Monitoring biothiols dynamics in living cells by ratiometric fluorescent gold carbon dots

Xie, Xiaoxue; Peng, Z.-S.; Wang, Ziqi; Hua, Xinyi; Wang, Zhifang; Deng, Keqin; Yang, Xiumei; Huang, Haowen; Liu, Xuanyong

doi:10.1016/j.talanta.2020.121214

Cited by 16 publications

(9 citation statements)

References 52 publications

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“…In general, cancer cells contain more abundant GSH for their high metabolism, whereas less GSH in normal cells. − The introduction of DOX in cells can induce apoptosis, oxidative stress, and decreasing levels of antioxidants . In cells, the decrease of GSH concentration is usually caused by the increase in ROS.…”

Section: Resultsmentioning

confidence: 99%

Glutathione Content Detection of Single Cells under Ingested Doxorubicin by Functionalized Glass Nanopores

Zhang

Wang

et al. 2021

Anal. Chem.

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Glutathione (GSH) level in cells maintains redox homeostasis, so the detection of intracellular GSH is crucial for many pathological processes. In the present study, we developed a cystaminefunctionalized glass nanopore (cG-nanopore) for the specific detection of GSH in single cells through the Michael addition reaction between the amino group of cystamine and polydopamine adhered to the inner wall of the glass nanopore. The cellular GSH will cleave the disulfide bond of cystamine and induce a negative charge increase on the residual surface of the modified nanopore, thus producing a sensitive response on the current−voltage curves. The developed sensing platform showed a good response relationship with the GSH concentration and an excellent selectivity against interfering substances in cells. The variation of GSH content in single HeLa and H8 cells incubated with DOX was then further quantified by this method.

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Section: Resultsmentioning

confidence: 99%

Glutathione Content Detection of Single Cells under Ingested Doxorubicin by Functionalized Glass Nanopores

Zhang

Wang

et al. 2021

Anal. Chem.

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“…GCDs were prepared by a microwave-assisted method. , Fluorescence spectrum shown in Figure a indicates that a strong fluorescence at 435 nm and another weak shoulder peak appears about 570 nm under excitation λ = 380 nm. Transmission electron microscopy (TEM) image of the as-prepared GCDs present in Figure b depicts the size is around 2 nm.…”

Section: Resultsmentioning

confidence: 99%

Real-Time Imaging Redox Status in Biothiols and Ferric Metabolism of Cancer Cells in Ferroptosis Based on Switched Fluorescence Response of Gold Carbon Dots

et al. 2020

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Ferroptosis is an iron-dependent form of regulated cell death. In this study, a ratiometric fluorescent probe, gold carbon dots (GCDs) consisting of carbon skeleton and gold nanoclusters, was used for in situ imaging to monitor redox status in biothiols (glutathione and cysteine) and ferric metabolism of cancer cells in ferroptosis. The as-prepared GCDs can selectively respond to biothiols, interestingly, the fluorescence may be switched to sense ferric ions without interference by biothiols under proper conditions. The robust GCDsprobe exhibits excellent photobleaching resistance and can reversibly respond to intracellular biothiols/ferric ion with high temporal resolution. The 8 h real-time imaging of living cells was employed to track the fluctuation of biothiols, showing the change of redox status in ferroptosis. In addition, release of ferric ions in cells was monitored. The real-time imaging of depletion of biothiols and release of ferric ion in cells indicates the GCDs-probe can monitor how the ferroptosis regulates redox status in biothiols and ferric metabolism.

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“…Based on this "on-off-on" fluorescence response, the use of BN-CDs in assessing biothiols in Hep G2 cells was realized. Except metal ions, metal nanomaterials like silver nanoclusters (AgNCs) [212] and gold nanoclusters [213] can also help CDs to monitor total biothiols in cells by the similar mechanism. Moreover, other types of CDs have been synthesized for intracellular biothiol monitoring without the aid of metal ions, including 2,4-dinitrobenzenesulfonate-functionalized CDs (g-CD-DNBS) [214] and peroxidase-mimetic CDs made from chicken blood.…”

Section: Total Biothiolsmentioning

confidence: 99%

“…Based on this “on–off–on” fluorescence response, the use of BN‐CDs in assessing biothiols in Hep G2 cells was realized. Except metal ions, metal nanomaterials like silver nanoclusters (AgNCs) [ 212 ] and gold nanoclusters [ 213 ] can also help CDs to monitor total biothiols in cells by the similar mechanism.…”

Section: For Detecting Intracellular Compoundsmentioning

confidence: 99%

Carbon Dots for Intracellular Sensing

Lin

Jia

2022

Small Structures

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Carbon dots (CDs), a type of small, carbon element‐based nanomaterials, have found numerous applications in many fields due to their outstanding properties like fascinating photoluminescence characteristic, great biocompatibility, easy and economical synthesis, and facile functionalization. Over the last decade, huge progress and achievements have been made in terms of the applications of CDs in cellular detection. However, a comprehensive review focusing on this topic is still lacking. Herein, the recent progress in CDs‐mediated intracellular detection, including the fluorescence imaging of cellular structures (e.g., nucleus/nucleolus, mitochondrion, lysosome, endoplasmic reticulum, Golgi apparatus, and lipid droplet), the fluorescence detection of a large variety of cellular substances (including endogenous biomacromolecules and their monomers, vitamins, important metabolites, adenosine triphosphate, reactive oxygen species, biothiols, ions, and exogenous compounds), and the monitoring of cellular parameters (pH, temperature, and mitochondrial membrane potential), is thoroughly reviewed. The synthesis, functionalization, and performance of the CDs that serve as fluorescent nanoprobes in living cells are presented. Their underlying working mechanisms are also discussed if available. Finally, the challenges and future research directions of the related research field are listed. This review may foster the future development of more CDs with better cellular sensing performance.

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Monitoring biothiols dynamics in living cells by ratiometric fluorescent gold carbon dots

Cited by 16 publications

References 52 publications

Glutathione Content Detection of Single Cells under Ingested Doxorubicin by Functionalized Glass Nanopores

Glutathione Content Detection of Single Cells under Ingested Doxorubicin by Functionalized Glass Nanopores

Real-Time Imaging Redox Status in Biothiols and Ferric Metabolism of Cancer Cells in Ferroptosis Based on Switched Fluorescence Response of Gold Carbon Dots

Carbon Dots for Intracellular Sensing

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