Monomer Elution from Three Resin Composites at Two Different Time Interval Using High Performance Liquid Chromatography—An In-Vitro Study

Abstract

Esthetics, improved colour stability and ease of contour have made photo-activated resin based restorative materials being widely used in routine dental clinical practice. Perhaps improper and inadequate polymerization of resin based composite material might lead to elution of monomer. Thus, the aim of the current study was to quantify the monomer elution from three resin composites. The intended analysis was made using high performance liquid chromatography (HPLC) at two different time periods. Three different materials that were investigated in the current study included Swiss Tech resin composite (Group A), Ceram X (Group B) and Beautifil Injectable composite (Group C). Ten cylindrical samples were fabricated in each study group. In 75% wt of ethanol, the samples were ingressed immediately and stored at room temperature. A 0.5 mL of the samples was assessed at pre-defined time intervals at 24 h and 7th day. Later, assessment of the samples was performed with HPLC and the data was analyzed using statistical test. Bisphenol A-glycidyl methacrylate (Bis-GMA), Triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA) and Urethane dimethacrylate (UDMA) were quantified in the samples. When analyzing the release monomer, it was found that at the end of 24 h Bis-GMA was eluted more in the injectable resin composite whereas, TEGDMA was eluted from Swiss Tech and Ceram X resin composites. At the end of the 7th day it was evident that Bis-GMA was eluted maximum in all the three resin composites. Thus, monomer release was found to be evident among all three resin composites and it is of utmost important to be assessed in routine clinical practice.

Detection of Leachable Components from Conventional and Dental Bulk-Fill Resin Composites (High and Low Viscosity) Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method

“…With higher monomer concentrations in the samples stored for 1 month compared to those stored for 24 h and 7 days, it was found in the present study that increasing the storage time resulted in higher amounts of Bis GMA and DMA BEE elution for all Tetric and Filtek composites, as noted in the study by Janani et al [ 46 ]. Nazar at al.…”

“…also used high-performance liquid chromatography analysis and reported that longer storage times resulted in statistically significant increases in BisGMA and UDMA amounts for both Tetric and Filtek materials [ 47 ]. There are few studies investigating the long-term elution of monomers over 1, 3, and 12 months using liquid chromatography tandem mass spectrometry and high-performance liquid chromatography [ 16 , 46 ]. However, the long-term effects of residual monomers on biocompatibility are still unclear.…”

Evaluation of the Impact of Irradiance Lamps and Storage Media on Elution of TEGDMA from Dental Composites

“…27 The U.S. Food and Drug Administration (FDA) has graded a 75% ethanolwater solution as clinical oral stimulating liquid and it has been used in several studies. 28 In our study, we incorporated a 70% ethanol-water solution as one of our storage solutions and the elution of TEGDMA was high in ethanol as compared to other solutions. It can further be justified by the fact that ethanol has the ability to penetrate within the unreacted monomer and widens the gap within the polymer chains, allowing the soluble compounds to elute.…”

Influence of Commonly Used Endodontic Irrigants on the Setting Time and Metal Composition of Various Base Endodontic Sealers

“…Metal compounds, though, are present in all endodontic sealers, and their values can vary on interaction with different irrigants used in endodontics, eventually influencing the outcome in the long run. One of the limitations of the present study is the use of a tooth model; though it better simulates clinical conditions, the results achieved could vary when done in vivo, based on a multitude of factors such as clinician’s irrigation preferences, treatment protocol, endodontic irrigant concentration, the tooth of treatment, and canal morphology [ 52 , 53 ]. Though the use of different final irrigation protocols in the assessed teeth would not necessarily replicate the clinical scenario, it was nevertheless conducted since the primary aim was to assess if endodontic irrigants present on the internal dentinal surface had an influence on the properties of the endodontic sealers.…”