Mononuclear cells morphology for cells discrimination by the angular structure of scattered light

Loiko, Valery A.; Ruban, Gennady I.; Gritsai, Olga A.; Berdnik, Vladimir V.; Гончарова, Н. В.

doi:10.1615/ichmt.2007.confelectromagligscat.290

Cited by 9 publications

(10 citation statements)

References 6 publications

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“…Lymphocytes have a large nucleus that usually occupies 80% of the whole cell [22]. They have a spherical shape and range in size from 6 µm to 7 µm.…”

Section: Resultsmentioning

confidence: 99%

Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

et al. 2013

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The use of optical dielectrophoresis (ODEP) to manipulate microparticles and biological cells has become increasingly popular due to its tremendous flexibility in providing reconfigurable electrode patterns and flow channels. ODEP enables the parallel and free manipulation of small particles on a photoconductive surface on which light is projected, thus eliminating the need for complex electrode design and fabrication processes. In this paper, we demonstrate that mouse cells comprising melan-a cells, RAW 267.4 macrophage cells, peripheral white blood cells and lymphocytes, can be manipulated in an opto-electrokinetics (OEK) device with appropriate DEP parameters. Our OEK device generates a non-rotating electric field and exerts a localized DEP force on optical electrodes. Hitherto, we are the first group to report that among all the cells investigated, melan-a cells, lymphocytes and white blood cells were found to undergo self-rotation in the device in the presence of a DEP force. The rotational speed of the cells depended on the voltage and frequency applied and the cells' distance from the optical center. We discuss a possible mechanism for explaining this new observation of induced self-rotation based on the physical properties of cells. We believe that this rotation phenomenon can be used to identify cell type and to elucidate the dielectric and physical properties of cells.

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“…Lymphocytes have a large nucleus that usually occupies 80% of the whole cell [22]. They have a spherical shape and range in size from 6 µm to 7 µm.…”

Section: Resultsmentioning

confidence: 99%

Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

et al. 2013

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“…For lymphocytes we used a cell nucleus area of 30 lm 2 that has been reported in previously published literature (24). Using the relationship between LET (in units of keV/lm), dose ¼ D (in units of Gy) and fluence ¼ F (in units of lm 2 ) as D ¼ 6.24 F 3 L, the mean number of particle hits per cell nucleus (25) is given by …”

Section: Discussionmentioning

confidence: 99%

Induction of Chromosomal Aberrations at Fluences of Less Than One HZE Particle per Cell Nucleus

et al. 2014

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The assumption of a linear dose response used to describe the biological effects of high LET radiation is fundamental in radiation protection methodologies. We investigated the dose response for chromosomal aberrations for exposures corresponding to less than one particle traversal per cell nucleus by high energy and charge (HZE) nuclei. Human fibroblast and lymphocyte cells where irradiated with several low doses of <0.1 Gy, and several higher doses of up to 1 Gy with O (77 keV/m), Si (99 keV/m), Fe (175 keV/m), Fe (195 keV/m) or Fe (240 keV/m) particles. Chromosomal aberrations at first mitosis were scored using fluorescence in situ hybridization (FISH) with chromosome specific paints for chromosomes 1, 2 and 4 and DAPI staining of background chromosomes. Non-linear regression models were used to evaluate possible linear and non-linear dose response models based on these data. Dose responses for simple exchanges for human fibroblast irradiated under confluent culture conditions were best fit by non-linear models motivated by a non-targeted effect (NTE). Best fits for the dose response data for human lymphocytes irradiated in blood tubes were a NTE model for O and a linear response model fit best for Si and Fe particles. Additional evidence for NTE were found in low dose experiments measuring H2AX foci, a marker of double strand breaks (DSB), and split-dose experiments with human fibroblasts. Our results suggest that simple exchanges in normal human fibroblasts have an important NTE contribution at low particle fluence. The current and prior experimental studies provide important evidence against the linear dose response assumption used in radiation protection for HZE particles and other high LET radiation at the relevant range of low doses.

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“…In morphological studies, the measurement method such as flow cytometry [ 38 ], blood smear, or detailed examination with microscopic study are broadly employed to analyze the cell size of CTCs in comparison to normal cells. The size measurement of cell is normally interpreted in cell area, length, width, and shape features.…”

Section: Comparison Of Biophysical and Biomechanical Properties Bementioning

confidence: 99%

“…Despite the differences in these measurement methods, they provide similar quantitative value for both CTCs and normal blood cell measurement, respectively. For example, normal human erythrocyte, also called red blood cells (RBC), is found to have biconcave disks with the diameter of approximately 6 to 8 μ m [ 38 – 40 ]. White blood cells (WBC) are derived into two groups, which are granulocytes and agranulocytes.…”

Section: Comparison Of Biophysical and Biomechanical Properties Bementioning

confidence: 99%

“…Meanwhile, agranulocytes such as lymphocytes vary widely in size where the small lymphocytes are 7–10 μ m while large lymphocytes are approximately 14–20 μ m in diameter. Monocytes are among the largest leukocytes with a diameter of 15 to 25 μ m in blood smears [ 38 , 41 – 43 ]. In contrast to the mentioned blood cells, the size of CTCs reported in the literature is generally larger than normal blood cells, ranging from 17 μ m to 52 μ m. The clinical relevance of this taxonomy has been investigated and confirmed by many research groups [ 44 – 47 ].…”

Section: Comparison Of Biophysical and Biomechanical Properties Bementioning

confidence: 99%

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Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Low

Abas

2015

BioMed Research International

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Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite challenging due to their large morphological variability and the rare presence of CTCs within the blood. Numerous methods have been employed and discussed in the literature for CTCs separation. In this paper, we will focus on label free CTCs isolation methods, in which the biophysical and biomechanical properties of cells (e.g., size, deformability, and electricity) are exploited for CTCs detection. To assess the present state of various isolation methods, key performance metrics such as capture efficiency, cell viability, and throughput will be reported. Finally, we discuss the challenges and future perspectives of CTC isolation technologies.

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Mononuclear cells morphology for cells discrimination by the angular structure of scattered light

Cited by 9 publications

References 6 publications

Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

Induction of Chromosomal Aberrations at Fluences of Less Than One HZE Particle per Cell Nucleus

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

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