Monolithic columns in high-performance liquid chromatography

Polyhedral oligomeric silsesquioxanes as functional monomer to prepare hybrid monolithic columns for capillary electrochromatography and capillary liquid chromatography

“…Each column was flushed for at least 30 min before measuring back pressure, and measurements were taken twice at each flow rates between 50 and 600 nL min −1 . The superficial permeability values [19] for these monoliths were calculated using Darcy's equation, K = (FÁL)/( r 2 P), where K is the permeability, F the flow rate of mobile phase, Á the viscosity of the mobile phase, L the column length, r the inner radius of the capillary and P the pressure drop across the column. Electrochromatographic experiments were performed on an Agilent CE system (Hewlett-Packard, Waldbronn, Germany) equipped with a UV detector.…”

“…These excellent advantages have made the monolithic columns an attractive alternative to the packed and open-tubular columns in the field of microscale separation, including CEC and capillary liquid chromatography (cLC) [16][17][18][19][20]. The process of monolithic materials affords a variety of surface chemistries for the requirement of small and large molecules in several separation modes.…”

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

“…Silica C 18 (Chocholouš et al , 2013, polymer-based (polyacrylamide gels), synthetic organic materials (acrylate resins), natural polymers (cellulose), and preparative columns have been used for SIC (Šatínský et al 2003;Guiochon 2007;Jangbai et al 2012). The SIC separations are performed using sample volumes and flow rates between 10 and 200 µL and from 0.5 to 2.4 mL min −1 , respectively, and the available pressures of the relief valve are currently 250-500 psi (Idris 2014).…”

Development and characterisation of switchable polyaniline-functionalised flow-through capillary monoliths

“…They have been used extensively in chromatography over the last 20 years 16,17 because of the high inter-pore connectivity which allows efficient separations to be achieved at high ow rates due to the low pressure-drop across the column. Continuous porous PANI monoliths have been prepared in microuidic housings as recently described by Gorey et al 18 who demonstrated the fabrication of an inverse-opal conducting PANI monolith via electrochemical growth of PANI within the interstices of a sacricial PS colloidal crystal template.…”