance Genes in Lagoons and Groundwater Adjacent to Swine Production Facilities over a 3-Year Period

Koike, Satoshi; Krapac, Ivan G.; Oliver, H. D.; Yannarell, Anthony C.; Chee‐Sanford, Joanne C.; Aminov, Rustam; Mackie, Roderick I.

doi:10.1128/aem.00665-07

Cited by 268 publications

(228 citation statements)

References 36 publications

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“…The gene abundance per sample volume in the APW treatment system (W1, W2, S1, and S2) was significantly higher than those in the OTC production waste (OTC-FR and OTC-ML) (P < 0.05). Since the biomass contents could affect the gene abundance, 12 relative abundance of tet genes and mobile elements (gene copies per 16S rRNA gene) was used for further analyses (Figure 1, SI Figure S2, and SI Table S6). …”

Section: ■ Resultsmentioning

confidence: 99%

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Abundance and Distribution of Tetracycline Resistance Genes and Mobile Elements in an Oxytetracycline Production Wastewater Treatment System

Liu

Zhang

Yang

et al. 2012

Environ. Sci. Technol.

212

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We investigated the abundance and distribution of tetracycline resistance genes (tet genes) in an oxytetracycline (OTC) antibiotic production wastewater (APW) treatment system. Of sixteen tet genes and five mobile elements, nine tet genes (tet(A), tet(C), tet(G), tet(L), tet(M), tet(O), tet(Q), tet(W), and tet(X)) and two mobile elements (class 1 integron (intI1) and transposon Tn916/1545) were successfully quantified by real-time PCR. The relative abundance of tet genes in the effluent and activated sludge (1.2 × 10–4 to 1.3 × 100) of the APW treatment system were up to 2 orders of magnitude higher than those in the OTC fermentation residues (8.5 × 10–5 to 6.7 × 10–3) (P < 0.01), and 1–4 orders of magnitude higher than those in sewage and nonantibiotic production wastewater treatment systems (P < 0.01), showing that the discharge of abundant tet genes from the APW treatment system is worthy of attention. The three most abundant genes in the APW treatment system, tet(A), tet(C), and tet(G), exhibited significant positive correlations with intI1 (R 2 = 0.73, 0.95, and 0.83, respectively; P < 0.05), suggesting that intI1 may be involved in their proliferation. This is the first study showing that some measures may be required to control the discharge of antibiotic resistance genes from treated APW and activated sludge.

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Section: ■ Resultsmentioning

confidence: 99%

“…Tet genes in the environment have been extensively studied in sewage treatment plants (STPs), 11 animal lagoons, 12 and fish farming ponds, 13 where tetracyclines have been frequently detected. Among the 44 known tet genes, more than 29 have been found in the environment.…”

Section: ■ Introductionmentioning

confidence: 99%

Abundance and Distribution of Tetracycline Resistance Genes and Mobile Elements in an Oxytetracycline Production Wastewater Treatment System

Liu

Zhang

Yang

et al. 2012

Environ. Sci. Technol.

212

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“…For 454-pyrosequencing, bacterial 16S rRNA genes in nine sludge samples were amplified with primers 27F (5 0 -AGAGTTT-GATCCTGGCTCAG-3 0 ) and 534R (5 0 -TTACCGCGGCTGCTGGCAC-3 0 ) (Koike et al, 2007). Barcodes that allow sample multiplexing during pyrosequencing were incorporated between the 454 adapter and the forward primers.…”

Section: Pyrosequencing Of Bacterial Communitiesmentioning

confidence: 99%

Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

et al. 2016

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a b s t r a c tThis study aimed to reveal how antibiotic resistance genes (ARGs) and their horizontal and vertical transfer-related items (mobilome and bacterial hosts) respond to the transformation of anaerobic digestion (AD) from mesophilic to thermophilic using one-step temperature increase. The resistomes and mobilomes of mesophilic and thermophilic sludge were investigated using metagenome sequencing, and the changes in 24 representative ARGs belonging to three categories, class 1 integron and bacterial genera during the transition period were further followed using quantitative PCR and 454-pyrosequencing. After the temperature increase, resistome abundance in the digested sludge decreased from 125.97 ppm (day 0, mesophilic) to 50.65 ppm (day 57, thermophilic) with the reduction of most ARG types except for the aminoglycoside resistance genes. Thermophilic sludge also had a smaller mobilome, including plasmids, insertion sequences and integrons, than that of mesophilic sludge, suggesting the lower horizontal transfer potential of ARGs under thermophilic conditions. On the other hand, the total abundance of 18 bacterial genera, which were suggested as the possible hosts for 13 ARGs through network analysis, decreased from 23.27% in mesophilic sludge to 11.92% in thermophilic sludge, indicating fewer hosts for the vertical expansion of ARGs after the increase in temperature. These results indicate that the better reduction of resistome abundance by thermophilic AD might be associated with the decrease of both the horizontal and vertical transferability of ARGs.

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“…Singleplex qPCR using SYBR green detection has previously been used to detect certain antibiotic resistance genes in environmental samples, most frequently identified in water or waste water samples (Volkmann et al, 2004(Volkmann et al, , 2007Borjesson et al, 2009;Knapp et al, 2008;Koike et al, 2007;Pruden et al, 2006;Smith et al, 2004). The SYBR Green RT-PCR chemistry may generate false positive results as it binds to any double-stranded DNA, and as such can also bind to non-specific double-stranded DNA sequences.…”

Section: Discussionmentioning

confidence: 99%

Real-time PCR methods for quantitative monitoring of streptomycin and tetracycline resistance genes in agricultural ecosystems

Walsh

Ingenfeld

Zampicolli

et al. 2011

Journal of Microbiological Methods

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Antibiotic application in plant agriculture is primarily used to control fire blight caused by Erwinia amylovora in pome fruit orchards. In order to facilitate environmental impact assessment for antibiotic applications, we developed and validated culture-independent quantitative real-time PCR multiplex assays for streptomycin (strA, strB, aadA and insertion sequence IS1133) and tetracycline (tetB, tetM and tetW) resistance elements in plant and soil samples. The qPCR were reproducible and consistent whether the DNA was extracted directly from bacteria, plant and soil samples inoculated with bacteria or soil samples prior to and after manure slurry treatment. The genes most frequently identified in soils pre-and post-slurry treatment were strB, aadA, tetB and tetM. All genes tested were detected in soils pre-slurry treatment, and a decrease in relative concentrations of tetB and the streptomycin resistance genes was observed in samples taken post-slurry treatment. These multiplex qPCR assays offer a cost-effective, reliable method for simultaneous quantification of antibiotic resistance genes in complex, environmental sample matrices.

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Monitoring and Source Tracking of Tetracycline Resistance Genes in Lagoons and Groundwater Adjacent to Swine Production Facilities over a 3-Year Period

Cited by 268 publications

References 36 publications

Abundance and Distribution of Tetracycline Resistance Genes and Mobile Elements in an Oxytetracycline Production Wastewater Treatment System

Abundance and Distribution of Tetracycline Resistance Genes and Mobile Elements in an Oxytetracycline Production Wastewater Treatment System

Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

Real-time PCR methods for quantitative monitoring of streptomycin and tetracycline resistance genes in agricultural ecosystems

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