Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin

Mardatillah, Meidy; Wurlina, Wurlina; Yudaniayanti, Ira Sari; Primarizky, Hardany; Plumeriastuti, Hani; Hamid, Iwan Sahrial

doi:10.20473/ovz.v11i1.2022.15-21

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…The presence of antioxidants in moringa leaves like flavonoids, vitamin C, vitamin E and quercetin is responsible for its ameliorative effect. 29 Amelioration by moringa herb is the topic of discussion in medical science these days but its effect on fluoxetine was not addressed before. So, this study is providing a track to explore it further.…”

Section: Discussionmentioning

confidence: 99%

Moringa Oleifera mitigates the Fluoxetine-induced damage to the histological architecture of the seminiferous tubules in adult male rats

Ayesha Ali,

Abdullah Qamar,

Maimoona Khan

et al. 2024

Ann Pak Inst Med Sci

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Objective: To observe the ameliorative changes of Moringa oleifera on the gross and histological architecture of fluoxetine-treated adult rat testis. Methodology: This experimental study was conducted in Department of Anatomy, Army Medical College, Rawalpindi in collaboration with the National Institute of Health (NIH), Islamabad and Pathology Lab Pak Emirates Military Hospital Rawalpindi, Pakistan, from October 22 to April 2023. Thirty male Sprague Dawley rats were randomly divided into three groups (n=10). Rats were kept at the animal house of NIH Islamabad. Group A (control), Groups B and C (experimental) were given doses dissolved in distilled water through oral gavage once daily. The experimental period was 08 weeks. Group B was administered fluoxetine in a dose of 10 mg/kg body weight. Group C rats were treated with two chemicals, same dose of fluoxetine as in group B and concomitantly received 50 mg/30 grams body weight of Moringa Oleifera. After dissection, testes were fixed and staining was done with Hematoxylin & Eosin for histological study. Analysis of data was done on SPSS version 22. A p-value ? 0.05 was considered statistically significant. Results: The present study concluded that amelioration caused by Moringa Oleifera was statistically significant (p-value ?0.05) when compared to the other groups. Conclusion: Moringa Oleifera has ameliorative effects on the testicular toxicity induced by Fluoxetine in male rats by showing improvement in the diameter of the seminiferous tubules.

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Section: Discussionmentioning

confidence: 99%

Moringa Oleifera mitigates the Fluoxetine-induced damage to the histological architecture of the seminiferous tubules in adult male rats

Ayesha Ali,

Abdullah Qamar,

Maimoona Khan

et al. 2024

Ann Pak Inst Med Sci

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“…Lipid peroxidation is triggered by oxidative stress conditions, where there is an imbalance between ROS and the body's antioxidants. In addition to spermatogenic cells, ROS also causes degeneration in Leydig and Sertoli cells so that it will lower testosterone levels and affect the function of testicular spermatogenesis [11][12][13][14] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Ethanolic Extract of Moringa (Moringa oleifera, Lam.) Leaf on Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Model of Metabolic Syndrome

Devitasari,

Pesik,

Subandono

et al. 2023

SMedJour

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Introduction: Metabolic syndrome define as symptoms of body abnormalities that increased reactive oxygen species and cause disturbances of the germ cell layer thickness in seminiferous tubules. The content of Moringa oleifera has anti-diabetic, anti-dyslipidemia, and hypotensive effects. This study aimed to find out the effect of ethanolic extract of moringa leaves (Moringa oleifera, Lam.) on the histopathology of seminiferous tubules of Wistar rats (Rattus norvegicus) induced metabolic syndrome with Streptozotocin-Nicotinamide and high-fat diet.Methods: This research was experimental post-only control group design. The subjects were 30 male Rattus norvegicus grouped into normal group (K1), metabolic syndrome group (K2), and 3 metabolic syndrome groups with ethanolic extract of moringa leaf at doses of 150 mg/kg (K3), 250 mg/kg (K4) and 350 mg/kg of body weight (K5).Results: Thickness of tubules (µm) of group K1 (107.48±10.74), K2 (53.87±25.09), K3 (80.52±3.50), K4 (81.94±9.40), K5 (94.04±8.66). Average number of cell layers of group K1 (8.00±1.09), K2 (3.65±1.35), K3 (5.99±0.36), K4 (6.57±0.36), K5 (6.72±0.20). There was a significant difference in tubule wall thickness and the number of cell layers between the five groups (p<0.05). The K3, K4, and K5 differed significantly from the K2 (p<0.05). K5 has value closest to K1. Conclusion: Ethanolic extract of moringa (Moringa oleifera, Lam.) leaf increased the wall thickness and number of cell layers of seminiferous tubules of Wistar rats (Rattus norvegicus) model of metabolic syndrome with ethanolic extract of moringa leaf at doses of 350 mg/kg of body weight most effectively improved the histopathological of the seminiferous tubules.

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“…Maceration was carried out for 3 × 24 hours and stirred every day, then filtered to obtain the form of macerate. The macerate was put into the rotary evaporator at 40°C with a speed of 50 rpm until all the solvent and water content of the Moringa leaves were separated so that a concentrated ethanol extract of Moringa leaves was obtained (Mardatillah et al, 2022).…”

Section: Extraction Of Moringa Leavesmentioning

confidence: 99%

Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats

Ngizzah,

Wurlina Wurlina,

Poedji Hastutiek

et al. 2023

Ovz

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This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.

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Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin

Cited by 11 publications

References 34 publications

Moringa Oleifera mitigates the Fluoxetine-induced damage to the histological architecture of the seminiferous tubules in adult male rats

Moringa Oleifera mitigates the Fluoxetine-induced damage to the histological architecture of the seminiferous tubules in adult male rats

Effect of Ethanolic Extract of Moringa (Moringa oleifera, Lam.) Leaf on Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Model of Metabolic Syndrome

Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats

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