Mori Cortex Radicis extract inhibits human norovirus surrogate in simulated digestive conditions

Lim, Chae Yeon; Kim, Hyojin; Chung, Mi Sook

doi:10.1007/s10068-021-00958-0

Cited by 2 publications

(3 citation statements)

References 32 publications

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“…[ 22 ] The active compounds found in Mori Cortex, such as Moracin A and Morusin, endow it with distinct pharmacological characteristics, highlighting its significance in TCM treatments. [ 23 ] Therefore, this study explores the anti-OS mechanism of Mori Cortex and its main active component, Morusin, using network pharmacology and in vitro experimental methods.…”

Section: Discussionmentioning

confidence: 99%

Investigating the molecular mechanism of Mori Cortex against osteosarcoma by bioinformatics analysis and in vitro experimental

Wang,

Xie

et al. 2024

Medicine

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Objective: To explore the therapeutic mechanism of Mori Cortex against osteosarcoma (OS), we conducted bioinformatics prediction followed by in vitro experimental validation. Methods: Gene expression data from normal and OS tissues were obtained from the GEO database and underwent differential analysis. Active Mori Cortex components and target genes were extracted from the Traditional Chinese Medicine System Pharmacology database. By intersecting these targets with differentially expressed genes in OS, we identified potential drug action targets. Using the STRING database, a protein-protein interaction network was constructed. Subsequent analyses of these intersected genes, including Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment, were performed using R software to elucidate biological processes, molecular functions, and cellular components, resulting in the simulation of signaling pathways. Molecular docking assessed the binding capacity of small molecules to signaling pathway targets. In vitro validations were conducted on U-2 OS cells. The CCK8 assay was used to determine drug-induced cytotoxicity in OS cells, and Western Blotting was employed to validate the expression of AKT, extracellular signal-regulated kinases (ERK), Survivin, and Cyclin D1 proteins. Results: Through differential gene expression analysis between normal and OS tissues, we identified 12,364 differentially expressed genes. From the TCSMP database, 39 active components and 185 therapeutic targets related to OS were derived. The protein-protein interaction network indicated that AKT1, IL-6, JUN, VEGFA, and CASP3 might be central targets of Mori Cortex for OS. Molecular docking revealed that the active compound Morusin in Mori Cortex exhibits strong binding affinity to AKT and ERK. The CCK8 assay showed that Morusin significantly inhibits the viability of U-2 OS cells. Western Blot demonstrated a reduction in the p-AKT/AKT ratio, the p-ERK/ERK ratio, Survivin, and Cyclin D1. Conclusion: Mori Cortex may exert its therapeutic effects on OS through multiple cellular signaling pathways. Morusin, the active component of Mori Cortex, can inhibit cell cycle regulation and promote cell death in OS cells by targeting AKT/ERK pathway.

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Section: Discussionmentioning

confidence: 99%

Investigating the molecular mechanism of Mori Cortex against osteosarcoma by bioinformatics analysis and in vitro experimental

Wang,

Xie

et al. 2024

Medicine

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“…Many natural products with promising antiviral activities in vitro display reduced activity against NV under gastrointestinal conditions [ 33 , 34 ]. Mori Cortex Radicis, aged-green tea extract, and blueberry proanthocyanidins also reduce MNV titers under simulated conditions [ 21 , 33 , 34 ]. It has been reported that MNV capsid proteins are sensitive to the presence of bile, Mg 2+ , and Ca 2+ , and that at low pH—at which they are induced to contract between the P and the S domains—they enhance receptor binding [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…MNV-1 was kindly provided by Dr. Herbert Virgin (Vir Biotechnology, San Francisco, CA, USA). MNV was replicated and purified as previously reported [ 21 ]. The purified MNV suspension was stored as aliquots at −80 °C until its use.…”

Section: Methodsmentioning

confidence: 99%

Antiviral Activities of Asarones and Rhizomes of Acorus gramineus on Murine Norovirus

Kim

Maeng

Lee

et al. 2022

Viruses

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Noroviruses (NVs) are a major cause of foodborne diseases worldwide. The rhizomes of Acorus gramineus (AGR) have been used as a traditional medicinal plant and a food additive. In this study, AGR and its bioactive components—α-asarone and β-asarone—showed significant antiviral activities against murine NV (MNV) with pre-treatment, with more than two log reductions in viral plaques. They also demonstrated strong inhibition on binding to A- and O-type saliva by the recombinant P domain derived from human NV (HuNV) GII.4. Both α- and β-asarones also inhibited the binding of the P domain to the receptor at 0.125–1 mM in a concentration-dependent manner and induced a marked reduction in Tm, suggesting that they may reduce structural stability and block receptor binding by the P domain. In simulated digestive conditions, the AGR extract, α-asarone, or β-asarone further showed a significant reduction of MNV plaques by 1.5–2.8 logs. The asarones show a potential for development as a scaffold for anti-NV agents.

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Mori Cortex Radicis extract inhibits human norovirus surrogate in simulated digestive conditions

Cited by 2 publications

References 32 publications

Investigating the molecular mechanism of Mori Cortex against osteosarcoma by bioinformatics analysis and in vitro experimental

Investigating the molecular mechanism of Mori Cortex against osteosarcoma by bioinformatics analysis and in vitro experimental

Antiviral Activities of Asarones and Rhizomes of Acorus gramineus on Murine Norovirus

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