Monoclonal Antibody TSd-1 is Specific to Elongating and Matured Spermatids in Testis of Common Tree Shrew(Tupaia glis).

Abstract

ABSTRACT. The monoclonal antibody (MAb), named TSd-1, specific to spermatogenic cells of the common tree shrew (Tupaia glis) was established and characterized using immunohistochemistry and immunoblotting. MAb TSd-1 reacted with elongating and matured spermatids in a stage-dependent manner. TSd-1 recognized a 94 kilodalton (kDa) peptide in the plasma membrane and cytosol. Additionally, an extremely weak 107 kDa band was detected only in the cytosol. The reactions were not detected in round spermatids. In elongating Stage VI spermatids, the plasma membrane and the granular structure within the cytoplasm were intensely positive, and most intense after the appearance of new round spermatids in the lower layer (Stage I). The reactions were observed neither in the other organs of the common tree shrew nor in the testes of other animals, indicating that TSd-1 antigen is specific to the spermatogenic cells of the common tree shrew, and may act on elongating or matured spermatids. -KEY WORDS : monoclonal antibody, spermatogenesis, spermatozoa, tree shrew.

Morphological characteristics and cryodamage of Chinese tree shrew (<I>Tupaia belangeri chinensis</I>) sperm

“…树鼩是一种昼行、树栖的小型哺乳动物, 曾被 定义为攀鼩目、 灵长目 (Liu et al, 2001;Wong & Kaas, 2009), 目前对树鼩的分类地位仍然存在争议。但无 论如何, 树鼩是最接近于灵长类的小型哺乳动物, 具有许多灵长类动物共有的特征 (Poonkhum et al, 2000)。 树鼩在人类医学及生物学研究方面具有重要 地位, 如神经生物学 (Remple et al, 2007)、人类肝炎 病毒感染 (Xu et al, 2007;Li et al, 2008)、视觉系统发 育 (Poveda & Kretz, 2009)及群体阶层应激 (Kozicz et al, 2008;Zambello et al, 2010)等。目前, 有关雄性树 鼩生殖生物学研究的报道较少 (Collins et al, 2007)。 Collins et al 于 1982 年报道了雄性树鼩生殖道的解 剖学特征和生理功能 (Collins et al, 1982); 随后, 有 研究报道了雄性树鼩从出生到性成熟过程的生殖 器官生长与发育 (Collins & Tsang, 1987); 精子细胞 在睾丸内的成熟过程 (Maeda et al, 1998); 雄性树鼩 精子的形成和染色质凝聚 (Suphamungmee et al, 2008) 等结果。然而, 树鼩睾丸、精子形态结构特性的研 究仍然很有限。我们曾报道树鼩精子可以被冷冻保 存 (Ping et al, 2011), 但是精子冷冻后的超微结构变 化尚不清楚。为此, 本研究分析树鼩的睾丸、附睾 精子的形态学结构特征, 以及树鼩精子冷冻复苏后 的结构变化。 1 材料和方法…”