Monitoring of microRNA using molecular beacons approaches: Recent advances

Bidar, Negar; Oroojalian, Fatemeh; Baradaran, Behzad; Eyvazi, Shirin; Jebelli, Asiyeh; Hosseini, Seyed Samad; Pashazadeh-Panahi, Paria; Mokhtarzadeh, Ahad; Guárdia, Miguel de la

doi:10.1016/j.trac.2020.116021

Cited by 30 publications

(27 citation statements)

References 116 publications

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“…Furthermore, miRNA-21, miRNA-27, and miRNA-155 were overexpressed in breast cancer. In addition, the miRNA expression level can not only detect tumor occurrence but also serve as an indicator for evaluating prognosis and efficacy. , …”

Section: Rnas: Function Detection and Imagingmentioning

confidence: 99%

“…MBs consist of two parts: target-binding loop and stem region. The target-binding loop is the target recognition region, usually composed of 25–35 bases, which can be identified and hybridized by target sequences. , The stem region composes two complementary short arm sequences, which make the probe form a closed loop, and is usually composed of 5–8 bases. The fluorophore dyes and quencher attached at two ends of the stem are usually used to establish signal transduction in MBs. , …”

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

“…Moreover, visualization of RNA can be realized, which is very valuable compare with traditional detection methods. , MBs have developed rapidly in just a few years and have been become the most-studied probes for RNA imaging. According to the structure and function, MBs are generally classified into common MBs, aptamers, nuclease resistance modified MBs, and DNAzyme. , …”

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

“…to effectively deliver them to the treatment site. − On the other hand, due to their unique properties, NPs can also be used for tumor treatment, such as photothermal therapy (PTT), photodynamic therapy, hemodynamic therapy, immunotherapy, and sonodynamic therapy, etc. − In addition, NPs can also be used to remodel the tumor microenvironment (TME), such as consuming GSH, generating oxygen, and reshaping the immune tumor. , Furthermore, NPs can be used for tumor imaging, such as photoacoustic (PA) imaging, computed tomography (CT) imaging, NIR imaging, and photothermal imaging, etc . With the continuous deepening of nanotechnology, it has been found that some nanomaterials have superior fluorescence quenching ability (nanoquencher), such as gold nanoparticles (Au NPs), polydopamine (PDA), graphene oxide (GO), molybdenum disulfide (MoS 2 ) nanosheet (NS), etc., and have been used to construct biosensors . There are many nanosystems (Nano-MBs) based on MBs and nanoquenchers constructed for tumor detection and treatment and have gradually become a research hotspot.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

Yang

Song

Wang

et al. 2022

ACS Appl. Nano Mater.

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As one of the two nucleic acids used by extant biochemistry, RNA is of great importance for cellular function and development, and its expression can affect cell homeostasis. Therefore, accurate detection of RNA is the key contributor to disease diagnosis, including tumor detection. Compared with conventional RNA detection methods, molecular beacons (MBs) have high selectivity for target molecules detection, which have been widely used in RNA detection. However, the background fluorescence of typical MBs will seriously affect the detection sensitivity and accuracy. In addition, the poor affinity and weak resistance to the nuclease digestion of MBs also limit its applications. With the development of nanotechnology, many nanomaterials with fluorescence quenching ability have been widely used as quenchers and carriers for MBs to construct nanoprobes (Nano-MBs). In this review, we summarize the recent developments of Nano-MBs in RNA detection and imaging over the past decade. First, the structure, working principle, and classification of MBs are discussed. Then, we introduce several typical Nano-MBs based on gold nanoparticles, polydopamine, graphene oxide, molybdenum disulfide, and quantum dots. Particular emphasis is placed on the application of Nano-MBs in mRNA and miRNA detection and imaging in the diagnosis and treatment of diseases, as well as in cancer. Finally, the current challenges and future perspectives are also discussed.

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Section: Rnas: Function Detection and Imagingmentioning

confidence: 99%

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

Yang

Song

Wang

et al. 2022

ACS Appl. Nano Mater.

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“…The fluorescent labeling technique is one of the methods of visualizing miRNAs or short interfering RNA (siRNA) working in the RNAi system of cells. Although fluorescent in situ hybridization or molecular beacon-based analyses employing fluorescently labeled complementary antisense can detect the target miRNA [ 17 , 18 , 19 ], it would inhibit the function of the target RNA. Therefore, the development of the direct labeling of miRNA or siRNA with fluorophores has been considered.…”

Section: Introductionmentioning

confidence: 99%

Development and Modification of Pre-miRNAs with a FRET Dye Pair for the Intracellular Visualization of Processing Intermediates That Are Generated in Cells

Kamimoto

Zhu

Asanuma

2021

Sensors

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microRNAs (miRNAs) are small non-coding ribonucleic acids (RNAs), which regulate gene expression via the RNA interference (RNAi) system. miRNAs have attracted enormous interest because of their biological significance and disease relationship. In cell systems, the generation of miRNA is regulated by multiple steps: the transfer of primary miRNA from the nucleus to the cytosol, the generation of the precursor-miRNA (pre-miRNA), the production of double-stranded RNA from pre-miRNA by the Dicer, the interaction with protein argonaute-2 (AGO2), and the subsequent release of one strand to form miRISC with AGO2. In this study, we attempt to visualize the intermediates that were generated in the miRNA-maturation step in the cells to acquire a detailed understanding of the maturation process of miRNA. To achieve this, we developed pre-miRNAs labeling with a Dicer- or AGO2-responsible fluorescence resonance energy transfer (FRET) dye pair. We observed that modifications with the dye at suitable positions did not interfere with the biological activities of pre-miRNAs. Further, imaging analyses employing these pre-miRNAs demonstrated that the processing of pre-miRNA promoted the accumulation of miRNA at the specific foci in the cytosol. The FRET-labeled pre-miRNA would further elucidate the mechanisms of the RNAi process and provide the basis for development of nucleic acid drugs working in the RNAi system.

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A comprehensive overview on the crosstalk between microRNAs and viral pathogenesis and infection

Bahojb Mahdavi,

Jebelli,

Aghbash

et al. 2024

Medicinal Research Reviews

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Infections caused by viruses as the smallest infectious agents, pose a major threat to global public health. Viral infections utilize different host mechanisms to facilitate their own propagation and pathogenesis. MicroRNAs (miRNAs), as small noncoding RNA molecules, play important regulatory roles in different diseases, including viral infections. They can promote or inhibit viral infection and have a pro‐viral or antiviral role. Also, viral infections can modulate the expression of host miRNAs. Furthermore, viruses from different families evade the host immune response by producing their own miRNAs called viral miRNAs (v‐miRNAs). Understanding the replication cycle of viruses and their relation with host miRNAs and v‐miRNAs can help to find new treatments against viral infections. In this review, we aim to outline the structure, genome, and replication cycle of various viruses including hepatitis B, hepatitis C, influenza A virus, coronavirus, human immunodeficiency virus, human papillomavirus, herpes simplex virus, Epstein–Barr virus, Dengue virus, Zika virus, and Ebola virus. We also discuss the role of different host miRNAs and v‐miRNAs and their role in the pathogenesis of these viral infections.

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Monitoring of microRNA using molecular beacons approaches: Recent advances

Cited by 30 publications

References 116 publications

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

Development and Modification of Pre-miRNAs with a FRET Dye Pair for the Intracellular Visualization of Processing Intermediates That Are Generated in Cells

A comprehensive overview on the crosstalk between microRNAs and viral pathogenesis and infection

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