Monitoring Intact Viruses Using Aptamers

Kumar, Penmetcha K. R.

doi:10.3390/bios6030040

Cited by 31 publications

(25 citation statements)

References 78 publications

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“…In many cases, aptamers possessing neutralizing activity have been regarded as important components of antiviral strategies 27 , 29 , 32 and have been clinically used to treat viral infection 14 , 33 . In this study aptamer IBRV-A4 had a neutralization titre of 1:55 and was shown to efficiently inhibit multiplication of BoHV-1 in MDBK cells as shown by plaque reduction assay (Fig.…”

Section: Discussionmentioning

confidence: 99%

A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry

Zhang

Zhou

et al. 2017

Sci Rep

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Bovine herpesvirus 1 (BoHV-1) is an important pathogen of domestic and wild cattle responsible for major economic losses in dairy and beef industries throughout the world. Inhibition of viral entry plays a crucial role in the control of BoHV-1 infection and aptamers have been reported to inhibit viral replication. In this study, nine DNA aptamers that target BoHV-1 were generated using systemic evolution of ligands by exponential enrichment. Of the nine candidates, aptamer IBRV-A4 exhibited the highest affinity and specificity for BoHV-1, which bound to BoHV-1 with a Kd value of 3.519 nM and demonstrated the greatest virus binding as shown by fluorescence imaging. The neutralizing ability of aptamer IBRV-A4 was determined using neutralization assays and real time PCR in BoHV-1 infected Madin-darby bovine kidney cells. Virus titration, immunofluorescence and confocal laser scanning microscopy showed virus replication significantly decreased when aptamer IBRV-A4 was added to BoHV-1 infected MDBK cells at 0 and 0.5 hours post-infection, whereas no change was seen when IBRV-A4 was added 2 hours post-infection. This concludes that aptamer IBRV-A4 efficiently inhibits viral entry of BoHV-1 in MDBK cells and is therefore a novel tool for diagnosis and treatment of BoHV-1 infection in cattle.

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Section: Discussionmentioning

confidence: 99%

A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry

Zhang

Zhou

et al. 2017

Sci Rep

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“…Aptamers are also much more stable than antibodies making them suitable in applications requiring harsh conditions, (e.g., high temperature or extreme pH) [143]. General review articles about aptamer-based sensors [144] and aptamer-based sensors for viruses are available in literature [145][146][147].…”

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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“…Their lower molecular weight enhances the stability to sustain repetitious denaturation and renaturation. Aptasensor, due to its advantages, is increasingly explored, particularly in intact virus detection using SPR and other platforms 127 .…”

Section: Other Advanced Technologies In Poc Of Foodborne Virusesmentioning

confidence: 99%

Recent Advances in Biosensor Development for Foodborne Virus Detection

Neethirajan¹,

Ahmed²,

Chand³

et al. 2017

Nanotheranostics

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Outbreaks of foodborne diseases related to fresh produce have been increasing in North America and Europe. Viral foodborne pathogens are poorly understood, suffering from insufficient awareness and surveillance due to the limits on knowledge, availability, and costs of related technologies and devices. Current foodborne viruses are emphasized and newly emerging foodborne viruses are beginning to attract interest. To face current challenges regarding foodborne pathogens, a point-of-care (POC) concept has been introduced to food testing technology and device. POC device development involves technologies such as microfluidics, nanomaterials, biosensors and other advanced techniques. These advanced technologies, together with the challenges in developing foodborne virus detection assays and devices, are described and analysed in this critical review. Advanced technologies provide a path forward for foodborne virus detection, but more research and development will be needed to provide the level of manufacturing capacity required.

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Monitoring Intact Viruses Using Aptamers

Cited by 31 publications

References 78 publications

A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry

A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Recent Advances in Biosensor Development for Foodborne Virus Detection

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