Morpho-structural adaptations of the integument in different aquatic organisms

Alesci, Alessio; Pergolizzi, Simona; Mokhtar, Doaa M.; Fumia, Angelo; Aragona, Marialuisa; Lombardo, Giorgia Pia; Messina, Emmanuele; D’Angelo, Roberta; Cascio, Patrizia Lo; Sayed, Ramy K. A.; Albano, Marco; Capillo, Gioele; Lauriano, Eugenia Rita

doi:10.1016/j.acthis.2023.152031

Cited by 5 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…depilans, commonly named sea hare, is a gastropod mollusk in the Aplysiidae family. Its integument covers the entire soft tissue surface, including the head, foot, body, mantle, and external visceral surfaces [23]. The roles of the integument are to provide a protective barrier and facilitate respiration, locomotion, nutrient absorption, osmoregulation, the secretion of substances, and reproduction [24].…”

Section: Discussionmentioning

confidence: 99%

Functional Adaptations of Hemocytes of Aplysia depilans (Gmelin, 1791) and Their Putative Role in Neuronal Regeneration

Alesci,

Fumia,

Mastrantonio

et al. 2024

Fishes

Self Cite

View full text Add to dashboard Cite

Invertebrates show great diversity in their responses to neural damage. Numerous invertebrate phyla, including gastropods, can replace all or a portion of their nervous systems. Aplysia species have been utilized extensively in toxicology, ecology, and neuroscience because their neurological systems react to bodily harm by releasing trophic substances that can stimulate non-neural tissue regeneration and induce changes in the nervous system. This study aims to assess the putative role of hemocytes of Aplysia depilans (Gmelin, 1791) by analyzing the presence of Toll-like receptor 2, inducible nitric oxide synthetase, and, in particular, vimentin and α-tubulin, molecules potentially implicated in the process of neural regeneration. The results demonstrate that all the aforementioned proteins are present in hemocytes, suggesting their role in the defense response and their possible contribution to the neuronal regeneration process of this gastropod. These data provide deeper insight into the internal defense system of this mollusk.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional Adaptations of Hemocytes of Aplysia depilans (Gmelin, 1791) and Their Putative Role in Neuronal Regeneration

Alesci,

Fumia,

Mastrantonio

et al. 2024

Fishes

Self Cite

View full text Add to dashboard Cite

show abstract

“…For millions of years, the mucosal surfaces of all vertebrates have been subject to similar evolutionary pressures. The evolutionary conservation of these cells in the phylogenetic lineages of the animal kingdom (Alesci et al, 2023;Renard et al, 2021). In terrestrial and aquatic mammals, including cetaceans, MALTs are widely distributed in various parts of the body and play an important role in defense against various contaminants (Beineke et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemistry of the nasal cavity‐associated lymphoid tissue in the dolphin (Stenella coeruleoalba, Meyen 1833)

Miller,

Lombardo,

Guerrera

et al. 2024

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

The striped dolphin (Stenella coeruleoalba) is a medium‐sized pelagic dolphin with a single external nasal opening (blowhole) located in the rostral and dorsal regions of the skull. The nasal cavity is divided into three sections: the olfactory, respiratory, and vestibular areas. The surface epithelium lining the regio vestibularis is the first tissue in the nose to be directly affected by environmental antigens. Cetaceans have a significant amount of mucosa‐associated lymphoid tissue (MALT) located throughout their bodies. The lymphoid tissue found in the nasal mucosa is known as nose‐ or nasopharynx‐associated lymphoid tissue (NALT). NALT has not yet been studied in dolphins, but it has been identified and documented in humans and laboratory rodents. This study utilized toll‐like receptor 2 (TLR2), CD4, Langerin/CD207, and inducible nitric oxide synthase to characterize, for the first time, immune cells in the mucosal regio vestibularis of the S. coeruleoalba nasal cavity using confocal microscopy immunofluorescence techniques. The findings revealed scattered immune cells immunoreactive to the tested antibodies, present in both the epithelial tissue lining the nasal cavity vestibulum and the underlying connective tissue. This study enhances our comprehension of the immune system of cetaceans.Research Highlights This study provides new insights into NALT in S. coeruleoalba. This research deepens the knowledge of the skin of cetaceans.

show abstract

“…The incubation of secondary antibodies was carried out after one night of exposition to the primary antibodies. To prevent photobleaching, the slices were mounted using Fluoromount [ 40 , 41 ]. As a negative control, experiments were carried out without the primary antibodies.…”

Section: Methodsmentioning

confidence: 99%

Internal Defense System of Mytilus galloprovincialis (Lamarck, 1819): Ecological Role of Hemocytes as Biomarkers for Thiacloprid and Benzo[a]Pyrene Pollution

Alesci,

Di Paola,

Fumia

et al. 2023

Toxics

Self Cite

View full text Add to dashboard Cite

The introduction of pollutants, such as thiacloprid and benzo[a]pyrene (B[a]P), into the waters of urbanized coastal and estuarine areas through fossil fuel spills, domestic and industrial waste discharges, atmospheric inputs, and continental runoff poses a major threat to the fauna and flora of the aquatic environment and can have a significant impact on the internal defense system of invertebrates such as mussels. Using monoclonal and polyclonal anti-Toll-like receptor 2 (TLR2) and anti-inducible nitric oxide synthetase (iNOS) antibodies for the first time, this work aims to examine hemocytes in the mantle and gills of M. galloprovincialis as biomarkers of thiacloprid and B[a]P pollution and analyze their potential synergistic effect. To pursue this objective, samples were exposed to the pollutants, both individually and simultaneously. Subsequently, oxidative stress biomarkers were evaluated by enzymatic analysis, while tissue changes and the number of hemocytes in the different contaminated groups were assessed via histomorphological and immunohistochemical analyses. Our findings revealed that in comparison to a single exposure, the two pollutants together significantly elevated oxidative stress. Moreover, our data may potentially enhance knowledge on how TLR2 and iNOS work as part of the internal defense system of bivalves. This would help in creating new technologies and strategies, such as biosensors, that are more suitable for managing water pollution, and garnering new details on the condition of the marine ecosystem.

show abstract

Morpho-structural adaptations of the integument in different aquatic organisms

Cited by 5 publications

References 49 publications

Functional Adaptations of Hemocytes of Aplysia depilans (Gmelin, 1791) and Their Putative Role in Neuronal Regeneration

Functional Adaptations of Hemocytes of Aplysia depilans (Gmelin, 1791) and Their Putative Role in Neuronal Regeneration

Immunohistochemistry of the nasal cavity‐associated lymphoid tissue in the dolphin (Stenella coeruleoalba, Meyen 1833)

Internal Defense System of Mytilus galloprovincialis (Lamarck, 1819): Ecological Role of Hemocytes as Biomarkers for Thiacloprid and Benzo[a]Pyrene Pollution

Contact Info

Product

Resources

About