Monitoring of monocyte functional state after extracorporeal circulation: A flow cytometry study

Abstract

Results: Cardiac surgery with CPB induced down-modulation of surface molecules expression on peripheral monocytes, especially at 24 h after CPB, for CD18, CD11a, and CD11b (P < 0.003) and for the CD15 adhesive cluster (P ‫؍‬ 0.0028) and HLA-DR (P < 0.001). At 4 h after CPB, downregulation was observed for CD14 (P ‫؍‬ 0.004), CD45 (P ‫؍‬ 0.014), and CD15 (P ‫؍‬ 0.0056). A loss of MPO was detected in venous peripheral (at 24 h after CPB, P ‫؍‬ 0.01) or coronary (at reperfusion, P < 0.02) blood. The CD15 cluster complex exhibited a down-modulation in coronary blood (at reperfusion, P ‫؍‬ 0.0003). Spontaneous intracellular production of IL-1␤, IL-6, and IL-8 decreased at 24 h after CPB (P < 0.05).Conclusions: The down-modulation of integrins and adhesive receptor expression and the loss of MPO suggest a strong activation and shedding reaction of circulating monocyte after CPB, further exacerbated by contact with coronary ischemic vessels. The changes of differentiation antigens may reflect the appearance of a partially immature population immediately after CPB. The reduced proinflammatory cytokine production, observed at 24 h after CPB, suggests a functional polarization of circulating monocytes.

Pro-inflammatory cytokines after different kinds of cardio-thoracic surgical procedures: is what we see what we know?

“…Due to different findings, it remained unclear whether the myocardium or the lungs were the main source of IL-8 secretion. Our data indicate that the latter hypothesis is unlikely and that an activation of immunocompetent cells synthesizing IL-8 during ECC-as shown by Sbrana et al [13,14] and Naldini et al-is unlikely to be the cause of elevated IL-8 levels following CPB CAB.…”

An increased platelet–leukocytes interaction at the culprit site of coronary artery occlusion in acute myocardial infarction: A pathogenic role for “no-reflow” phenomenon?

“…Blood samples anti-coagulated with 3.8% trisodium-citrate were stained as previously reported [19,20]. The following combinations of PerCP-, FITC-, and PE-conjugated monoclonal antibodies were employed: (a) CD14/CD18/CD11b; (b) CD14/HLADR/CD49e; (c) CD14/CD66b/CD29.…”

“…Monoclonal antibodies were obtained from Becton Dickinson, Pharmingen and Immunotech (Beckman Coulter, Fullerton, CA, USA). The acquisition process was stopped after 5000 monocytes, gated morphologically (bivariate plot of forward vs. side scatter) or on the basis of the CD14 bright fluorescence (FL3), were collected for each sample [20]. The neutrophil population was gated only morphologically (bivariate plot of forward vs. side scatter).…”

Role of granulocytes and monocytes in the polyvinyl chloride‐induced synthesis of interleukin 8, monocyte chemoattractant protein 1, and leukotriene B4

“…16,17 Conversely, CD15 is a major surface antigen on granulocytes but can also be found on monocytes. 18,19 The beads (Dynabeads M-450 CD14 and Dynabeads CD15) were used in the concentration recommended: 1 ϫ 10 7 beads/mL and 2 ϫ 10 7 beads/mL, respectively. The diluted blood containing the beads was incubated for 60 min at 4°C with gentle rotation.…”