Morphofunctional Differences of Micronuclei in Cultures of Human p53-Positive and p53-Negative Tumor Cells

Sutyagina, Oksana; Kisurina-Evgenieva, O. P.

doi:10.1007/s10517-019-04629-3

Cited by 4 publications

(6 citation statements)

References 10 publications

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“…Micronuclei derived from bridges and nuclear buds are less frequently associated with lamin‐B compared to those derived from laggards 33,37 . Micronuclei of small sizes are more likely to be deficient for lamin‐B than those of large ones 38,39 . Micronuclei in p53‐deficient cells more often have lamin‐B defects than those in p53‐wildtype cells 39 .…”

Section: Not All Micronuclei Are Equal: Heterogeneities In the Sourcementioning

confidence: 98%

“…In this sense, replication errors may be the primary trigger of mutation bursts in micronucleus (Figure 2). While replication defects in micronucleus induce asynchrony in the timing and rate of DNA replication between micronucleus and main nucleus, 39,60 the identical kinetics of NE and mNE breakdown in most micronucleated cells result premature mNE breakdown 24 . Premature mNE breakdown causes the incompletely replicated micronucleus to undergo premature chromosome condensation (PCC) even though it remains in S phase, which may shatter the micronucleus into multiple pieces.…”

Section: Micronuclei Prefer For Rapid Mutagenesismentioning

confidence: 99%

“…When plated as single cells, micronucleated p53‐knockout RPE‐1 cells are more effective in forming clones than micronucleated p53‐wildtype cells (17% vs 1%) 11 . On the other hand, only 40% of micronuclei causes p53 activation and the ensuing cell cycle arrest in p53‐wildtype MCF‐7 cells 39 . Over 40% of the spontaneously micronucleated and over 20% of the chemical‐induced micronucleated cells continue to divide (one cycle to unrestrained cycles) in p53‐wildtype RPE‐1 cells 78,96 .…”

Section: Long‐term Mutational and Functional Landscapes Of Micronuclementioning

confidence: 99%

“…33,37 Micronuclei of small sizes are more likely to be deficient for lamin-B than those of large ones. 38,39 Micronuclei in p53-deficient cells more often have lamin-B defects than those in p53-wildtype cells. 39 mNE also manifests heterogeneity in the presence or absence of lamin-A/C.…”

Section: Introductionmentioning

confidence: 99%

“…38,39 Micronuclei in p53-deficient cells more often have lamin-B defects than those in p53-wildtype cells. 39 mNE also manifests heterogeneity in the presence or absence of lamin-A/C. 40 The birth of mNE-deficient micronuclei may be the cumulative effects of their intracellular geographic origins, biophysical properties and specific mNE features (See Reference 41 for more details).…”

Section: Introductionmentioning

confidence: 99%

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Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

Guo

Dai

et al. 2020

Intl Journal of Cancer

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Micronuclei, small spatially-separated, nucleus-like structures, are a common feature of human cancer cells. There are considerable heterogeneities in the sources, structures and genetic activities of micronuclei. Accumulating evidence suggests that micronuclei and main nuclei represent separate entities with respect to DNA replication, DNA damage sensing and repairing capacity because micronuclei are not monitored by the same checkpoints nor covered by the same nuclear envelope as the main nuclei. Thus, micronuclei are spatially restricted "mutation factories." Several large-scale DNA sequencing and bioinformatics studies over the last few years have revealed that most micronuclei display a mutational signature of chromothripsis immediately after their generation and the underlying molecular mechanisms have been dissected extensively. Clonal expansion of the micronucleated cells is contextdependent and is associated with chromothripsis and several other mutational signatures including extrachromosomal circular DNA, kataegis and chromoanasynthesis. These results suggest what was once thought to be merely a passive indicator of chromosomal instability is now being recognized as a strong mutator phenotype that may drive intratumoral genetic heterogeneity. Herein, we revisit the actionable determinants that contribute to the bursts of mutagenesis in micronuclei and present the growing number of evidence which suggests that micronuclei have distinct shortand long-term mutational and functional effects to cancer genomes. We also pose challenges for studying the long-term effects of micronucleation in the upcoming years.

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Section: Not All Micronuclei Are Equal: Heterogeneities In the Sourcementioning

confidence: 98%

Section: Micronuclei Prefer For Rapid Mutagenesismentioning

confidence: 99%

Section: Long‐term Mutational and Functional Landscapes Of Micronuclementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

Guo

Dai

et al. 2020

Intl Journal of Cancer

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Chromoanagenesis phenomena and their formation mechanisms

et al. 2021

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Possibilities of using micronucleus analysis to detect gene mutations in animals

Novgorodova

2023

Agrarnaâ nauka

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Relevance. The main purpose of micronuclear analysis is to identify substances that cause cytotoxic damage and lead to the formation of micronuclei containing lagging fragments of chromosomes or whole chromosomes. For the study of chromosomal disorders, micronuclear analysis is now very often used, since it is quite easy to perform and does not require large costs.Methods and results. Micronuclei are cytoplasmic chromatin-containing bodies formed when delayed in anaphase or telophase during cell division or fragmentation of the nucleus during apoptosis. The method of micronuclear analysis is used as a biomarker of chromosomal aberrations in the study of mutagenicity, as well as a marker of the development of oncological diseases. With quantitative changes in DNA in the cell, the formation of micronuclei occurs. It is their presence that is an indicator that the body was exposed to environmental pollution (pesticides, pesticides, radiation, heavy metals, etc.), leading to inflammatory and pathological processes. Recently, many studies have been conducted aimed at studying the influence of environmental factors on the genetic apparatus of the body. Under the influence of mutagens, micronuclei are formed in proliferating cells, the counting of which allows for the diagnosis of both genotoxic stress and genetic instability of the organism. To determine early genetic changes in humans and animals, sensitive and labor-intensive methods are needed, which can be attributed to micronuclear analysis. Recently, micronuclear analysis has become relevant for its use in the livestock sector, mainly in research aimed at quality control of livestock products used in the future for human nutrition.

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Morphofunctional Differences of Micronuclei in Cultures of Human p53-Positive and p53-Negative Tumor Cells

Cited by 4 publications

References 10 publications

Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

Chromoanagenesis phenomena and their formation mechanisms

Possibilities of using micronucleus analysis to detect gene mutations in animals

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