Monoclonal Antibody-Based Assay for Alt a1, a Major Alternaria Allergen

Potnoy, Jay; Pacheco, Freddy; Landuyt, Julie; Barnes, Charles S.

doi:10.1016/s1081-1206(10)63110-9

Cited by 30 publications

(21 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding was supported recently in the United Kingdom, where elevated concentrations of Alternaria, due to thunderstorms, were associated with an increase in asthma attacks [45•]. Because Cladosporium, Epicoccum, and Stemphylium have epitopes that cross react with Alternaria [46][47][48], each likely has a similar, but perhaps less well documented, association with asthmatic episodes.…”

Section: Fungi As Triggers Of Asthmamentioning

confidence: 74%

Importance of mold allergy in asthma

Portnoy

Barnes²,

Kennedy³

2008

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

Fungal exposure is hypothesized (controversially) to contribute to asthma development and to trigger symptoms in patients with asthma. The ubiquity of environmental fungal exposure makes a careful review of evidence essential. Evidence that exposure to high concentrations of fungal spores, antigens, or metabolites is associated with asthma development is limited. However, because mechanisms of asthma genesis are poorly understood, so too are the mechanisms of this potential association. This association is not proof of causality. Stronger evidence supports the hypothesis that fungal exposure triggers symptoms in asthmatic individuals. Proposed mechanisms have been tested and correlations between exposure and symptoms demonstrated. Though some correlations remain speculative, controlled studies could test such hypotheses. Because asthma is common and fungal exposure is ubiquitous, it is surprising that asthmatics don't have more symptoms when exposed to fungi. Fortunately, symptoms are dose dependent, creating an opportunity to develop clinically effective interventions. Given the right guidance, even patients with severe asthma can create healthy indoor environments.

show abstract

Section: Fungi As Triggers Of Asthmamentioning

confidence: 74%

Importance of mold allergy in asthma

Portnoy

Barnes²,

Kennedy³

2008

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

show abstract

“…24 A variety of techniques have been developed in an attempt to quantitate indoor fungal exposure, although these have not been fully characterized or developed as have sampling measures for other indoor allergens (eg, house dust mites, cat dander). Allergen-specific immunoassays that use mAbs to specific allergens have been used to quantitate indoor allergen exposure to Alternaria allergens 25,26 ; however, these assays have not yet achieved the same reliability as have similar assays for other allergens.…”

Section: Aerobiology Of Alternariamentioning

confidence: 99%

Alternaria-induced asthma☆

Bush

Prochnau

2004

Journal of Allergy and Clinical Immunology

299

215

View full text Add to dashboard Cite

“…The major allergen of A. alternata , Alt a1, is a 31-kDa glycoprotein [17]. Among the C. herbarum allergens, a 54-kDa glycoprotein, designated Cla h II, has been characterized in detail for its protein and carbohydrate parts [18].…”

Section: Discussionmentioning

confidence: 99%

<i>Cladosporium herbarum</i> and <i>Pityrosporum ovale </i>Allergen Extracts Share Cross-Reacting Glycoproteins

Leino

Reijula²,

Mäkinen-Kiljunen³

et al. 2006

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Patients sensitized to airborne fungi such as Alternaria alternata and Cladosporium herbarum often also show positive skin prick test results and specific serum IgE antibodies to a yeast, Pityrosporum ovale. We examined whether part of the IgE binding to these fungi is explained by cross-reacting mould and yeast allergens. Methods: Serum samples from 36 patients with positive skin prick test to A. alternata or C. herbarum were analyzed for IgE antibodies to fungal extracts by ELISA and immunoblot analysis. Cross-reactivity between mould and yeast extracts was studied by ELISA and immunoblot inhibition assays. In further analysis, the mannan-containing glycoproteins were removed from the yeast extract by concanavalin A-Sepharose chromatography, and the IgE binding properties of the extracts were compared. Results: SerumIgE reactivity to P. ovale was found in 40% of the mould-sensitized patients. The IgE antibody binding to A. alternata and C. herbarum moulds was partially inhibited by the yeast P. ovale in ELISA and immunoblot inhibition assays. When the glycoproteins were removed from the extract, cross-reactivity was markedly reduced. Conclusion: Part of the IgE binding to mould and yeast allergen extracts is due to cross-reacting glycoproteins. False-positive IgE and skin prick test results should be taken into account in the diagnosis of mould allergy.

show abstract

Monoclonal Antibody-Based Assay for Alt a1, a Major Alternaria Allergen

Cited by 30 publications

References 19 publications

Importance of mold allergy in asthma

Importance of mold allergy in asthma

Alternaria-induced asthma☆

<i>Cladosporium herbarum</i> and <i>Pityrosporum ovale </i>Allergen Extracts Share Cross-Reacting Glycoproteins

Contact Info

Product

Resources

About