Morfogênese do testículo de embriões e fetos de vacas da raça nelore (Bos taurus indicus)

Jacomini, José Octávio; Esper, César Roberto; Diniz, Elmo Gomes; Vieira, Ricardo; Beletti, Marcelo Emílio

doi:10.11606/issn.1678-4456.bjvras.2007.26658

Cited by 2 publications

(8 citation statements)

References 9 publications

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“…In this work, the germ cells were in the seminiferous tubules, and large cells with lightly stained, rounded nuclei, as previously reported (JACOMINI et al, 2007), are formed from 31 days of gestation (WROBEL; SUB, 1998). It was observed that after the fifth month of pregnancy, the number of these cells increased according to age (p<0.0001; Figure 1).…”

Section: Resultssupporting

confidence: 72%

“…This number increases to 1.5 billion by 10 years of age and is estimated at 3.7 billion cells in human adults (CORTES et al, 1987). During pregnancy, some variation in the number of Sertoli cells in the testes of Nellore bovine fetuses was described (JACOMINI et al, 2007). The Sertoli cells are the first somatic cells to differentiate in the male gonad, and they act as central regulators of testis organogenesis (SVINGEN; KOOPMAN, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…After inclusion, serial 3-μm-thick sections were created using the Leica rotary microtome (RM1255 model). Histological slides were mounted with a mean interval of 210 μm between cuts and were stained with hematoxylineosin (HE) (JACOMINI et al, 2007). After these procedures, the germ cells, Sertoli cells and Leydig cells in the testes were counted.…”

Section: Histological Processing Of the Fetal Testismentioning

confidence: 99%

“…The Sertoli, Leydig, and germ cells were classified according to previously described characteristics (JUNQUEIRA, CARNEIRO 2004;JACOMINI et al, 2007).…”

Section: Germ Sertoli and Leydig Cells: Cell Identification And Quanmentioning

confidence: 99%

“…To avoid count duplication of the same cell in more than one section and to correct for the number of sections in which a cell could be included, the cell diameter was divided by the thickness of the cut, and the total number of cell types counted was divided by this value. As a result, the total number of fetal testis cells was obtained (JACOMINI et al, 2007), and the nucleus diameter of each cell type was used as a reference for determining the quantity of testicular cells.…”

Section: Germ Sertoli and Leydig Cells: Cell Identification And Quanmentioning

confidence: 99%

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Bovine fetal testicular development in the Nellore breed

Souza

Miguel

Maioli

et al. 2017

SCA

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The study of gonadal development improves the understanding of factors that can influence the reproductive development process. This study aims to characterize bovine fetal testicular development and the testosterone level in the Nellore breed. For the study, 162 bovine fetuses aged between 3 and 8 months were collected from Nellore cows at a local abattoir. The fetal age was estimated by DP=8.4+0.087L+5.46√L, where DP is the estimated pregnancy day and L represents fetal length. The fetal gonadal weight (g), width (cm), and thickness (cm) were measured. Thereafter, the gonads were submitted to classic histology processes in 3-µm-thick slices cut at 210 µm intervals. The Sertoli cells, Leydig cells, and germ cells were counted. Blood samples were collected from umbilical cords for testosterone levels. The data were analyzed using the Spearman correlation test followed by Principal Component Analysis and one-way ANOVA to compare the averages between months. The testicular weight and volume were found to have a positive correlation with the numbers of Sertoli cells (r = 0.84; p < 0.0001 and r = 0.92; p < 0.0001, respectively), Leydig cells (r = 0.80; p < 0.0001 and r = 0.90; p < 0.0001, respectively), and germ cells (r = 0.84; p < 0.0001 and r = 0.93; p < 0.0001, respectively) and to be negatively correlated with testosterone plasmatic concentration (r = -0.31; p = 0.0001 and r = -0.22; p = 0.006, respectively) during pregnancy. After the fifth month, the numbers of Sertoli cells, Leydig cells and germ cells differed (p < 0.0001) from the following gestational months. The highest testosterone concentration (p = 0.007) was observed in the fifth month of gestation and was followed by a concentration decrease in the seventh and eighth months. The increase in cell quantity was responsible for the increase in testicular weight and volume during fetal development. On the other hand, the testosterone concentration followed the increase in testicular weight and volume until the 7 th month of gestation and regressed during the 8 th and 9 th months, in addition to the increase in cell number. as mensurações, as gônadas foram submetidas ao processamento histológico clássico, sendo utilizadas para elaboração das lâminas cortes com 3 µm de espessura com intervalos entre cortes de 210 µm. As células de Sertoli, Leydig e germinativas foram contadas. Amostras de sangue foram coletadas do cordão umbilical para quantificação dos níveis de testosterona. Os dados foram analisados utilizando a correlação de Spearman seguida pela análise de componentes principais, sendo o comparativo de médias entre os meses realizados utilizando o one-way ANOVA. O peso e o volume testicular apresentaram correlação positiva com o número de células de Sertoli (r = 0,84; p < 0,0001 e r = 0,92; p < 0,0001, respectivamente), células de Leydig (r = 0,80; p < 0,0001 e r = 0,90; p < 0,0001, respectivamente), e células germinativas (r = 0,84; p < 0.0001 e r = 0,93; p < 0,0001, respectivamente), porém foram negativamente correlacionados com a concentraç...

