Monitoring Free Serum IgE In Severe Asthma Patients Treated With Omalizumab

Korn, Stephanie; Haasler, Ina; Fliedner, Florian; Becher, G; Strohner, Pavel; Staatz, Antonia; Taube, Christian; Buhl, Roland

doi:10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a4496

Cited by 8 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sample size is based on a substantially significant change in IgE level observed in the group. Korn et al 29 stated that ELISA can be used to assay free IgE in a concentration range of 1-2,000 IU/mL from peripheral blood samples with a substantially significant change in IgE level due to immunomodulating therapy with 0.5 IU/mL, and the expected standard deviation (SD) of IgE concentration is assumed to be 0.01 IU/mL. 29 For clinical superiority design with a dichotomous variable, the formula for calculating the size (N) is 30 :…”

Section: Sample Size Estimationmentioning

confidence: 99%

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

et al. 2019

View full text Add to dashboard Cite

Background and Objective: There is a pressing need for developing innovative strategies to prevent allergic diseases among children. As house-dust mite (HDM) allergy is often seen in children with gingivitis, strategies should be derived from a conceptual framework of allergen elimination and pathogen eradication; one such strategy is dental scaling and root planing (SRP) to remove dental plaque and periodontal pathogens. The study aimed to evaluate the beneficial effects of comprehensive 6-months dental SRP to reduce the level of immunoglobulin E (IgE) and immunoglobulin G4 (IgG4) in children with gingivitis and HDM allergy. IgE and IgG4, whose production is controlled mainly by Th-2 cells and B cells, are proven biomarkers for atopic inflammatory responses. Methods: The present study conducted a non-blinded randomised controlled trial with superiority design. A total of 10 subjects (age range 6–16 years) with gingivitis and positive skin-prick test to HDM from Pediatric Allergy Outpatient Clinic, Dr. Soetomo General Hospital were enrolled in the present study. Of the 10 subjects, only five received dental SRP. We further evaluated total serum IgE and IgG4 level before and 6 months after treatment. Results and Discussion: Subjects in the standard treatment group showed a slight decrease in the IgE level ([Formula: see text]) but no change in the IgG4 level ([Formula: see text]), while subjects in the intervention group showed a significant decrease in IgE ([Formula: see text]) and IgG4 levels ([Formula: see text]). Conclusion: The study results suggest that 6-month comprehensive dental scaling combined with root planing may help to reduce IgE and IgG4 levels in children with gingivitis and HDM allergy. Furthermore, untreated or undertreated gingivitis is often associated with worsening allergic manifestation and thus should be avoided. Trial Registration: ISRCTN31416107, retrospectively registered on 17 April 2018.

show abstract

Section: Sample Size Estimationmentioning

confidence: 99%

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The sample size is based on a substantial meaningful change in IgE level observed in the group [23]. Korn et al [23] stated that ELISA is able to assay for free IgE in a concentration range of 1-2000 pg/ml from peripheral blood samples with a substantial meaningful change of 0.5 pg/ml and the expected standard deviation of IgE concentration is assumed to be 0.01 pg/ml based on findings by Korn [23]. For this randomized controlled trial design, the formula is:…”

Section: Sample Size Estimationmentioning

confidence: 99%

“…Data were collected by taking venous puncture for measuring total serum IgE and IgG 4 from subjects' blood samples and taking saliva for measuring lipopolysaccharide of Porphyromonas gingivalis concentrations [23]. Total serum IgE and IgG 4 were assessed by direct-sandwich ELISA (R&D System Europe Ltd., Abingdon, UK) according to the manufacturer's protocol.…”

Section: Data Collectionmentioning

confidence: 99%

“…Total serum IgE and IgG 4 concentrations were determined using assay-specific 7-point calibration curves generated with the manufacturer-supplied standard. A value of 0.01 pg/ml was assigned for concentrations below the limit of detection [23].…”

Section: Data Collectionmentioning

confidence: 99%

“…We used a simple sample-processing method for PCR to detect Porphyromonas gingivalis from subjects' saliva [23]. We obtained 500 μL of bacterial culture from salivary secretion, added to 49.5 ml of sterile saline [23].…”

Section: Data Collectionmentioning

confidence: 99%

See 2 more Smart Citations

Correlation between Salivary Lipopolysaccharide ofPorphyromonas gingivaliswith Circulatory Immunoglobulin-E and Immunoglobulin-G₄in Periodontally Healthy Children with House Dust Mite Allergy

