Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Dasig, D.; Lyon, J.; Behr, Barry; Milki, Amin A.

doi:10.1007/s10815-004-8758-2

Cited by 25 publications

(23 citation statements)

References 15 publications

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“…Similar reports on neonatal data derived from 1PN embryos in cIVF and ICSI have been reported in the literature [4,5,7,8]. Gras and Trounson [7] and Reichman et al [5] have reported similar findings on normal birth weight, the weeks of gestation, and a birth of a healthy child.…”

Section: Discussionsupporting

confidence: 77%

“…Gras and Trounson [7] and Reichman et al [5] have reported similar findings on normal birth weight, the weeks of gestation, and a birth of a healthy child. Staessen et al [4] and Dasig et al [8] also described the delivery of healthy babies without neonatal data. Previous studies regarding live birth babies derived from 1PN embryos have reported no major malformations.…”

Section: Discussionmentioning

confidence: 99%

“…Although such abnormal pronuclear-stage embryos are considered unsuitable for transfer to patients, they are occasionally transferred and a few attempts for normal pregnancies have been reported including embryos derived from 1PN zygotes [4,5,7,8]. However, formation rate and clinical pregnancy rate of blastocysts derived from 1PN zygotes were unclear.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Itoi¹,

Asano²,

Shimizu³

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study is to examine the clinical outcomes of blastocysts derived from human singlepronucleate (1PN) embryos after conventional in vitro fertilization (cIVF) and intracytoplasmic sperm injection (ICSI) cycles. Methods This was a retrospective study at a reproductive center of a hospital. To evaluate embryo quality and clinical outcomes, cIVF or ICSI cycles with one or more 1PN embryos were compared with same cycles with 2PN embryos (control cycles).Results A total of 623 cycles (426 cIVF cycles and 197 ICSI cycles) were treated with cIVF or ICSI. The single pronuclear status rate was similar between cIVF (22.1 %) and ICSI (25.1 %) cycles. Although the development rates of 1PN embryos on day 3 and day 5/6 in cIVF were significantly higher than those in ICSI, those of 1PN embryos in cIVF were significantly lower compared to 2PN embryos (p<0.01). Nonetheless, the ongoing pregnancy rates achieved with 1PN blastocysts in 1PN embryos did not significantly differ from the control group. Thirty-three transfer cycles with 33 blastocysts derived from 1PN embryos in cIVF resulted in nine deliveries with no newborn malformations; however, no implantation was observed in three ICSI cycles. Conclusion Although the blastocyst formation rate of 1PN embryos was significantly lower than 2PN embryos in cIVF and ICSI cycles, 1PN blastocysts in cIVF, and not from ICSI, demonstrated an adequate ongoing pregnancy rate. These results suggested that 1PN blastocysts in cIVF are available for clinical use and may lead to an increase in the chance of pregnancy in patients receiving assisted reproductive technology with 1PN embryos.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Itoi¹,

Asano²,

Shimizu³

et al. 2015

J Assist Reprod Genet

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“…Occasionally, only one pronucleus (1PN) is observed in oocytes. The frequencies of the occurrence of 1PN zygotes ranged from 1.6% to 7.7% [1]. The mechanism of 1PN zygotes has been extensively studied as: a) asynchrony of the pronuclear appearance [2][3][4] or b) the male and female pronuclear fusion together [5,6], which will result in chromosomally normal embryos; while c) androgenetic or gynogenetic parthenogenesis, which will lead to haploid embryos [7,8].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

Liao

Zhang

Cheng

et al. 2009

J Assist Reprod Genet

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Purpose By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture. Methods After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.Results The diploid rate of blastocysts was 74.6%, which was significantly (P<0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (P < 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs. Conclusions Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.

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“…Although the majority of 1PN zygotes are incapable of further development, a small number develop to the blastocyst stage. Moreover, they successfully progress to a live birth in cases where no other embryos exist of better quality [30][31][32] and despite the low pregnancy rate. Nevertheless, because of the uncertainty of their genetic normality, transferring such embryos is controversial among ART laboratories.…”

Section: Aberrant Behavior Of Female Pn (Fpn) During the Pronuclear Smentioning

confidence: 99%

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

Iwata

Mio²

2016

Reprod Medicine & Biology

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Assisted reproductive technology (ART) has yielded vast amounts of information and knowledge on human embryonic development in vitro; however, still images provide limited data on dynamic changes in the developing embryos. Using our high-resolution time-lapse cinematography (hR-TLC) system, we were able to describe normal human embryonic development continuously from the fertilization process to the hatched blastocyst stage in detail. Our hR-TLC observation also showed the embryonic abnormality of a third polar body (PB)-like substance likely containing a small pronucleus being extruded and resulting in single-pronucleus (1PN) formation, while our molecular biological investigations suggested the possibility that some 1PN embryos could be diploid, carrying both maternal and paternal genomes. Furthermore, in some embryos the extruded third PB-like substance was eventually re-absorbed into the ooplasm resulting in the formation of an uneven-sized, two-PN zygote. In addition, other hR-TLC observations showed that cytokinetic failure was correlated with equal-sized, multi-nucleated blastomeres that were also observed in the embryo showing early initiation of compaction. Assessment combining our hR-TLC with molecular biological techniques enables a better understanding of embryonic development and potential improvements in ART outcomes.

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Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Cited by 25 publications

References 15 publications

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

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