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Histological Processing Of the Fetal Testismentioning

confidence: 99%

“…The Sertoli, Leydig, and germ cells were classified according to previously described characteristics (JUNQUEIRA, CARNEIRO 2004;JACOMINI et al, 2007).…”

Section: Germ Sertoli and Leydig Cells: Cell Identification And Quanmentioning

confidence: 99%

Section: Germ Sertoli and Leydig Cells: Cell Identification And Quanmentioning

confidence: 99%

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Bovine fetal testicular development in the Nellore breed

Souza

Miguel

Maioli

et al. 2017

SCA

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Início da puberdade de cordeiros provenientes de ovelhas deslanadas submetidas à restrição calórica ou proteica

Fraga

Hatamoto-Zervoudakis

Júnior

et al. 2015

Rev. bras. saúde prod. anim.

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ResumoO estudo foi realizado para avaliar se a restrição calórica ou proteica em fêmeas lactantes influencia o início da puberdade de seus cordeiros. Foram utilizados 21 cordeiros, sendo duas fêmeas e cinco machos por tratamento. Durante sessenta dias as mães receberam o tratamento aleatoriamente escolhido para o seu cordeiro: controle (TC, mantença), restrição calórica (TRC, ½ da energia do TC) e restrição proteica (TRP, 1/3 da proteína do TC). Após o desmame, os cordeiros receberam uma dieta composta por silagem de milho e concentrado, sendo pesados e avaliados quinzenalmente. Nos machos, coletava-se o ejaculado, mensurava-se circunferência escrotal e as dimensões testiculares. Nas fêmeas, realizava-se ultrassonografia para medir os ovários e verificar presença de corpo lúteo. Os machos eram considerados púberes se apresentassem ejaculado com, no mínimo: motilidade de 50%, concentração do ejaculado de 50 x 106espermatozóides/mL e, no máximo, 50% de anormalidades espermáticas totais, e as fêmeas, se apresentassem corpo lúteo. Foi encontrada interação entre período de avaliação e tratamento para circunferência escrotal (p=0,0019), volume testicular médio (p=0,0025), motilidade (p<0,0001) e concentração espermática (p=0,027), sendo o TRP, mais tardio do que TRC e TC em todas variáveis. Nas borregas não houve diferença (p>0,05) entre os tratamentos quanto às características foliculares, porém houve quanto ao corpo lúteo (p=0,0179). Notou-se que as fêmeas do TC apresentaram corpo lúteo a partir da 10a semana, TRC da 11a e o TRP da 13a. Concluiu-se que a restrição proteica materna durante a lactação atrasa o início da puberdade de seus cordeiros.

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Morfogênese do testículo de embriões e fetos de vacas da raça nelore (Bos taurus indicus)

Cited by 2 publications

References 9 publications

Bovine fetal testicular development in the Nellore breed

Bovine fetal testicular development in the Nellore breed

Início da puberdade de cordeiros provenientes de ovelhas deslanadas submetidas à restrição calórica ou proteica

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