Monitoring neutrophils and platelets during casein‐induced anaphylaxis in an experimental <scp>BALB</scp>/c mouse model

Krishnamurthy, Durga; Starkl, Philipp; Szalai, Krisztina; Roth‐Walter, Franziska; Diesner, Susanne C.; Mittlböck, Martina; Mannhalter, Christine; Untersmayr, Eva; Jensen‐Jarolim, Erika

doi:10.1111/j.1365-2222.2012.04012.x

Cited by 14 publications

(13 citation statements)

References 38 publications

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“…The drop of leukocytes upon allergen challenge in the mouse study is in accordance with a previous observation in anaphylaxis, [31] but opposed the results from the human allergic subjects in our study, where peripheral leukocyte counts significantly increased after allergen challenge. A subset analysis showed that human segmented neutrophils and to some extent basophils accounted for the total leukocyte elevation, without relevant changes in less matured neutrophils or eosinophils.…”

Section: Discussionsupporting

confidence: 90%

Peripheral Erythrocytes Decrease upon Specific Respiratory Challenge with Grass Pollen Allergen in Sensitized Mice and in Human Subjects

et al. 2014

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Background and AimsSpecific hyper-responsiveness towards an allergen and non-specific airway hyperreactivity both impair quality of life in patients with respiratory allergic diseases. We aimed to investigate cellular responses following specific and non-specific airway challenges locally and systemically in i) sensitized BALB/c mice challenged with grass pollen allergen Phl p 5, and in ii) grass pollen sensitized allergic rhinitis subjects undergoing specific airway challenge in the Vienna Challenge Chamber (VCC).Methods and ResultsBALB/c mice (n = 20) were intraperitoneally immunized with grass pollen allergen Phl p 5 and afterwards aerosol challenged with either the specific allergen Phl p 5 (n = 10) or the non-specific antigen ovalbumin (OVA) (n = 10). A protocol for inducing allergic asthma as well as allergic rhinitis, according to the united airway concept, was used. Both groups of exposed mice showed significantly reduced physical activity after airway challenge. Specific airway challenge further resulted in goblet cell hyperplasia, enhanced mucous secretion, intrapulmonary leukocyte infiltration and lymphoid follicle formation, associated with significant expression of IL-4, IL-5 and IL-13 in splenocytes and also partially in lung tissue. Concerning circulating blood cell dynamics, we observed a significant drop of erythrocyte counts, hemoglobin and hematocrit levels in both mouse groups, challenged with allergen or OVA. A significant decrease in circulating erythrocytes and hematocrit levels after airway challenges with grass pollen allergen was also found in grass pollen sensitized human rhinitis subjects (n = 42) at the VCC. The effects on peripheral leukocyte counts in mice and humans however were opposed, possibly due to the different primary inflammation sites.ConclusionOur data revealed that, besides significant leukocyte dynamics, particularly erythrocytes are involved in acute hypersensitivity reactions to respiratory allergens. A rapid recruitment of erythrocytes to the lungs to compensate for hypoxia is a possible explanation for these findings.

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Section: Discussionsupporting

confidence: 90%

Peripheral Erythrocytes Decrease upon Specific Respiratory Challenge with Grass Pollen Allergen in Sensitized Mice and in Human Subjects

et al. 2014

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“…Murine studies have also studied platelets and erythrocytes response during anaphylaxis and have suggested possible participation via an allergic mechanism 18, 19. Specifically platelet's had a significant drop during anaphylaxis in the murine study by Krishnamurthy et al 18.…”

Section: Discussionmentioning

confidence: 99%

A case series: Association of anaphylaxis with a significant decrease in platelet levels and possible secondary risk of thrombosis

Peppers

Vatsayan

Dalal

et al. 2018

Immunity Inflam & Disease

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IntroductionAnaphylaxis is a life threatening systemic inflammatory process that share mediators involved in the coagulation cascade. Platelet activating factor, known to increase platelet aggregation, has also been implicated as an important mediator of anaphylaxis. Although other inflammatory reactions are associated with an increased risk of thrombosis, anaphylaxis is currently not reported as one of them. Furthermore the role platelets may have in the perianaphylaxis period is not well understood. We here in present a retrospective case series of three patients that had platelet aberrations suggestive of PAF involvement and clinically significant thrombosis in close relationship with anaphylaxis.ObjectiveTo investigate platelet response before and after anaphylaxis and indirect observation evidence of platelet activating factors involvement with possible increased risk of thrombosis.MethodsA retrospective investigation into medical records including medication administrations times, laboratory, and radiology results. Platelet levels pre‐ and post‐ anaphylaxis were statistically analyzed.ResultsCase 1, a 44 year old man had an anaphylactic reaction shortly after envenomation and subsequently suffered an acute infarction with thrombus in a cerebral artery. Case 2 is a 49 year old man with idiopathic anaphylaxis who developed a deep vein thrombosis after a protracted anaphylaxis event. Case 3 involved an 18 year old female with acute myeloid leukemia was found to have a thrombus in the celiac trunk following anaphylaxis. A paired two‐tailed Wilcoxon test on the subjects pre and post anaphylactic platelet levels resulted in a overall P < 0.0001.Conclusions and Clinical RelevanceThese three cases illustrate the potential role platelets may have in anaphylaxis and possible increased secondary risk for the development of thrombosis. Larger studies are required to determine incidence and risk factors for blood clots following anaphylaxis in order to provide management or screening recommendations.

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“…To our knowledge, the role of neutrophils in the liver, following nanomaterial exposure has not been addressed previously, however, it has been shown that nautrophils can accumulate within hepatic microvasculature, following increase in inflammatory mediators such as TNF-α, IL1, MIP-2 and PAF [23,24]. Furthermore, in a recent study, an increase in numbers of neutrophils in the liver of BALB/c mice was observed during anaphylaxis [25].…”

Section: Resultsmentioning

confidence: 98%

Engineered Nanomaterial Impact in the Liver following Exposure via an Intravenous Route–The Role of Polymorphonuclear Leukocytes and Gene Expression in the Organ

Kermanizadeh¹,

Brown²,

Hutchison

et al. 2012

J Nanomedic Nanotechnol

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Monitoring neutrophils and platelets during casein‐induced anaphylaxis in an experimental BALB/c mouse model

Cited by 14 publications

References 38 publications

Peripheral Erythrocytes Decrease upon Specific Respiratory Challenge with Grass Pollen Allergen in Sensitized Mice and in Human Subjects

Peripheral Erythrocytes Decrease upon Specific Respiratory Challenge with Grass Pollen Allergen in Sensitized Mice and in Human Subjects

A case series: Association of anaphylaxis with a significant decrease in platelet levels and possible secondary risk of thrombosis

Engineered Nanomaterial Impact in the Liver following Exposure via an Intravenous Route–The Role of Polymorphonuclear Leukocytes and Gene Expression in the Organ

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