Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes

Iaccino, Enrico; Mimmi, Selena; Dattilo, Vincenzo; Marino, Fabiola; Candeloro, Patrizio; Loria, Antonio Di; Marimpietri, Danilo; Pisano, Antonio; Albano, Fabio; Vecchio, Eleonora; Ceglia, Simona; Golino, Gaetanina; Lupia, Antonio; Fiume, Giuseppe; Quinto, Ileana; Scala, Giuseppe

doi:10.1186/s12943-017-0730-8

Cited by 56 publications

(50 citation statements)

References 26 publications

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“…Exosomes were purified from serum of MS patients and/or healthy donors and physically characterized as previously demonstrated (29). Analysis with Zetasizer Nano ZS90 (Malvern Instruments, Malvern, United Kingdom) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Exosome‐associated miRNA profile as a prognostic tool for therapy response monitoring in multiple sclerosis patients

et al. 2018

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Multiple sclerosis (MS) is an autoimmune pathology leading to neurodegeneration. Because of the complexity and heterogenic etiology of this disease, diagnosis and treatment for individual patients are challenging. Exosome-associated microRNAs (miRNAs) have recently emerged as a new class of diagnostic biomarkers involved in both autoimmune and neurologic disorders. Interesting new evidence has emerged showing that circulating miRNAs are dysregulated in MS body fluids, including serum, plasma, and cerebrospinal fluid. We hypothesized that exosome-associated miRNAs could present a readily accessible blood-based assay for MS disease. We detected expression of miRNAs by quantitative PCR on a small cohort of MS patients. We analyzed circulating exosome-associated miRNAs of MS patients before and after therapy and found that 14 exosome-associated miRNAs were significantly down-regulated, while 2 exosome-associated miRNAs were significantly up-regulated in IFN-β-treated relapsing-remitting MS patients with response to therapy compared to those without response. We identified a serum miRNA panel that could be used to monitor the response to IFN-β therapy. Overall, these data suggest that circulating exosome-associated miRNA profiling could represent an easily detectable biomarker of disease and treatment response.-Manna, I., Iaccino, E., Dattilo, V., Barone, S., Vecchio, E., Mimmi, S., Filippelli, E., Demonte, G., Polidoro, S., Granata, A., Scannapieco, S., Quinto, I., Valentino, P., Quattrone, A. Exosome-associated miRNA profile as a prognostic tool for therapy response monitoring in multiple sclerosis patients.

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Section: Resultsmentioning

confidence: 99%

Exosome‐associated miRNA profile as a prognostic tool for therapy response monitoring in multiple sclerosis patients

et al. 2018

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“…Exosomes were extracted from the serum using a polymer-based kit, ExoQuick ® (System Biosciences Mountain View, Palo Alto, CA, USA) according to a previous study [17]. Exosomes were analyzed by flow cytometry (FC) and characterized by transmission electron microscopy (TEM).…”

Section: Exosomes Isolation and Mirna Detectionmentioning

confidence: 99%

“…They act on mRNA primarily as inhibitors (translational repression or degradation) affecting several physiological processes [13]. While in circulation, serum miRNAs are highly degradable, however, when transported in microvesicles (exosomes) these molecules are more stable and can serve as reliable diagnostic biomarkers in diseased patients [16][17][18]. Exosomes being small extracellular mycelial vesicles [19] protect RNA from RNAse degradation [20].…”

Section: Introductionmentioning

confidence: 99%

Expression of Serum Exosomal miRNA 122 and Lipoprotein Levels in Dogs Naturally Infected by Leishmania infantum: A Preliminary Study

Loria

Dattilo

Santoro

et al. 2020

Animals

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Current knowledge on the role of exosomal microRNA (miRNA) in canine leishmaniasis (CL), with particular regards to the interaction between miR-122 and lipid alterations, is limited. The aim of this study was to isolate/characterize exosomes in canine serum and evaluate the expression of miR-122 in ten healthy and ten leishmaniotic dogs. Serum exosomes were isolated using a polymer-based kit, ExoQuick® and characterized by flow cytometry and transmission electron microscopy, whereas miR-122-5p expression was evaluated by quantitative reverse-transcriptase polymerase chain reaction. A significant decreased expression of exosomal miR-122-5p, decreased serum levels of high-density lipoproteins, and increased serum levels of low-density lipoproteins were seen in leishmaniotic dogs when compared with healthy dogs. These results suggest that hepatic dysfunctions induced by the parasite interfere with lipoprotein status. The decreased expression of exosomal miR122 represents an additional effect of Leishmania infection in dogs as in people.

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“…For example, VEGF-dependent pathways have been considered a target of tumor therapy [39]. However, while some cancer types have shown an effective therapeutic response, the benefits of anti-angiogenic agents have been revealed to be transient and followed by tumor relapse [39][40][41]. With IBTK being required for cancer B cell survival [21,22] and lymphoma vascularization (this study), the combination of IBTK-targeting therapies with anti-angiogenic agents could improve the therapeutic efficacy.…”

Section: Discussionmentioning

confidence: 94%

IBTK Haploinsufficiency Affects the Tumor Microenvironment of Myc-Driven Lymphoma in E-myc Mice

Vecchio

Fiume

Mignogna

et al. 2020

IJMS

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The tumor microenvironment is a dynamic and interactive supporting network of various components, including blood vessels, cytokines, chemokines, and immune cells, which sustain the tumor cell’s survival and growth. Murine models of lymphoma are useful to study tumor biology, the microenvironment, and mechanisms of response to therapy. Lymphomas are heterogeneous hematologic malignancies, and the complex microenvironment from which they arise and their multifaceted genetic basis represents a challenge for the generation and use of an appropriate murine model. So, it is important to choose the correct methodology. Recently, we supported the first evidence on the pro-oncogenic action of IBTK in Myc-driven B cell lymphomagenesis in mice, inhibiting apoptosis in the pre-cancerous stage. We used the transgenic Eμ-myc mouse model of non-Hodgkin’s lymphoma and Ibtk hemizygous mice to evaluate the tumor development of Myc-driven lymphoma. Here, we report that the allelic loss of Ibtk alters the immunophenotype of Myc-driven B cell lymphomas, increasing the rate of pre-B cells and affecting the tumor microenvironment in Eμ-myc mice. In particular, we observed enhanced tumor angiogenesis, increasing pro-angiogenic and lymphangiogenic factors, such as VEGF, MMP-9, CCL2, and VEGFD, and a significant recruitment of tumor-associated macrophages in lymphomas of Ibtk+/- Eμ-myc compared to Ibtk+/+ Eμ-myc mice. In summary, these results indicate that IBTK haploinsufficiency promotes Myc tumor development by modifying the tumor microenvironment.

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Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes

Cited by 56 publications

References 26 publications

Exosome‐associated miRNA profile as a prognostic tool for therapy response monitoring in multiple sclerosis patients

Exosome‐associated miRNA profile as a prognostic tool for therapy response monitoring in multiple sclerosis patients

Expression of Serum Exosomal miRNA 122 and Lipoprotein Levels in Dogs Naturally Infected by Leishmania infantum: A Preliminary Study

IBTK Haploinsufficiency Affects the Tumor Microenvironment of Myc-Driven Lymphoma in E-myc Mice

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