Monocarboxylate transporter 1 (MCT1) plays a direct role in short‐chain fatty acids absorption in caprine rumen

Kirat, Doaa; Masuoka, Junji; Hayashi, Hideaki; Iwano, Hidetomo; Yokota, Hiroshi; Taniyama, Hiroyuki; Kato, Shinichi

doi:10.1113/jphysiol.2006.115931

Cited by 92 publications

(90 citation statements)

References 38 publications

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“…This raises the possibility in our model that phloretin inhibited lactate transport out of H441 cells as well as inhibiting glucose uptake. Transport of acetate may also be mediated by MCT1 as proposed by an in vivo and in vitro study in the goat gastrointestinal tract [22]. In our study, acetate appearance in the apical reservoir was unaffected by the presence of phloretin, which could indicate the presence of phloretin-insensitive transporters.…”

Section: Transportsupporting

confidence: 51%

Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism

Kalsi

Baker

Fraser

et al. 2008

Pflugers Arch - Eur J Physiol

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Glucose in airway surface liquid (ASL) is maintained at low concentrations compared to blood glucose. Using radiolabelled [3 H]-D-glucose and [ 14 C]-Lglucose, detection of D-and L-glucose by high-performance liquid chromatography and metabolites by nuclear magnetic resonance, we found that glucose applied to the basolateral side of H441 human airway epithelial cell monolayers at a physiological concentration (5 mM) crossed to the apical side by paracellular diffusion. Transepithelial resistance of the monolayer was inversely correlated with paracellular diffusion. Appearance of glucose in the apical compartment was reduced by uptake of glucose into the cell by basolateral and apical phloretin-sensitive GLUT transporters. Glucose taken up into the cell was metabolised to lactate which was then released, at least in part, across the apical membrane. We suggest that glucose transport through GLUT transporters and its subsequent metabolism in lung epithelial cells help to maintain low glucose concentrations in human ASL which is important for protecting the lung against infection.

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Section: Transportsupporting

confidence: 51%

Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism

Kalsi

Baker

Fraser

et al. 2008

Pflugers Arch - Eur J Physiol

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“…This also indicates the decreasing gradient of transporters from the outside to the inside of the perineurium. Interestingly, a similar staining pattern with an intense expression of MCT1 only in the basal (contraluminal) part of stratified cell layers was reported in the stratified squamous epithelium in the ruminant forestomach, which transports a great amount of monocarboxylates (17). The apical (luminal) part of the stratified squamous epithelium, which lacks the MCT1 expression, does not develop a junctional apparatus between cells.…”

Section: Subcellular Localization Of Mct1 In the Peripheral Nervesmentioning

confidence: 86%

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

et al. 2008

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Peripheral nerves express GLUT1 in both endoneurial blood vessels and the perineurium and utilize glucose as a major energy substrate, as does the brain. However, under conditions of a reduced utilization of glucose, the brain is dependent upon monocarboxylates such as ketone bodies and lactate, being accompanied by an elevated expression of a monocarboxylate transporter (MCT1) in the blood-brain barrier. The present immunohistochemical study aimed to examine the expression of MCT1 in the peripheral nerves of mice. MCT1 immunoreactivity was found in the perineurial sheath and colocalized with GLUT1, while the endoneurial blood vessels expressed GLUT1 only. An intense expression of MCT1 in the perineurium was confirmed by Western blot and in situ hybridization analyses. Ultrastructurally, the MCT1 and GLUT1 immunoreactivities in the thick perineurium showed an intensity gradient decreasing towards the innermost layer. In neonates, the MCT1 immunoreactivity in the perineurium was intense, while the GLUT1 immunoreactivity was faint or absent. These findings suggest that peripheral nerves depend on monocarboxylates as a major energy source and that MCT1 in the perineurium is responsible for the supply of monocarboxylates to nerve fibers and Schwann cells.

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“…Expression of MCT1 mRNA was detected in all tissues sampled from the GIT of goats (Kirat et al, 2006b), pre-ruminating calves (Kirat et al, 2005) and sheep (Kirat et al, 2006a) by RT-PCR, with highest expression of the protein in the rumen and reticulum, moderate expression in the omasum, cecum, colon and abomasum, and very low expression in the small intestine, suggesting a positive association between SCFA concentration and MCT1 expression. In addition, localization of the transport protein to the stratum basale and stratum spinosum of the caprine rumen epithelium, the two cell layers closest to the blood-rumen interface, further indicated a role of MCT1 in the transport of SCFA across the rumen epithelium into the blood (Kirat et al, 2006b).…”

Section: Introductionmentioning

confidence: 95%

“…Similarly, through use of inhibitory molecules and a series of immunohistochemical and RT-PCR studies, Kirat et al (2006b) demonstrated that absorption of SCFA, particularly acetate and propionate by the GIT epithelium is regulated by MCT1. Expression of MCT1 mRNA was detected in all tissues sampled from the GIT of goats (Kirat et al, 2006b), pre-ruminating calves (Kirat et al, 2005) and sheep (Kirat et al, 2006a) by RT-PCR, with highest expression of the protein in the rumen and reticulum, moderate expression in the omasum, cecum, colon and abomasum, and very low expression in the small intestine, suggesting a positive association between SCFA concentration and MCT1 expression.…”

Section: Introductionmentioning

confidence: 99%

Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

Connor

Baldwin

et al. 2010

Animal

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The gastrointestinal tract (GIT) has multiple functions including digestion, nutrient absorption, secretion of hormones and excretion of wastes. In the ruminant animal, development of this organ system is more complex than that of the monogastric animal due to the necessity to establish a fully functional and differentiated rumen, in which a diverse microbial population of bacteria, fungi and protozoa support fermentation and digestion of dietary fiber. Central to the goal of animal scientists to enhance nutrient uptake and production efficiency of ruminants is the need for a comprehensive understanding of GIT development, as well as conditions that alter the digestion process. The relatively recent availability of genome sequence information has permitted physiological investigations related to the process of digestion for many agriculturally important species at the gene transcript level. For instance, numerous studies have evaluated the expression of ruminant GIT genes to gain insight into mechanisms involved in normal function, physiology and development, such as nutrient uptake and transport across the epithelial cell barrier throughout the alimentary canal, maintenance of rumen pH, and regulation of GIT motility and cell proliferation. Further, multiple studies have examined the effects of dietary modification, including feeding of supplemental fat, starch and protein, or a forage-v. concentrate-based diet on expression of critical gene pathways in the gut. In addition, the expression of genes in the GIT in response to disease, such as infection with gastrointestinal parasites, has been investigated. This review will summarize some of the recent scientific literature related to the gene expression in the GIT of ruminants, primarily cattle, sheep and goats, as it pertains to normal physiology, and dietary, developmental, and disease effects to provide an overview of critical proteins participating in the overall digestive processes, and their physiological functions. Recent findings from our laboratory will be highlighted also related to expression of the glucagon-like peptide two-hormone pathway in the GIT of dairy cattle during in various stages of the development and lactation, alterations in gene pathways associated with the rumen development and differentiation in the weaning calf, and genes of the GIT responding to Ostertagia, a common nematode infection of the cattle. Finally, prospective areas of investigation will be highlighted.

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Monocarboxylate transporter 1 (MCT1) plays a direct role in short‐chain fatty acids absorption in caprine rumen

Cited by 92 publications

References 38 publications

Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism

Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

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