“…For instance, Dang et al . used the strong fluorescence quenching ability of reduced graphene oxide (rGO) and DSN-assisted CSA to achieve the sensitive detection of VEGF mRNA in live cells [ 68 ] (Fig. 6 A).…”
Section: Application Of Csa For Fluorescent Biosensorsmentioning
confidence: 99%
“… Fig. 6 A Schematic diagram of sensitive detection of VEGF mRNA and live cell imaging based on DSN-assisted cycling [ 68 ]. B Schematic diagram of G-quadruple probe based on 2-aminopurine binding SDR for sensitive detection of miRNA-21 [ 72 ].…”
Section: Application Of Csa For Fluorescent Biosensorsmentioning
The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods.
Graphical Abstract
“…For instance, Dang et al . used the strong fluorescence quenching ability of reduced graphene oxide (rGO) and DSN-assisted CSA to achieve the sensitive detection of VEGF mRNA in live cells [ 68 ] (Fig. 6 A).…”
Section: Application Of Csa For Fluorescent Biosensorsmentioning
confidence: 99%
“… Fig. 6 A Schematic diagram of sensitive detection of VEGF mRNA and live cell imaging based on DSN-assisted cycling [ 68 ]. B Schematic diagram of G-quadruple probe based on 2-aminopurine binding SDR for sensitive detection of miRNA-21 [ 72 ].…”
Section: Application Of Csa For Fluorescent Biosensorsmentioning
The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods.
Graphical Abstract
“…Recently, various nanomaterials, including graphene oxide, 20 AuNPs, 21 mesoporous silicon nanoparticles, 22 ZnO, 23 and gold nanorods, 24 have attracted a great amount of attention for the assay of mRNA. Reduced graphene oxide nanosheet-assisted fluorescence probes were used to detect levels of p53 and p21 mRNA in HepG2 cells by Wang's group.…”
Section: Introductionmentioning
confidence: 99%
“…23 Liu et al reported a collaborative system of a fluorophore-labeled single-stranded probe, reduced graphene oxide and double-specific nuclease for the facile and efficient detection of VEGF mRNA and for imaging in living cells. 20 Although these two methods exhibited a relatively high sensitivity, the generated fluorescence signal may partly have originated from the background, thus resulting in an inaccuracy of the fluorescence signal. Therefore, there is a need to design a simple, rapid and low background assay for the in situ dynamic mapping of intracellular mRNA.…”
Monitoring the expression level of intracellular tumor suppressor gene of p21 mRNA is essential to reveal the progress and prognosis of tumor. Methods widely reported for the detection of p21...
“…To date, several approaches for the detection of VEGF have been reported, including chemiluminescence ( Long et al, 2018 ), fluorescence ( Dang et al, 2019 ), photoelectricity ( Da et al, 2018 ), colorimetry ( Zhang et al, 2017 ), electrochemistry ( Bozal-Palabiyik et al, 2019 ), and enzyme-linked immunosorbent assay (ELISA) ( Chattaraj et al, 2016 ). However, most methods require tedious steps or large-scale equipment with a point of care use.…”
In this study, a one-step electrochemical aptasensor was developed to detect the biomarker vascular endothelial growth factor (VEGF), an important protein in the pathogenesis of many retinal diseases, including age-related macular degeneration, diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. The aptamer has a good affinity and can rapidly identify and capture VEGF based on its unique structure. We designed a VEGF aptasensor based on the aptamer recognition and complex metallo nanoenzyme particles as an electron exchange center and bridge between capture DNA and electrode. The aptamers maintained the hairpin structure to avoid nonspecific surface adsorption and expose the capture sequence outwards when the target was inexistent. Conversely, the aptamers opened the hairpin structure to release space to accomplish binding between VEGF and DNA, resulting in increased impedance. The performance of the electrochemical aptasensor is detected by electrochemical impedance spectroscopy (EIS). The limit of detection by EIS was as low as 8.2 pg ml−1, and the linear range was 10 pg ml−1–1 μg ml−1. The electrochemical aptasensor also showed high specificity and reproducibility.
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