Monolithic porous layer open tubular (monoPLOT) columns for low pressure liquid chromatography of proteins

Nesterenko, Ekaterina P.; Yavorska, Oksana; Macka, Mirek; Yavorskyy, Alexander; Paull, Brett

doi:10.1039/c0ay00649a

Cited by 25 publications

(27 citation statements)

References 28 publications

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“…Where polymerization conditions are limited by either time or dynamic flow, a porous layer structure results, rather than complete polymerization throughout the capillary. Early attempts to produce photoinitiated monoPLOT columns 26 were conducted using simple silanization of only the capillary walls with trimethoxysilylpropyl methacrylate. Although showing promising results, this approach would often result in a nonuniform layer thickness or areas of the capillary wall, where little or no polymerization would occur.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…It was shown that columns with a layer thickness ranging from 2 μm to 25 μm were obtained; however, again, only short columns (<11 cm) could be produced using this approach, as layer thickness would vary along the column length, decreasing with the increase of distance from the light source increased, because of attenuation within the silica medium. Following this work,Nesterenko et al 26 reported the preparation of slightly longer monolithic porous layer open tubular (monoPLOT) columns using an automated UV scanning technique. In this work, capillaries filled with polymerization mixture were repeatedly exposed to light from the scanning source at a wavelength of 365 nm.…”

Section: Introductionmentioning

confidence: 99%

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Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

et al. 2012

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ABSTRACT:An automated column fabrication technique that is based on a ultraviolet (UV) light-emitting diode (LED) array oven, and provides precisely controlled "in-capillary" ultraviolet (UV) initiated polymerization at 365 nm, is presented for the production of open tubular monolithic porous polymer layer capillary (monoPLOT) columns of varying length, inner diameter (ID), and porous layer thickness. The developed approach allows the preparation of columns of varying length, because of an automated capillary delivery approach, with precisely controlled and uniform layer thickness and monolith morphology, from controlled UV power and exposure time. The relationships between direct exposure times, intensity, and layer thickness were determined, as were the effects of capillary delivery rate (indirect exposure rate), and multiple exposures on the layer thickness and axial distribution. Layer thickness measurements were taken by scanning electron microscopy (SEM), with the longitudinal homogeneity of the stationary phase confirmed using scanning capacitively coupled contactless conductivity detection (sC4D). The new automated UV polymerization technique presented in this work allows the fabrication of monoPLOT columns with a very high column-to-column production reproducibility, displaying a longitudinal phase thickness variation within ±0.8% RSD (relative standard deviation).

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

et al. 2012

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“…1,2 The application of C4D in what has been termed capillary 'scanning mode' can be used to differentiate between monoliths of different morphologies, with Connolly et al [15][16][17] having shown that C4D can be used successfully as a non-invasive method for the determination of monolith longitudinal homogeneity, determination of coating stability and location, as well as identi cation of monolith or packing material voids.…”

Section: C4d Responsementioning

confidence: 99%

“…The polymerisation mixture was pumped through the capillary at a linear ow rate of 0.5 mm s 1 . After flow was established and the C4D response had stabilised, the air oven temperature was increased to 60 0 C. The C4D response rose with an increase in temperature and once the oven temperature stabilised the C4D response also became steady state.…”

Section: C4d Measurements During Thermally Initiated Polymerisationmentioning

confidence: 99%

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In-process phase growth measurement technique in the fabrication of monolithic porous layer open tubular (monoPLOT) columns using capacitively coupled contactless conductivity

Collins

Nesterenko

Brabazon

et al. 2013

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A technique for the in-process measurement of polymer stationary phase growth inside fused silica capillaries during the fabrication of monolithic porous layer open tubular (monoPLOT) columns is presented. In this work, capacitively coupled contactless conductivity detection (C4D) was applied as an online measurement tool for porous polymer layer growth within fused silica capillaries. The relationship between effective capillary diameter and C4D response was investigated for two polymers, butyl methacrylate-ethylene dimethacrylate (BuMA-EDMA) and polystyrene-divinylbenzene (PS-DVB) over a range of capillary diameters and layer thicknesses. The described technique can be used with both thermal and photo-initiated approaches for monoPLOT fabrication and provides an accurate, realtime measurement of the porous layer growth within the capillary, which should vastly improve columnto-column reproducibility. The technique was shown to be very precise, with a measured %RSD < 10%.

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Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

Kubáň

Hauser

2012

Electrophoresis

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The developments in the field of capacitively coupled contactless conductivity detection in the approximate period from July 2010 to June 2012 are traced. Few reports concerning fundamental studies or new detector designs have appeared. On the other hand, applications in standard CZE are flourishing and contactless conductivity measurements are increasingly being employed as part of novel or more sophisticated experimental systems. Work on the lab-on-chip devices integrating contactless conductivity detection is continuing. A range of reports on the use of the simple yet powerful detection technique of contactless conductivity measurements in chromatographic separation as well as for analytical methods not including a separation step have also appeared.

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Monolithic porous layer open tubular (monoPLOT) columns for low pressure liquid chromatography of proteins

Cited by 25 publications

References 28 publications

Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

In-process phase growth measurement technique in the fabrication of monolithic porous layer open tubular (monoPLOT) columns using capacitively coupled contactless conductivity

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

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