Moringa Oleifera Leaf Extract Loaded Hydrogel for Diabetic Wound Healing

Abstract

Diabetic wounds (DW) are a chronic, non-healing wound on the feet of diabetic patients that pose a serious challenge to world health. Around 84% of diabetic patients undergo lower leg amputations. Though numerous topical and systemic drugs have been used to heal the DW, these drugs have led to the emergence and subsequent rapid overgrowth of resistant bacterial strains, side effects and toxicity. Many herbal plants have very important role in wound healing because they promote the natural repair mechanisms. Moringaoleifera (MO) is an important medicinal plant which has an impressive range of medicinal uses including antimicrobial, anti-inflammatory, antidiabetic, antioxidant and anticancer activities. Recently few researchers reported that MO extracts have effective wound healing property due to the presence of rich flavonoids and vicenin-2. The objective of the present study was to develop hydrogel formulations loaded with Moringaoleifera leaves extract. The prepared hydrogels were evaluated for physical appearance, rheological behavior, skin irritation and wound-healing power in streptozotocin-induced diabetic male wistar albino rats. Results showed that all hydrogel formulations exhibited good and acceptable physical properties. All the animals tolerated the applied gels and no signs of irritations were noticed during the skin irritation study. The in-vivo wound healing studies showed a time dependent increase in percentage of wound, a contraction which is higher than that produced by the control groups. These contractions were statistically significant (P<0.001), during the first 10 days of the study with MO-Hydrogel administration. The MO-hydrogel showed the highest percent wound contraction with complete wound closure and epithelization was observed on 7thday of wound induction.

Scoping Review: Evaluation of Moringa oleifera (Lam.) for Potential Wound Healing in In Vivo Studies

“…Our search from the six databases produced a total of 80 related articles. After removing duplicates, 59 articles were screened and 18 articles were included [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ], as summarized in Figure 1 .…”

“…Majority of the studies indicate that M. oleifera was applied topically and formulated as gel (six studies [ 30 , 39 , 44 , 47 ] followed by using the extract in the form of paste, patch, or film dressing (five studies [ 30 , 33 , 36 , 40 ] and the remaining an ointment (three studies [ 34 , 41 , 42 ]. Two studies reported oral administration of M. oleifera aqueous extracts (two studies [ 38 , 43 ] while another two reported dual administration of both topical (ointment and aqueous extract) and oral (aqueous extracts) (two studies [ 32 , 37 ].…”

“…No abnormalities and no significant differences ( p < 0.05) were detected on body-weight-gain percentage [ 33 ]. Skin irritation studies of aqueous extracts of M. oleifera leaves hydrogel (500 mg/animal) administered topically on excision wound of Wistar rats (200–250 g) twice a day for 7 days showed no skin irritation signs during the whole period of study [ 47 ]. A skin irritation test carried out using a mixture of aqueous extracts of M. oleifera leaves (MO) and human amniotic membrane (AM)-formulated gel (2% MO + AM) administered topically on wounds on female Wistar rats for 7 days did not show any skin oedema, itchiness, or erythema, suggesting tolerable dermal application [ 39 ].…”

“…Wound healing studies have also always been associated with antidiabetic activity. M. oleifera showed an antidiabetic effect while accelerating wound healing on diabetic-induced animals [ 32 , 33 , 34 , 47 ]. The compounds present in M. oleifera , such as gallic acid, rutin, and vicenin-2 active compounds, as well as other flavonoids and phenolic metabolites, improved the hyperglycemic condition of diabetic-induced animal models [ 34 , 80 ].…”

Review on Diabetic Foot Ulcers and Its Herbal Approach to Treatment

“…A study conducted on a hyperglycemic animal model utilizing the leaves of M. oleifera (Moringaceae family) showed specific characteristics such as modulation of inflammatory mediators, improved wound contraction, and epithelialization [36]. Table 2 contains detailed properties of M. oleifera in treating diabetic foot ulcers.…”