More on seed longevity phenotyping

Hay, Fiona R.; Davies, Rachael M.; Dickie, John B.; Merritt, David J.; Wolkis, Dustin

doi:10.1017/s0960258522000034

Cited by 20 publications

(14 citation statements)

References 35 publications

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“…The conditions were EPPO storage (200 times higher oxygen levels, 35°C and theoretically at 50% RH), ambient pressure (air) conditions mimicking medium-term seed storage under dry conditions in controlled warehouses (CWSS, seed equilibrated to 40% RH and 28°C), short-term traditional storage practice (TRSS, seeds equilibrated to 60% RH and 28°C) and CD storage (seeds equilibrated to 75% RH and 35°C). The duration of storage in which the seed lot viability declines to 50% ( P 50 ) is considered a suitable measure of seed longevity ( Hay et al., 2022a ). Based on the P 50 values ( Supplementary Table 4 ), it was observed that seeds from all lots lost their viability in shorter times under dry-EPPO storage or wet-CD storage compared to storage under the two ambient storage conditions (CWSS and TRSS).…”

Section: Discussionmentioning

confidence: 99%

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Kodde

Angenent

et al. 2022

Front. Plant Sci.

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Seed aging during storage results in loss of vigor and germination ability due to the accumulation of damage by oxidation reactions. Experimental aging tests, for instance to study genetic variation, aim to mimic natural aging in a shorter timeframe. As the oxidation rate is increased by elevating the temperature, moisture, and oxygen levels, this study aimed to (1) investigate the effect of experimental rice seed aging by an elevated partial pressure of oxygen (EPPO), (2) elucidate the mechanism of dry-EPPO aging and (3) compare aging under dry-EPPO conditions to aging under traditional moist-controlled deterioration (CD) conditions and to long-term ambient storage. Dry seeds from 20 diverse rice accessions were experimentally aged under EPPO (200 times higher oxygen levels), at 50% relative humidity (RH), along with storage under high-pressure nitrogen gas and ambient conditions as controls. While no decline in germination was observed with ambient storage, there was significant aging of the rice seeds under EPPO storage, with considerable variation in the aging rate among the accessions, with an average decline toward 50% survival obtained after around 21 days in EPPO storage and total loss of germination after 56 days. Storage under high-pressure nitrogen gas resulted in a small but significant decline, by an average of 5% germination after 56 days. In a second experiment, seven rice seed lots were stored under EPPO as compared to a moist-CD test and two different long-term ambient storage conditions, i.e., conditioned warehouse seed storage (CWSS) and traditional rice seed storage (TRSS). Untargeted metabolomics (with identification of lipid and volatile compounds profiles) showed a relatively high increase in levels of oxidized lipids and related volatiles under all four storage conditions. These compounds had a high negative correlation with seed viability, indicating oxidation as a main deteriorating process during seed aging. Correlation analysis indicated that EPPO storage at 50% RH is more related to aging under TRSS at 60% and CD-aging at 75% ERH rather than CWSS at 40% ERH. In conclusion, aging rice seeds under EPPO conditions is a suitable experimental aging method for analyzing variation among seed lots or genotypes for longevity under storage.

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Section: Discussionmentioning

confidence: 99%

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Kodde

Angenent

et al. 2022

Front. Plant Sci.

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“…The lower the oil content, the greater the amount of water that will be adsorbed, as seen for example for seeds of Camellia oleifera with differing oil content (Zhu et al, 2021 ). This is a key reason why equilibrating seeds so they have the same eRH can be considered preferable to equilibrating seeds to the same moisture content when comparing physiological responses such as seed longevity across diverse species (Hay et al, 2022 ). It also explains why different tissues within a seed can have different moisture contents.…”

Section: Influence Of Composition Temperature and Moisture History On...mentioning

confidence: 99%

“…However, it is perhaps more common for pre-dried seeds to be used. Seed storage experiments, for example, tend to be conducted for seeds that are adsorbing, since the moisture content is normally increased so that loss of viability can be followed over a reasonable length of time (Ellis and Roberts, 1980a , b ; Newton et al, 2014 ; De Vitis et al, 2020 ; Hay et al, 2022 ). According to Rahman and Labuza ( 2007 ), “ the desorption hysteresis loop usually ends at the monolayer ” or, in other words, that it is necessary to dry below M m for the material to cross over to the adsorption isotherm when water is added.…”

