Monthly variations in some reproductive parameters of Barki and Awassi rams throughout 1 year under subtropical conditions 2. Biochemical and enzymatic properties of seminal plasma

Taha, Taha A.; Abdel-Gawad, Eman I.; Ayoub, Mohammed Akli

doi:10.1017/s135772980005517x

Cited by 19 publications

(9 citation statements)

References 14 publications

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“…Similarly, El-Nagar (2017) found the highest conception rate of Friesian cows artificially inseminated with semen extended with MOLE as compared to Tris egg-yolk. Such results were supported by the theory that, good quality of semen was characterized by lower AST, ALT and LDH of enzyme activity (Taha et al, 2000;Borah et al, 2015, El-Nagar, 2017. Generally, reducing activity of enzyme in seminal plasma of thawed semen is an indicator of membrane integrity, and subsequently sperm motility (El-Harairy et al, 2011)…”

Section: Discussionmentioning

confidence: 79%

Cryopreservation and Quality Assessment of Buffalo Bull (Bubalus bubalis) Semen Using New Moringa Extender and Antioxidant Co-q10

Doidar¹,

El-Nagar²,

Elrefy³

et al. 2018

Journal of Animal and Poultry Production

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Low fertility in buffaloes with frozen-thawed semen is attributed to sperm damage that is related to formation of ice crystals during freeze/thaw process. This study was conducted to evaluate the freezability of buffalo bull semen extended with four types of extenders, including citrate egg-yolk (CEY, control), CEY with ethanolic Moringa Olifera Leaf Extract (eMOLE) as replacement of antibiotics (CEYm), eMOLE and eMOLE with conventional antibiotic (eMOLEa) with or without Coenzyme Q10 as antioxidant. Semen was subjected to calculate progressive motility, livability, abnormality, acrosome status and integrity of sperm membrane in semen after extending, equilibrating and thawing processes. Four sexually mature buffalo bulls (4-5 y and 400-450 kg LBW) were used for semen collection, and semen was collected twice weekly for 4 successive weeks. Semen ejaculates of ≥70% as mass motility percentage were pooled and extended at 37°C by the four types of extenders without or with 30 µM Co-Q10). Results cleared that progressive motility, livability, abnormality, acrosomal damage and plasma membrane integrity percentages improved (P<0.05) in buffalo semen after dilution, equilibration and thawing, while activity of AST, ALT and LDH in seminal plasma of post-thawed semen reduced (P<0.05) by extension of semen with eMOLE as a new extender. All sperm parameters and enzyme activity were maintained by replacing antibiotics in CEY extender with 4 ml eMOLE. All sperm parameters were improved (P<0.05) and enzyme activity was maintained by adding 30 µM of coQ10 as compared to free extenders. Fertility rate of buffalo cows inseminated with semen cryopreserved in extender (eMOLE+coQ10) was the highest (91.6%), followed by eMOLE without coQ10 (83.3%) and the lowest for control CEY (66.6%). In conclusion, eMOLE as a new extender, is promising for the extension and preservation of buffalo semen. Also, adding 4 ml of eMOLE could be a good substitute for the antibiotic component of CEY as a conventional semen extender. Moreover, supplementing semen extenders with antioxidant addition (30 µM CoQ10) is important to improve function of spermatozoa and sperm fertility of cryopreserved semen of buffaloes.

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Section: Discussionmentioning

confidence: 79%

Cryopreservation and Quality Assessment of Buffalo Bull (Bubalus bubalis) Semen Using New Moringa Extender and Antioxidant Co-q10

Doidar¹,

El-Nagar²,

Elrefy³

et al. 2018

Journal of Animal and Poultry Production

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“…A glycerol complex, glyceryl‐phosphorylcholine was reported in SP (Dawson et al, 1956), and it was hypothesized that glyceryl‐phosphorylcholine might be an important source of energy for spermatozoa in the female reproductive tract (White and Wallace, 1961). Studies with ram semen have indicated that reduced sperm concentration and motility are associated with decreased SP lipid content (Taha et al, 2000). Total lipids and phospholipids have been quantified in alpaca SP (Garnica et al, 1993).…”

Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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“…Improving incidence of acrosomal damage in the present study was associated with the lower activity of AST, ALT and LDH due to CoQ10 enrichment to extender of both species, being better for buffalo than cattle semen. Such results were supported by the theory that, good quality of semen characterized by lower activity of AST, ALT and LDH enzymes (Taha et al, 2000;El-Harairy et al, 2011), that could be due to lower injuries inflicted to cell membrane as evidenced by significantly lower total incidence of acrosomal changes (Borah et al 2015).…”

Section: Discussionmentioning

confidence: 64%

Effect of Coenzyme Q10 as an Antioxidant Added to Semen Extender During Cryopreservation of Buffalo and Cattle Semen

Saeed

El-Nagar

Wafa

et al. 2016

Journal of Animal and Poultry Production

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This study was conducted to assess the influence of supplementing Tris-extender with Coenzyme Q10 (COQ10) as an antioxidant on sperm characteristics of Egyptian buffalo and cattle. This study was carried out at Animal Production Research Station, El-Gemmezah, belonging to Animal Production Research Institute, Egypt. Five sexually mature buffalo (4 y and 400-450 kg) and Friesian (3 y and 350-400 kg) bulls were used in this study. Semen was collected twice weekly for five successive weeks. Only ejaculates with ≥70% mass motility were pooled and diluted at 37°C with Tris-extender containing 0, 20 and 30 μM of CoQ10 in T1, T2 and T3, respectively. Equilibration period was at 5°C for 4 h and semen was packed in 0.25 ml French straws frozen in liquid nitrogen-196 o C. Stored straws were thawed individually at 37°C for 30 s. Semen was evaluated for sperm characteristics including percentages of progressive motility, livability, abnormality, curled tail and acrosomal damage of spermatozoa. Enzymatic activity of AST, ALT and LDH was determined in seminal plasma of post-thawed semen. Results showed that there were no species differences in all sperm characteristics studied, except for increasing (P<0.05) percentage of abnormality and damage acrosome spermatozoa in post-thawed semen of cattle compared with buffalo bulls. All sperm characteristics were improved (P<0.05) by 30 μM COQ10 supplementation T3 in post-diluted, post-equilibrated and post thawed semen as compared to control without supplementation T1 in buffalo and cattle semen. Activity of all enzymes reduced (P<0.05) in seminal plasma of both species. In conclusion, CoQ10, as antioxidant, at a level of 30 μM in Tris-extender is able to enhance most sperm characteristics including mobility, livability, plasma membrane integrity, with decreasing sperm abnormalities and it has strong protective power against acrosomal damage of buffalo and cattle spermatozoa.

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Monthly variations in some reproductive parameters of Barki and Awassi rams throughout 1 year under subtropical conditions 2. Biochemical and enzymatic properties of seminal plasma

Cited by 19 publications

References 14 publications

Cryopreservation and Quality Assessment of Buffalo Bull (Bubalus bubalis) Semen Using New Moringa Extender and Antioxidant Co-q10

Cryopreservation and Quality Assessment of Buffalo Bull (Bubalus bubalis) Semen Using New Moringa Extender and Antioxidant Co-q10

Seminal Plasma: An Essential Attribute to Spermatozoa

Effect of Coenzyme Q10 as an Antioxidant Added to Semen Extender During Cryopreservation of Buffalo and Cattle Semen

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