Moonlight-like proteins are actually cell wall components in Pseudocercospora fijiensis

Canto-Canché, Blondy; Burgos-Canul, Yamily Yazmin; Chi-Chuc, Deysi; Tzec-Simá, Miguel; Ku-González, Angela; Brito-Argáez, Ligia; Carrillo-Pech, Mildred; De los Santos-Briones, César; Canseco-Pérez, Miguel Ángel; Luna-Moreno, Donato; Beltrán-García, Miguel J.; Islas-Flores, Ignacio

doi:10.1007/s11274-023-03676-3

Cited by 1 publication

(4 citation statements)

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“…The linkage of cell wall proteins can be categorized based on the different extraction methods. ,, CWPs in F. graminearum demonstrate a diverse array of linkages, as detailed in Figures a,b, S7, and Table S4.…”

Section: Resultsmentioning

confidence: 99%

“…It is interesting that the CWPs involved in NCDB groups were enriched in GO terms related to protein synthesis, which typically occurs in the cytoplasm and endoplasmic reticulum. This could be attributed to “moonlighting” proteins with dual functionalities − or potential experimental artifacts during extraction, leading to inadvertent cytoplasmic protein contamination. , …”

Section: Resultsmentioning

confidence: 99%

“…CWPs have different functions ranging from maintaining cellular shape to synthesizing and reorganizing wall components . Their composition is also known to vary in response to environmental conditions and developmental stages. − CWPs can be categorized as “typical” or “classical”, those with a signal peptide or glycosylphosphatidylinositol (GPI) anchor domain, and “atypical” or “nonclassical”, which lack these features. − Although early studies considered atypical cell wall proteins as cytosolic contaminants, recent studies reveal that these atypical CWPs are not contaminants and perform bifunctional roles (“moonlighting” proteins). ,− Several cell wall proteomics studies from different fungal species spotlight the diverse linkages of proteins anchored to the wall. ,,, These linkages range from noncovalent bonds to covalent bonds such as disulfide bonds, GPI anchor, and alkali-sensitive linkage (ASL). These cell wall proteins with different types of linkages can be extracted by treating different chemical reagents.…”

Section: Introductionmentioning

confidence: 99%

“…10,12−15 Several cell wall proteomics studies from different fungal species spotlight the diverse linkages of proteins anchored to the wall. 9,14,16,17 These linkages range from noncovalent bonds to covalent bonds such as disulfide bonds, GPI anchor, and alkali-sensitive linkage (ASL). These cell wall proteins with different types of linkages can be extracted by treating different chemical reagents.…”

Section: ■ Introductionmentioning

confidence: 99%

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Proteomic Analysis of Cell Wall Proteins with Various Linkages in Fusarium graminearum

Moon,

Min,

Winarto

et al. 2024

J. Agric. Food Chem.

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The fungal cell wall, primarily comprising a glucan−chitin matrix and cell wall proteins (CWPs), serves as a key mediator for fungal interactions with the environment and plays a pivotal role in virulence. In this study, we employed a comprehensive proteomics approach to analyze the CWPs in the plant pathogenic fungus Fusarium graminearum. Our methodology successfully extracted and identified 1373 CWPs, highlighting their complex linkages, including noncovalent bonds, disulfide bridges, alkali-sensitive linkages, and glycosylphosphatidylinositol (GPI) anchors. A significant subset of these proteins, enriched in Gene Ontology terms, suggest multifunctional roles of CWPs. Through the integration of transcriptomic and proteomic data, we observed differential expression patterns of CWPs across developmental stages. Specifically, we focused on two genes, Fca7 and Cpd1, which were upregulated in planta, and confirmed their localization predominantly outside the plasma membrane, primarily in the cell wall and periplasmic space. The disruption of FCA7 reduced virulence on wheat, aligning with previous findings and underscoring its significance. Overall, our findings offer a comprehensive proteomic profile of CWPs in F. graminearum, laying the groundwork for a deeper understanding of their roles in the development and interactions with host plants.

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