Section: Influence Of Composition Temperature and Moisture History On...mentioning

confidence: 99%

“…This recommendation came from cross-referencing estimates of the low moisture content limit to the applicability of the viability equations to sorption isotherms (Ellis et al, 1989 , 1992 ), although it was subsequently accepted that the RH where the low critical moisture content occurred increased as the storage temperature decreased (e.g., from 10.4–10.8 at 65°C to 13.1–13.5 at 39.9°C in the case of Trifolium pratense and Medicago sativa ; Ellis and Hong, 2006 ). Because then, there is a clear RH range over which the viability equations are applicable, and since seeds are normally stored within this range (i.e., “air-dry”), for studies of comparative longevity of seeds of different taxa it makes sense that seeds are also stored at an equilibrium RH within this same range (Hay et al, 2019 ); more precisely, an RH of 60% has been recommended (Newton et al, 2014 ; Hay et al, 2022 ) as this RH is sufficiently high for the aging process to be accelerated such that viability loss can be followed over days–months, depending on the storage temperature, but not so high that enzymic reactions occur at a significant rate. Indeed, a number of papers have reported different relative rates of aging of seed lots at ≥75% RH (controlled deterioration conditions) cf .…”

Section: Isotherms In Seed Science: Informing Longevity Dormancy and ...mentioning

confidence: 99%

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Seed Moisture Isotherms, Sorption Models, and Longevity

2022

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Seed moisture sorption isotherms show the equilibrium relationship between water content and equilibrium relative humidity (eRH) when seeds are either losing water from a hydrated state (desorption isotherm) or gaining water from a dry state (adsorption isotherm). They have been used in food science to predict the stability of different products and to optimize drying and/or processing. Isotherms have also been applied to understand the physiological processes occurring in viable seeds and how sorption properties differ in relation to, for example, developmental maturity, degree of desiccation tolerance, or dormancy status. In this review, we describe how sorption isotherms can help us understand how the longevity of viable seeds depends upon how they are dried and the conditions under which they are stored. We describe different ways in which isotherms can be determined, how the data are modeled using various theoretical and non-theoretical equations, and how they can be interpreted in relation to storage stability.

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“…2). Results from comparative longevity protocols are expected to be meaningful in understanding seed longevity in genebanks (Hay et al 2022), but still, little is known about the relationship between longevity after controlled deterioration tests and genebank storage due to the lack of comparative studies between the two environments (but see Gianella et al 2022). In our study, longevity differences between accessions estimated through experimental ageing conditions did not depend on the timing of genebank storage, suggesting that seed viability of several (short-lived) alpine species can be safety retained for at least 20 years.…”

Section: Years Of Genebank Storage Does Not Affect Seed Longevitymentioning

confidence: 99%

Two decades of climate change alters seed longevity in an alpine herb: implications for ex situ seed conservation

et al. 2022

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Climate warming in mountain areas is increasing faster than the global average, threatening alpine plants. Climate affects many traits including seeds, the longevity of which is important for conservation, facilitating genebank storage. Seeds of alpine species are considered short-lived in storage, but their longevity increases when produced under a warmer parental environment. Consequently, with climate warming, seeds of alpine species may have increased fitness and be longer-lived in genebank storage. We assessed seed longevity under artificial ageing in 10 accessions of the arctic-alpine species Viscaria alpina stored under genebank conditions for different time periods over the last 20 years. The seed collection site was in the northern Apennines, where above average warming and variable precipitation has been recorded. The time taken for viability to fall to 50% (p50) was estimated using probit analysis; correlation and general linear regression were used to investigate the effects of length of time in storage, seed mass and climate under which seeds were produced on seed longevity. p50 varied between 7.77 and 18.49 days. There was no relationship between length of time in storage or seed mass on seed longevity. p50 was higher in years with increased temperature and lower precipitation during the growing season, with precipitation having more impact than temperature. The results suggest that seeds of alpine species are suitable for genebank storage, and inter-annual variation in precipitation induces a plastic response in seed longevity. Using genebank stored seeds provides insights into how alpine species may respond to future climate changes and could have implications for genebank storage.

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More on seed longevity phenotyping

Cited by 20 publications

References 35 publications

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Seed Moisture Isotherms, Sorption Models, and Longevity

Two decades of climate change alters seed longevity in an alpine herb: implications for ex situ seed conservation

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