Monoclonal Antibodies for Specific Immunoperoxidase Detection of Campylobacter pylori

Negrini, Riccardo; Lisato, Laura Camilla; Cavazzini, Luigi; Maini, P.; Gullini, S.; Basso, Olga; Lanza, Giovanni; Garófalo, Maria Antonieta Rissato; Nenci, Italo

doi:10.1016/0016-5085(89)91565-5

Cited by 43 publications

(28 citation statements)

References 29 publications

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“…The whole-cell ELISA method was used [26] for the assay of reactivity to surface antigen of H. pylori. Microtitration plates were coated with 100 pl of a suspension of bacterial cells (8 X lo7 cfu/ml) in Na2C03 0.38% solution containing methylglioxal (Sigma) 0.3%v/v and incubated overnight at 4°C.…”

Section: Elisamentioning

confidence: 99%

Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells

Osaki

Yamaguchi

Taguchi

et al. 1998

Journal of Medical Microbiology

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Section: Elisamentioning

confidence: 99%

Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells

Osaki

Yamaguchi

Taguchi

et al. 1998

Journal of Medical Microbiology

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“…pylori (14). The present study was undertaken to evaluate the possible capacity of monoclonal antibodies to be used in competitive ELISA systems for the detection of human antibodies against single H. pylori antigens.…”

mentioning

confidence: 99%

Serodiagnosis of Helicobacter pylori-Associated Gastritis with a Monoclonal Antibody Competitive Enzyme-Linked Immunosorbent Assay

Negrini

Zanella

Savio

et al. 1992

Scandinavian Journal of Gastroenterology

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Forty-nine monoclonal antibodies against Helicobacter pylori were screened to investigate their capacity to be used in enzyme-linked immunosorbent assay (ELISA) competitive systems for the serodiagnosis of Helicobacter pylori infection. On the basis of the inhibition pattern showed by the sera of five infected patients, the antibodies were subdivided into five groups. The immunoblotting analysis showed that the antibodies recognized a total of nine different antigenic determinants. In a study of the reaction of the antibodies with 12 isolates of H. pylori a total of 9 antigenic profiles were identified. Two monoclonal antibodies, HpN44 and HpN45, which recognized a 64-kD protein, were inhibited by all 5 positive sera. Antibody HpN45 was labeled with horseradish peroxidase, and the competitive ELISA was compared with an ordinary indirect ELISA in a study of 102 patients undergoing gastroscopy. Seventy-three patients proved to be infected by H. pylori according to urease or histologic tests. The sensitivity and specificity were 90.4% and 89.6%, respectively, for the indirect ELISA and 100% and 89.6% for the HpN45 competitive assay. The three patients who were 'false seropositive' with both serologic tests had atrophic gastritis. The high diagnostic performance and simplicity of the HpN45 monoclonal competitive ELISA make it suitable for routine serodiagnosis of H. pylori infection.

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“…C3-9 (IgG1 subclass) against activated complement factor C3 and B13.9 against CD66b (granule marker) have been used previously for immunohistochemical studies [22]. 6H3, a mAb against diand/or trimeric Le x structures [23], was a kind gift of Dr. Ben Appelmelk (VU University Medical Centre, Amsterdam, the Netherlands). Immortalized human hepatocytes (IHH) were a kind gift of Dr. Han Moshage (University of Groningen, the Netherlands) [24].…”

Section: Methodsmentioning

confidence: 99%

“…Cryosections were double-labeled with anti-AGP and antilactoferrin or anti- 1 and 4) or from patients suffering from acute trauma (lanes 2 and 5) and of PMN AGP (lanes 3 and 6) were immunostained with rabbit anti-human AGP IgG or the diand/or trimeric Lewis X -specific antibody 6H3. Lane 7, Trimeric Lewis X -albumin conjugate, a positive control for 6H3 [23]. (c) AGP secretion by activated PMN.…”

Section: Subcellular Localization Of Agp In Pmnmentioning

confidence: 99%

Activated human PMN synthesize and release a strongly fucosylated glycoform of α1-acid glycoprotein, which is transiently deposited in human myocardial infarction

Poland¹,

Vallejo²,

Niessen

et al. 2005

Journal of Leukocyte Biology

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␣ 1 -Acid glycoprotein (AGP) is a major acute-phase protein present in human plasma as well as in polymorphonuclear leukocytes (PMN). In this report, we show that PMN synthesize a specific glycoform of AGP, which is stored in the specific and azurophilic granules. Activation of PMN results in the rapid release of soluble AGP. PMN AGP exhibits a substantially higher apparent molecular weight than plasma AGP (50 -60 kD vs. 40 -43 kD), owing to the presence of strongly fucosylated and sialylated polylactosamine units on its five Nlinked glycans. PMN AGP is also released in vivo from activated PMN, as appeared from studies using well-characterized myocard slices of patients that had died within 2 weeks after an acute myocardial infarction. AGP was found deposited transiently on damaged cardiomyocytes in areas with infiltrating PMN only. It is interesting that this was inversely related to the deposition of activated complement C3. Strongly fucosylated and sialylated AGP glycoforms have the ability to bind to E-selectin and to inhibit complement activation. We suggest that AGP glycoforms in PMN provide an endogenous feedback-inhibitory response to excessive inflammation. J. Leukoc. Biol. 78: 453-461; 2005.

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Monoclonal Antibodies for Specific Immunoperoxidase Detection of Campylobacter pylori

Cited by 43 publications

References 29 publications

Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells

Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells

Serodiagnosis of Helicobacter pylori-Associated Gastritis with a Monoclonal Antibody Competitive Enzyme-Linked Immunosorbent Assay

Activated human PMN synthesize and release a strongly fucosylated glycoform of α1-acid glycoprotein, which is transiently deposited in human myocardial infarction

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