Morpho-Molecular Characterization and Virulence Determination of Entomopathogenic Fungal Isolates Native to Indian Subcontinent

Mishra, Sapna

doi:10.21767/2471-8521.100003

Cited by 4 publications

(5 citation statements)

References 33 publications

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“…The endophytic and epiphytic characteristics of EPF induce plant resistance to insect pests (Klieber and Reineke, 2016;Ramakuwela et al, 2020) and microbial disease-causing agents by increasing plant defense responses (Moonjely et al, 2016). Consequently, they have attracted attention as microbial insecticides to control insect pests because of their high virulence, broad host range and can be isolated from wide ranges of soil habitats particularly cultivated and forest soils existence in a wide range of habitats (Gürlek et al, 2018;Mishra et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Gebremariam

Chekol

Assefa

2021

Heliyon

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Fungal entomopathogens are the most effective biocontrol agents against insect pests in the natural ecosystem. This study was conducted for phenotypic, molecular, and virulence characterization of locally isolated entomopathogenic fungi from soil samples of six localities in Ethiopia. Entomopathogenic fungi were isolated from 120 soil samples with the galleria baiting method. A total of 65 (54.2%) entomopathogenic fungal isolates belongs to Beauveria spp and Metarhizium spp were identified based on cultural and morphological features. All isolates were pre-screened based on germination, vegetative growth rate, and spore production as fungal in-vitro virulence determinates. Isolate AAUKB-11 displayed the peak germination of 99.67% and isolate AAUMFB-77 achieved the highest radial growth rate of 3.43 mm day À1 with the highest sporulation 4.60 Â 10 8 spores/ml. The phylogenetic analysis of ITS-rDNA confirmed that 7 isolates were identified as B. bassiana and 5 isolates were categorized into M. anisopliae. Selected B. bassiana and M. anisopliae strains were evaluated for their pathogenicity efficiency against G. mellonella larvae and caused 86.67%-100% mortality. The mortality rates of G. mellonella larvae peaked at 100% with 4(33.33%) isolates from B. bassiana and 2(16.67%) isolates from M. anisopliae after 10 days of treatments. The high virulent isolate, B. bassiana AAUMB-29 displayed the least LT 50 value of 2.36 days followed by isolate B. bassiana AAUMFB-77 with LT 50 of 2.53 days. Future studies should be needed to focus on the evaluation of high virulent isolates against other potential insect pests to assess their vigorous role as favorable biological control agents.

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Section: Introductionmentioning

confidence: 99%

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Gebremariam

Chekol

Assefa

2021

Heliyon

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“…Beauveria bassiana HQ917687 used in this study for SSF was isolated from soil samples collected from Northern Uttar Pradesh, India (Mishra et al 2015). The fungal isolate was maintained on Potato Dextrose Agar slants at 4°C.…”

Section: Microorganismmentioning

confidence: 99%

Suitability of agricultural by-products as production medium for spore production by Beauveria bassiana HQ917687

Mishra

Kumar

Malik

2016

Int J Recycl Org Waste Agricult

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Purpose The seemingly harmful effect of chemical insecticides to the environment, plants and human health, has led to prominent role of entomopathogenic fungi (ENPF) as biopesticide. However, the success of ENPF as biocontrol agent depends on their amenability to easy and cheap mass multiplication. Solid state fermentation (SSF) has emerged as an appropriate technology for management of agro-industrial residues and for their value addition. In this study, the potentiality of agricultural by-products for mass multiplication of Beauveria bassiana was determined.Methods Two sets of SSF experiments with agricultural by-products [tea leaf waste, wheat bran, husk of rice, pigeon pea and urad, and seed cake of jatropha and pongamia] as substrates; one with fortification of nutrients, while other with no nutrient addition were performed. The substrates (200 g) were inoculated with B. bassiana spore suspension (1 9 10 8 spores/ml) and incubated at 28°C for 14 days. After the incubation period, B. bassiana spore concentrations were enumerated. Results Maximum spore production was observed in rice husk, while jatropha seed cake showed least spore production. Addition of nutrient supplements in the substrates showed slight (pongamia seed cake) to significant (rice husk, tea leaf waste) increase in spore production. The results showed constant increase in spore production with increase in C/N ratio of the substrates.Conclusions Mass production processes directly influences the cost, shelf life, virulence, and field efficacy of fungal pathogens. The results from this investigation are expected to pave the way towards commercialization of B. bassiana by augmenting the research in fungal mass production.

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“…Among the biological control agents, we can list entomopathogenic fungi (Mishra et al., 2015), bacteria (Lecuona et al., 2005), viruses (Lietze et al., 2013), parasitoid wasps (La Rossa et al., 2002), parasitic nematodes (Renn, 1998) and predators. Among the predators, mites, Histeridae beetles (Geden et al., 1988; Geden & Axtell, 1988) and other species of Muscidae, such as Hydrotaea aenescens (Wiedemann, 1830), are listed (Farkas et al., 1998; Hogsette et al., 2002; Hogsette & Washington, 1995; Müller, 1982; Schumann, 1982).…”

Section: Introductionmentioning

confidence: 99%

Controlling house fly populations under laboratory conditions: Hydrotaea aenescens larvae as effective predator

Cavalheiro,

Morales,

Madeira

et al. 2024

J Applied Entomology

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Larvae of Hydrotaea are facultative predators of larvae of flies commonly found in poultry farms, chicken and pig waste, and decaying carcasses. One species, the black dump fly, Hydrotaea aenescens (Wiedemann, 1830), coexists with the housefly Musca domestica (Linnaeus, 1758) in these environments and has been employed in the biological control of the housefly in the USA and Germany. To assess the predatory efficacy of H. aenescens larvae on M. domestica larvae, we conducted controlled laboratory experiments in which we selected larvae of different sizes of the larvae and the densities of both predator and prey. We set up the experiment using adult flies from a poultry farm and reared the larvae they produced in our laboratory. Our findings indicate that second‐ and third‐instar H. aenescens larvae effectively suppressed populations of housefly larvae under laboratory conditions, especially when the latter was smaller or the same size as the former. Under these ideal experimental conditions, a single H. aenescens larva consumed up to 29 larvae of M. domestica, potentially leading to a complete eradication of the housefly population at predator–prey density levels of 1:1 and 1:4, but only when considering both the natural mortality rate of M. domestica larvae and the additional mortality caused by H. aenescens larvae. These results corroborate a pattern previously observed in other predatory larvae and have important implications for biological control and integrated pest management programs targeting M. domestica in poultry and swine farms across South America.

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Morpho-Molecular Characterization and Virulence Determination of Entomopathogenic Fungal Isolates Native to Indian Subcontinent

Cited by 4 publications

References 33 publications

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Suitability of agricultural by-products as production medium for spore production by Beauveria bassiana HQ917687

Controlling house fly populations under laboratory conditions: Hydrotaea aenescens larvae as effective predator

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