Monitoring changes in the healthy female metabolome across the menstrual cycle using GC × GC-TOFMS

Eshima, Jarrett; Ong, Stephanie; Davis, Thomas J.; Miranda, Christopher; Krishnamurthy, Devika; Nachtsheim, Abigael C.; Stufken, John; Plaisier, Christopher; Fricks, John; Bean, Heather D.; Smith, Barbara

doi:10.1016/j.jchromb.2019.04.046

Cited by 15 publications

(13 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to this, boys excrete less citrate than girls, at the beginning of puberty, suggesting that citrate excretion could be influenced by sexual hormones [40]. The importance of sexual hormones is also confirmed by data obtained in pre and postmenopausal women [41][42][43].…”

Section: Discussionsupporting

confidence: 60%

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

Caterino

Ruoppolo

Villani

et al. 2020

IJMS

View full text Add to dashboard Cite

The characterization of urinary metabolome, which provides a fingerprint for each individual, is an important step to reach personalized medicine. It is influenced by exogenous and endogenous factors; among them, we investigated sex influences on 72 organic acids measured through GC-MS analysis in the urine of 291 children (152 males; 139 females) aging 1–36 months and stratified in four groups of age. Among the 72 urinary metabolites, in all age groups, 4-hydroxy-butirate and homogentisate are found only in males, whereas 3-hydroxy-dodecanoate, methylcitrate, and phenylacetate are found only in females. Sex differences are still present after age stratification being more numerous during the first 6 months of life. The most relevant sex differences involve the mitochondria homeostasis. In females, citrate cycle, glyoxylate and dicarboxylate metabolism, alanine, aspartate, glutamate, and butanoate metabolism had the highest impact. In males, urinary organic acids were involved in phenylalanine metabolism, citrate cycle, alanine, aspartate and glutamate metabolism, butanoate metabolism, and glyoxylate and dicarboxylate metabolism. In addition, age specifically affected metabolic pathways, the phenylalanine metabolism pathway being affected by age only in males. Relevantly, the age-influenced ranking of metabolic pathways varied in the two sexes. In conclusion, sex deeply influences both quantitatively and qualitatively urinary organic acids levels, the effect of sex being age dependent. Importantly, the sex effects depend on the single organic acid; thus, in some cases the urinary organic acid reference values should be stratified according the sex and age.

show abstract

Section: Discussionsupporting

confidence: 60%

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

Caterino

Ruoppolo

Villani

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…In the context of biofluids, SPME in HS mode is the second most popular technique for volatile analytes characterization by GC×GC [43–46]. Mack et al.…”

Section: Non‐targeted Analysis Applicationsmentioning

confidence: 99%

“…The triphasic fiber is common and appears to be an optimal fiber for biofluid analysis, and has been used to extract VOCs from urine and blood for different objectives such as asthma phenotyping [44], colorectal cancer diagnosis [45], and fertility monitoring [46].…”

Section: Non‐targeted Analysis Applicationsmentioning

confidence: 99%

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Franchina

Zanella

Dubois

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

In this review, we consider and discuss the affinity and complementarity between a generic sample preparation technique and the comprehensive two‐dimensional gas chromatography process. From the initial technical development focus (e.g., on the GC×GC and solid‐phase microextraction techniques), the trend is inevitably shifting toward more applied challenges, and therefore, the preparation of the sample should be carefully considered in any GC×GC separation for an overreaching research. We highlight recent biomedical, food, and plant applications (2016–July 2020), and specifically those in which the combination of tailored sample preparation methods and GC×GC–MS has proven to be beneficial in the challenging aspects of non‐targeted analysis. Specifically on the sample preparation, we report on gas‐phase, solid‐phase, and liquid‐phase extractions, and derivatization procedures that have been used to extract and prepare volatile and semi‐volatile metabolites for the successive GC×GC analysis. Moreover, we also present a milestone section reporting the early works that pioneered the combination of sample preparation techniques with GC×GC for non‐targeted analysis.

show abstract

“…Other studies using samples such as urine, − serum, ,− and tissues targeted the understanding of diet, exercise, or metabolic processes on the body’s health. Cox and co-workers ,, obtained the chemical profile of kidney tissues and different serum samples using GC×GC−TOFMS.…”

Section: Applicationsmentioning

confidence: 99%

Comprehensive Two-Dimensional Gas Chromatography Advances in Technology and Applications: Biennial Update

2019

View full text Add to dashboard Cite

Monitoring changes in the healthy female metabolome across the menstrual cycle using GC × GC-TOFMS

Cited by 15 publications

References 44 publications

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Comprehensive Two-Dimensional Gas Chromatography Advances in Technology and Applications: Biennial Update

Contact Info

Product

Resources

About

Monitoring changes in the healthy female metabolome across the menstrual cycle using GC × GC-TOFMS

Cited by 15 publications

References 44 publications

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

Influence of Sex on Urinary Organic Acids: A Cross-Sectional Study in Children

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Comprehensive Two-Dimensional Gas Chromatography Advances in Technology and Applications: Biennial Update

Contact Info

Product

Resources

About

Monitoring changes in the healthy female metabolome across the menstrual cycle using GC × GC-TOFMS