Monitoring biological sulphide oxidation processes using combined respirometric and titrimetric techniques

BACKGROUND: Biological oxidation in biotrickling filters of high H 2 S loads contained in biogas streams still requires further study to reduce elemental sulfur accumulation due to limited gas-liquid oxygen mass transfer inside biotrickling filter beds.Reduction of elemental sulfur accumulation may be improved by regulating the main manipulated variables related to oxygen mass transfer efficiency during biological hydrogen sulfide removal in biotrickling filters. RESULTS: Trickling liquid velocity and co-current flow were selected as the most appropriate manipulated variable and flow pattern configuration compared with manipulating the air supply flow rate and a counter-current flow pattern in order to improve gas-liquid oxygen mass transfer in abiotic conditions. The influence of trickling liquid velocity on the performance of a lab-scale biotrickling filter treating high loads of H 2 S in a biogas mimic and operating in co-current flow at neutral pH and packed with plastic pall rings was investigated.CONCLUSIONS: The effect of trickling liquid velocity modulation between 4.4 and 18.9 m h −1 in biotrickling filter performance was compared with operation without trickling liquid velocity regulation. Velocity regulation resulted in an improvement of 10% in the elimination capacity and, most importantly, a 9% increase in product selectivity to sulfate at a loading rate of 283.8 g S-H 2 S m −3 h −1 . Concentration profiles along the biotrickling filter height evidenced that trickling liquid velocity regulation progressively led to better dissolved oxygen distribution and, thus, enhanced overall biotrickling filter performance.

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“…Biological oxidation reactions of H 2 S by sulfur oxidizing bacteria (SOB) are described by Munz et al using the following catabolic reactions:

H S^{-} + {0.50}_{2} \to S_{0} + O H^{-} ([], Δ G^{0} = prefix- 169 kJ {mol}^{prefix- 1})

S_{0} + {1.50}_{2} + H_{2} O \to prefix S O_{4}^{2 prefix-} + 2 H^{+} ([], Δ G^{0} = prefix- 563.83 kJ {mol}^{prefix- 1})

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Section: Introductionmentioning

confidence: 99%

Influence of trickling liquid velocity and flow pattern in the improvement of oxygen transport in aerobic biotrickling filters for biogas desulfurization

López

Bezerra

Mora

et al. 2015

J of Chemical Tech & Biotech

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“…In particular, respirometry has been demonstrated as a powerful technique to gain insight on kinetics and stoichiometrics of biological processes [3][4][5] since biomass growth and substrate removal are directly associated with the electron acceptor consumption. Moreover, this technique allows obtaining biokinetic characteristics by modeling the respirometric profiles [6] as well as evaluating the inhibitory and/or toxic effects that a particular microbial population may suffer [7][8].…”

Section: Introductionmentioning

confidence: 99%

Investigating the kinetics of autotrophic denitrification with thiosulfate: Modeling the denitritation mechanisms and the effect of the acclimation of SO-NR cultures to nitrite

Mora

Dorado

Gamisans

et al. 2015

Chemical Engineering Journal

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In this work the kinetics of a number of sulfide-oxidizing nitrate-reducing (SO-NR) cultures acclimated and not acclimated to nitrite were characterized. Anoxic respirometry coupled to kinetic modeling of respirometric profiles was the methodology used to study the two-step denitrification associated to thiosulfate oxidation. Autotrophic denitritation was initially studied in a non-acclimated SO-NR culture to confirm that nitrite reduction kinetics could be described through a Haldane-type equation. Afterwards, a kinetic model describing the two-step denitrification (NO 3 -→ NO 2 -→ N 2 ) was calibrated and validated through the estimation of several kinetic parameters from the fitting of experimental respirometric profiles obtained using either nitrate or nitrite as electron acceptors for both acclimated and non-acclimated biomass. The model proposed was a multi-substrate model that considered all the species implicated in the process as well as the stoichiometry associated particularly to the biomass studied in this work. A comparison between the kinetic parameters with the biomass acclimated and nonacclimated to nitrite revealed a 7-fold increase of the Haldane nitrite inhibition constant in the acclimated biomass with respect to the non-acclimated while the nitrite half-saturation constant and the maximum specific growth rate remained almost unchanged. The Fisher Information Matrix method was used to obtain the confidence intervals and also to evaluate the sensitivity and the identifiability in model calibration of each kinetic parameter estimated.

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“…Parameters calibrated were in the range of previously reported values; the latter are presented in Table 3. Munz et al 45 . reported μ max/SOB of 7.4 day −1 (25 °C) for a predominant culture of Halothiobacillaceae family in a membrane reactor working with a solid retention time of 5 days.…”

Section: Resultsmentioning

confidence: 99%

Evaluating and modeling biological sulfur production in the treatment of sulfide‐laden streams containing ammonium

Cueto

Mora

Gabriel

2020

J of Chemical Tech & Biotech

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BACKGROUNDBiological treatment of effluents containing H2S and ammonium are of great interest as both can trigger serious environmental problems when disposed of. The aim of this study was to optimize the production of biosulfur from the partial oxidation of sulfide in sulfide‐ and ammonium‐containing streams. Biological performance was evaluated under various aerating conditions and key kinetic parameters were adjusted based on an existing mathematical model adapted to this system.RESULTSAn optimal conversion of sulfide to S0 of 86% (w/w) was found at an oxidation–reduction potential (ORP) of −380 ± 10 mV and at an O2/S2− molar ratio of 0.44. Partial nitrification was observed at ORP higher than −200 mV and in excess of oxygen supply. Sulfide‐oxidizing bacteria (SOB) outcompeted ammonium‐oxidizing bacteria (AOB) in the competition for dissolved oxygen. In a modeling effort, the maximum specific growth rate for SOB, the sulfur shrinking kinetic constant, the maximum specific growth rate for AOB and the AOB oxygen half‐saturation constant were adjusted to 10.1 day−1, 0.3 mg2/3 VSS mg−2/3 S, 1.75 day−1 and 1.5 mg L−1, respectively, during model calibration.CONCLUSIONSOptimal S0 production was found under limiting O2 conditions in which AOB were not able to outcompete SOB. The mathematical model described satisfactorily the experimental profiles for ammonium, nitrite, sulfide and sulfate as a function of the aeration flow rate. © 2020 Society of Chemical Industry (SCI)

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Monitoring biological sulphide oxidation processes using combined respirometric and titrimetric techniques

Cited by 23 publications

References 24 publications

Influence of trickling liquid velocity and flow pattern in the improvement of oxygen transport in aerobic biotrickling filters for biogas desulfurization

Influence of trickling liquid velocity and flow pattern in the improvement of oxygen transport in aerobic biotrickling filters for biogas desulfurization

Investigating the kinetics of autotrophic denitrification with thiosulfate: Modeling the denitritation mechanisms and the effect of the acclimation of SO-NR cultures to nitrite

Evaluating and modeling biological sulfur production in the treatment of sulfide‐laden streams containing ammonium

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