Nelwan¹,

Nugraha²,

Endaryanto³

et al. 2019

Gingival Disease - A Professional Approach for Treatment and Prevention

View full text Add to dashboard Cite

The presence of periodontal pathogens in periodontally healthy children is often overlooked or ignored. Since house dust mite allergy often appears among children with chronic gum disease, it is important to understand the role of lipopolysaccharide-a major immunodominant antigen of Porphyromonas gingivalis-in stimulating atopic inflammatory markers of allergies. The aim of the present study is to investigate whether any correlation exists between salivary lipopolysaccharide of Porphyromonas gingivalis with circulatory Immunoglobulin-E and Immunoglobulin-G 4 in periodontally healthy children with house dust mite allergy. We did an analytic observational study between January and December 2017. We recruited 20 periodontally healthy children (aged 6-16 years) from pediatric allergy-immunology clinic at Dr. Soetomo General Hospital (Indonesia). Lipopolysaccharide of Porphyromonas gingivalis was obtained from salivary secretion, while Immunoglobulin-E and Immunoglobulin-G 4 were obtained from venous puncture simultaneously. Immunoglobulin analyses were performed by direct-sandwich ELISA, and lipopolysaccharide analyses were performed by limulus amebocyte lysate (LAL) assay. The average of salivary lipopolysaccharide was 7.21 ± 3.06 μg/ml, Immunoglobulin-E was 114.44 ± 26.19 pg/ml, and Immunoglobulin-G 4 was 31.02 ± 9.09 ng/ml. There was a strong correlation between salivary lipopolysaccharide and Immunoglobulin-E (r 2 = 0.695, p < 0.001), and a very strong correlation between salivary lipopolysaccharide and Immunoglobulin-G 4 (r 2 = 0.796, p < 0.001). Conclusion: data revealed significant correlations between salivary lipopolysaccharide of Porphyromonas gingivalis with circulatory Gingival Disease-A Professional Approach for Treatment and Prevention 2 Immunoglobulin-E and Immunoglobulin-G 4 in periodontally healthy children with house dust mite allergy.

show abstract

Treatable Mechanisms in Asthma

et al. 2021

View full text Add to dashboard Cite

Asthma is a heterogeneous condition, but firm identification of heterogeneity-focused treatments is still lacking. Dividing patients into subgroups of asthma pheno-/endotypes based on combined clinical and cellular biological characteristics and linking them to targeted treatments could be a potentially useful approach to personalize therapy for better outcomes. Nonetheless, there are still many problems related to the identification and validation of asthma phenotypes and endotypes. Alternatively, a precision-medicine strategy for the management of patients with airways disease that is free from the traditional diagnostic labels and based on identifying "treatable traits" in each patient might be preferable. However, it would represent a quite unsophisticated approach because the definition of a treatable trait is too imprecise. In fact, there is still no understanding of the mechanisms underlying treatable traits that allow directing any targeted therapies against any particular treatable trait. Fortunately, in-depth identification of underlying molecular pathways to guide targeted treatment in individual patients is in progress thanks to the improvement in big data management obtained from '-omic' sciences that is greatly increasing knowledge concerning asthma.

show abstract

Monitoring Free Serum IgE In Severe Asthma Patients Treated With Omalizumab

Cited by 8 publications

References 0 publications

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Correlation between Salivary Lipopolysaccharide ofPorphyromonas gingivaliswith Circulatory Immunoglobulin-E and Immunoglobulin-G₄in Periodontally Healthy Children with House Dust Mite Allergy

Treatable Mechanisms in Asthma

Contact Info

Product

Resources

About

Monitoring Free Serum IgE In Severe Asthma Patients Treated With Omalizumab

Cited by 8 publications

References 0 publications

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G4in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G4in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Correlation between Salivary Lipopolysaccharide ofPorphyromonas gingivaliswith Circulatory Immunoglobulin-E and Immunoglobulin-G4in Periodontally Healthy Children with House Dust Mite Allergy

Treatable Mechanisms in Asthma

Contact Info

Product

Resources

About

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

Correlation between Salivary Lipopolysaccharide ofPorphyromonas gingivaliswith Circulatory Immunoglobulin-E and Immunoglobulin-G₄in Periodontally Healthy Children with House Dust Mite Allergy