“…Anatomical fresh sections were made with a hand microtome (Allmikro-Mikrotom, N€ urnberg, Germany); the specimens were held between two pieces of polystyrene foam and then were secured inside the microtome device without the need to embed the axes. Fresh sections were immediately examined using fluorescence microscopy with a Leica Jones, 1905;Stokey, 1907;Hill, 1914;Pixley, 1968;Wilder, 1970;Chu, 1974;Warmbrodt & Evert, 1974a,b;Øllgaard, 1975, 1979, 1987, 2015Bruce, 1976;Stevenson, 1976;Gola et al, 2007;Carlquist et al, 2012;Arana et al, 2014;Rinc on-Baron et al, 2014;Lopes et al, 2020).…”