Monitoring gestational diabetes at the point-of-care <i>via</i> dual glycated albumin lateral flow assays in conjunction with a handheld reader

Belsare, Sayali; Tseng, Derek; Ozcan, Aydogan; Coté, Gerard L.

doi:10.1039/d2an01238c

Cited by 7 publications

(4 citation statements)

References 58 publications

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“…For an example of the sandwich format, an LFA strategy tracked both glycated albumin and albumin concentrations in serum to record the glycemic status of patients with gestational diabetes mellitus (GDM), a glucose intolerance disorder in pregnant people [55]. The assay was able to detect albumin as a target molecule between AuNPs conjugated with both detection aptamer and capture aptamer on the test line.…”

Section: Paper-based Colorimetric Aptasensorsmentioning

confidence: 99%

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

et al. 2023

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Recent innovations in point-of-care (POC) diagnostic technologies have paved a critical road for the improved application of biomedicine through the deployment of accurate and affordable programs into resource-scarce settings. The utilization of antibodies as a bio-recognition element in POC devices is currently limited due to obstacles associated with cost and production, impeding its widespread adoption. One promising alternative, on the other hand, is aptamer integration, i.e., short sequences of single-stranded DNA and RNA structures. The advantageous properties of these molecules are as follows: small molecular size, amenability to chemical modification, low- or nonimmunogenic characteristics, and their reproducibility within a short generation time. The utilization of these aforementioned features is critical in developing sensitive and portable POC systems. Furthermore, the deficiencies related to past experimental efforts to improve biosensor schematics, including the design of biorecognition elements, can be tackled with the integration of computational tools. These complementary tools enable the prediction of the reliability and functionality of the molecular structure of aptamers. In this review, we have overviewed the usage of aptamers in the development of novel and portable POC devices, in addition to highlighting the insights that simulations and other computational methods can provide into the use of aptamer modeling for POC integration.

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Section: Paper-based Colorimetric Aptasensorsmentioning

confidence: 99%

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

et al. 2023

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“…In a study published by Belsare et al [145], two aptamer-based lateral flow assays were developed to measure GHSA and serum albumin in their relevant concentration ranges [145]. Herein, both assays used AuNP-based colorimetry, and the dynamic ranges for both assays were 3 to 20 mg/mL and 20 to 50 mg/mL, respectively, which are both physiologically relevant.…”

Section: Aptasensors For Metabolic Biomarkersmentioning

confidence: 99%

“…Herein, both assays used AuNP-based colorimetry, and the dynamic ranges for both assays were 3 to 20 mg/mL and 20 to 50 mg/mL, respectively, which are both physiologically relevant. The LOD was calculated to be 0.8 mg/mL and 1.5 mg/mL, respectively [145].…”

Section: Aptasensors For Metabolic Biomarkersmentioning

confidence: 99%

Nucleic Acid Aptamer-Based Biosensors: A Review

Sequeira-Antunes,

Ferreira

2023

Biomedicines

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Aptamers, short strands of either DNA, RNA, or peptides, known for their exceptional specificity and high binding affinity to target molecules, are providing significant advancements in the field of health. When seamlessly integrated into biosensor platforms, aptamers give rise to aptasensors, unlocking a new dimension in point-of-care diagnostics with rapid response times and remarkable versatility. As such, this review aims to present an overview of the distinct advantages conferred by aptamers over traditional antibodies as the molecular recognition element in biosensors. Additionally, it delves into the realm of specific aptamers made for the detection of biomarkers associated with infectious diseases, cancer, cardiovascular diseases, and metabolomic and neurological disorders. The review further elucidates the varying binding assays and transducer techniques that support the development of aptasensors. Ultimately, this review discusses the current state of point-of-care diagnostics facilitated by aptasensors and underscores the immense potential of these technologies in advancing the landscape of healthcare delivery.

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“…Utilising a polyaryl film and poly (ethylene glycol) coating on a gold electrode, the system produces significant signals of GA against interfering proteins, successfully detecting GA concentrations of 0.1, 1, and 10 mg/mL, demonstrating its utility as a platform for enzyme-/antibody-free protein analysis [ 46 ]. Additionally, Gerard et al [ 47 ] proposed a point-of-care (POC) system for monitoring gestational diabetes mellitus (GDM) by combining lateral flow assays (LFAs) with a handheld colorimetric reader, targeting glycated albumin (GA) as an intermediate interval biomarker. The system presents two aptamer-based assays, one for GA with a dynamic range of 3–20 mg/ml, another for serum albumin with a range of 20–50 ml/ml, and successfully integrates both into a dual assay cartridge and hence demonstrating potential as an effective platform for tracking GDM at the POC.…”

Section: Introductionmentioning

confidence: 99%

A selective LSPR biosensor for molecular-level glycated albumin detection

Mondal,

Hossain,

Birbilis

2023

Heliyon

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Monitoring gestational diabetes at the point-of-care via dual glycated albumin lateral flow assays in conjunction with a handheld reader

Abstract: A dual assay cartridge was developed and used in conjunction with a handheld reader for sensing % glycated albumin to monitor gestational diabetes at home.

Cited by 7 publications

References 58 publications

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

Nucleic Acid Aptamer-Based Biosensors: A Review

A selective LSPR biosensor for molecular-level glycated albumin detection

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