Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection

Capillary electrophoresis of proteins 1999-2001

“…In addition to UV, LIF, and MS detection, some other less common and novel detection methods have been explored, such as amperometric detection of myoglobin in human urine [84]. The use of chemiluminescence detection for CIEF has been demonstrated with a fiberoptic based detection cell [85], yielding a LOD in the nM range and a linear range of two orders of magnitude.…”

Detecção eletroquímica em eletroforese capilar

“…Sistemas mais sofisticados envolvendo a remoção 40,52,157 , enzimas e proteínas [160][161][162] , co-fatores 163 , nucleotídeos e similares [164][165][166] , ácidos policarboxílicos 167 , hormônios 168 , bem como outros metabólitos, como ácido úrico 169 [197][198][199][200][201] . A ED também é bastante apropriada em separações eletroforéticas em meio não-aquoso (NACE), uma vez que a maioria dos solventes utilizados absorve fortemente na região do UV, o que dificulta as medidas fotométricas.…”

Amperometric and voltammetric detection for capillary electrophoresis

“…Peptides or proteins containing electroactive amino acid residues such as tyrosine, cysteine, or tryptophan or other redox active functional groups have been detected at carbon fiber electrodes [47,50,51] and a cuprous oxide-modified carbon composite electrode [17]. Gold/mercury electrodes are often employed to detect glutathione [52,53]; the PQQ-mediated detection of glutathione has also been reported [18].…”

“…Cysteine [18], myoglobin [50], and acyclovir [83] have been determined in urine, and pyruvate has been detected in sweat [89]. CEEC was also used to quantify 8-hydroxydeoxyguanosine, an indicator of the degree of oxidative DNA damage [76].…”