Monitoring for HHV-6 Infection After Renal Transplantation

Luiz, Claudia R.; Machado, Clarisse Martins; Canto, Cynthia L.M.; Christ, Silvia C.C.; Pestana, José Osmar Medina; Kotton, Camille N.; Camargo, Luís Fernando Aranha

doi:10.1097/tp.0b013e3182807ab7

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…HHV-6 genome was identified by a Nested-PCR assay that is described elsewhere (19). The nested PCR was carried out using the main viral capsid protein primer sets amplifying a 214 bp gene region of the virus genome; (HHV-1 (outer): 5'-CAATGCTTTTCTAGCCGCCTCTTC-3' and HHV-2 (outer): 5'-ACATCTATAATTTTAGACGATCCC-3' and HHV-3 (inner): 5'-TTGTGCGGGTCCGTTCCCATCATA-3 and HHV-4 (inner): 5'-TCGGGATAGAAAAACCTAATCCCT).…”

Section: Hhv6 Screeningmentioning

confidence: 99%

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Aghdam

Nadji

Khoddami

et al. 2020

Arch Pediatr Infect Dis

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Background: Identifying etiologic factors contributing to Wilms tumor (WT) is necessary for its prevention and treatment. Oncogenic viruses cause nearly 20% of all human cancers. Although trials on preventing virus-caused cancers are complex and difficult, but they are not impossible to conduct. Human Cytomegalovirus (HCMV) and human herpes virus-6 (HHV6) can cause different types of cancers. Objectives: The current study aimed to investigate whether HCMV and HHV6-DNA are present in two patients with WT. This is the first study of this kind in Iran. Methods: This is a cross-sectional study performed on patients with kidney disorders who were referring to Mofid Pediatrics Hospital, Tehran (Iran), during 2010-16. In total, 98 kidney samples (49 patients with WT and 49 normal kidneys (autopsy) and kidneys with benign noninfectious lesions) were investigated to identify HCMV and HHV6-DNA. Qualitative Polymerase Chain reaction (PCR) method and nested polymerase chain reaction (nested-PCR) technique were used to identify HCMV and HHV6, respectively. Results: No significant difference was found between WT patients and controls concerning the HCMV or HHV6. Conclusions: Based on the findings, it can be concluded that there is no association between these viruses and WT.

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Section: Hhv6 Screeningmentioning

confidence: 99%

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Aghdam

Nadji

Khoddami

et al. 2020

Arch Pediatr Infect Dis

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“…After quality control of the extracted DNA with the SYBR green real-time PCR (RT-PCR) melting curve for the beta-globin gene (Figure 1), nested PCR was applied to detect the HHV-6 genome in the samples as previously described (20). A 214-bp gene region of the main viral capsid protein was amplified using the nested primer sets ( genome copies of HHV-6 per reaction was determined by the nested PCR assays, using the serial dilutions of AmpliRun ® HHV-6 DNA CONTROL (Vircell, S.L., Granada, Spain).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Human Herpesvirus-6 DNA and Langerhans Cell Histiocytosis

Khoddami

Nadji

Vahdatinia

2016

Arch Pediatr Infect Dis

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Background: Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology that mainly affects young children. The histological feature is the granuloma-like proliferation of Langerhans-type dendritic cells. The possible role of viruses such as Epstein-Barr virus (EBV, HHV-4), human herpesvirus-6 (HHV-6), herpes simplex virus (HSV) types 1 and 2, and cytomegalovirus (CMV, HHV-5) in the pathogenesis of LCH has been suggested in some studies; however, this still remains under debate.

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Impact assessment and investigation of factors associated with herpesviruses viremia in the first year of renal transplantation

Savassi‐Ribas

Almeida

Baez

et al. 2019

Journal of Medical Virology

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The increased risk for opportunistic infections after a renal transplant requires monitoring of viral infections to avoid future complications. Our goal was to investigate the impact and factors associated with Epstein-Barr virus (EBV), human cytomegalovirus (HCMV) and human herpesvirus type 6 (HHV-6) viremia in renal transplant recipients. Whole blood samples were collected monthly from 82 patients during the first semester and then quarterly up to 1 year after transplantation. EBV, HCMV, and HHV-6 were detected and quantified by TaqMan real-time polymerase chain reaction. The results showed that EBV and HCMV viremia were detected in 32 patients (39% each), while HHV-6 viremia in only 3 patients (3.7%). EBV was significantly associated with age (P = .050), thymoglobuline induction (P = .019), mTOR inhibitor-based therapy (P = .003), and female gender (P = .044). HCMV was significantly associated with basiliximab induction (P = .015), mycophenolate mofetil (MMF)-based therapy (P = .003) and allograft acute rejection (P = .033). Moreover, HCMV-disease was correlated with MMF-based therapy (P = .021) and female gender (P = .003). In conclusion, EBV and HCMV viremia were associated with different immunosuppressive induction and maintenance strategies. Additionally, higher HCMV viremia (> 10 4 copies/mL) was related to acute allograft rejection. K E Y W O R D SEpstein-Barr virus, human cytomegalovirus, human herpesvirus type 6, transplant

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Monitoring for HHV-6 Infection After Renal Transplantation

Abstract: Active HHV-6 infection occurs early after renal transplantation and is mostly asymptomatic. Donor or recipient infection may occur at the time of transplantation and are related to higher rates of posttransplantation infections.

Cited by 5 publications

References 30 publications

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Human Herpesvirus-6 DNA and Langerhans Cell Histiocytosis

Impact assessment and investigation of factors associated with herpesviruses viremia in the first year of renal transplantation

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