Monocyte-derived dendritic cell subpopulations use different types of matrix metalloproteinases inhibited by GM6001

Cell-type deconvolution with immune pathways identifies gene networks of host defense and immunopathology in leprosy

“…MMP12 and its coexpressed genes were linked to "response to wounding," "chemotaxis," "cell proliferation," and "tissue morphogenesis" pathways. In DC, MMP-12 is induced by a combination of inflammatory cytokines, including TNF-α, IL-1β, IL-6 and GM-CSF (31). MMP-12 is also known to be expressed in monocytes, macrophages, and fibroblasts.…”

“…Although the major substrate for MMP-12 is elastin, MMP-12 is able to degrade a broad spectrum of substrates, such as type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, α-1-antitrypsin, plasminogen, and myelin basic protein, which is related to nerve damage in the disease (32). MMP-12 contributes to cell migration (33) of DC and leukocytes (31,34). The ability of MMP-12 to cleave off IFN-α receptor 2-binding sites (35) blocks the action of type I IFN, which in previous work we showed blocks antimicrobial responses in leprosy (16).…”

Transforming Growth Factor-Beta and Matrix Metalloproteinases: Functional Interactions in Tumor Stroma-Infiltrating Myeloid Cells

“…Dendritic cells produce MMP-1, MMP-2, MMP-3, MMP-9, MMP-12, MMP-14 and MMP-19 [10, 119, 120]. Overproduction of these MMPs by DCs during infection is associated with a decrease in endothelial PECAM-1 and VE-cadherin in vascular endothelium in vitro and in vivo , which may contribute to the marked increase in vascular permeability.…”

Mesenchymal Stromal Cell-Like Cells Set the Balance of Stimulatory and Inhibitory Signals in Monocyte-Derived Dendritic Cells

“…In a previous work we have characterized the expression patterns of MMP enzymes and TIMP inhibitors in monocytes and monocyte-derived DC subpopulations [31] and discovered that the chemokine driven migration of activated DC is regulated by the expression of the voltage gated sodium channel Nav1.7 [32]. Based on these findings in this study we aimed to anti-mycoticum/anti-bioticum solution (Hyclone, South Logan, Utah) in low glucose DMEM.…”