Morphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes

Suzuki, Ryosuke; Okada, Mai; Nagai, Hiroki; Kobayashi, Jun’ichi; Sugimura, Satoshi

doi:10.1016/j.theriogenology.2021.02.021

Cited by 12 publications

(10 citation statements)

References 50 publications

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“…For normal bovine zygotes (with two pronuclei) that resulted in blastocysts, the time-points from IVF to pronuclei appearance and fading were 10.4 h and 25.5 h, respectively (Suzuki et al, 2021). In the present study, 8 of 23 zygotes formed male and female pronuclei 18 h after ICSI, within the expected period of pronuclear appearance to fading (10.4~25.5h).…”

Section: Discussionsupporting

confidence: 51%

Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes

Liu¹,

Ma²,

Liu

et al. 2022

Anim. Reprod.

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The objective of this study was to evaluate the fertilization capability of White Bengal Tiger frozen-thawed completely immotile spermatozoa after interspecific intracytoplasmic sperm injection (ICSI) with bovine oocytes. The fertilization status of presumptive zygotes was assessed 18 h after ICSI by immunofluorescence staining and confocal microscopy. The fertilization rate was 34.8% (8/23), as confirmed by the extrusion of two polar bodies, or male and female pronuclei formation. For unfertilized oocytes (65.2%, 15/23), one activated oocyte had an activated spermatozoon but most were unactivated oocytes with unactivated spermatozoa (1/15, 6.7% vs 10/15, 66.7%, respectively, p < 0.05). These results showed that White Bengal Tiger frozen-thawed completely immotile spermatozoa retained the capacity to fertilize bovine oocytes after interspecific ICSI. This is the first report of in vitro produced zygotes using tiger immotile sperm with bovine oocytes by interspecific ICSI technique, which provides an efficient and feasible method for preservation and utilization of endangered feline animals.

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Section: Discussionsupporting

confidence: 51%

Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes

Liu¹,

Ma²,

Liu

et al. 2022

Anim. Reprod.

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“…Considering that bovine and non-human primate embryogenesis parallel human embryogenesis, including the chromosomal instability [12,[66][67][68][69][70], we hypothesized mixoploidy and/or chimerism to be traceable in human cleavage-and blastocyst-stage embryos. In two human embryos, a gynogenetic blastomere at day-3 biopsy was identified through comprehensive haplotyping-based preimplantation genetic testing (PGT).…”

Section: Genome-wide Mosaicism Exists In Human Blastocystsmentioning

confidence: 99%

Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts

Coster

Masset²,

Tšuiko³

et al. 2022

Genome Biol

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Background During normal zygotic division, two haploid parental genomes replicate, unite and segregate into two biparental diploid blastomeres. Results Contrary to this fundamental biological tenet, we demonstrate here that parental genomes can segregate to distinct blastomeres during the zygotic division resulting in haploid or uniparental diploid and polyploid cells, a phenomenon coined heterogoneic division. By mapping the genomic landscape of 82 blastomeres from 25 bovine zygotes, we show that multipolar zygotic division is a tell-tale of whole-genome segregation errors. Based on the haplotypes and live-imaging of zygotic divisions, we demonstrate that various combinations of androgenetic, gynogenetic, diploid, and polyploid blastomeres arise via distinct parental genome segregation errors including the formation of additional paternal, private parental, or tripolar spindles, or by extrusion of paternal genomes. Hence, we provide evidence that private parental spindles, if failing to congress before anaphase, can lead to whole-genome segregation errors. In addition, anuclear blastomeres are common, indicating that cytokinesis can be uncoupled from karyokinesis. Dissociation of blastocyst-stage embryos further demonstrates that whole-genome segregation errors might lead to mixoploid or chimeric development in both human and cow. Yet, following multipolar zygotic division, fewer embryos reach the blastocyst stage and diploidization occurs frequently indicating that alternatively, blastomeres with genome-wide errors resulting from whole-genome segregation errors can be selected against or contribute to embryonic arrest. Conclusions Heterogoneic zygotic division provides an overarching paradigm for the development of mixoploid and chimeric individuals and moles and can be an important cause of embryonic and fetal arrest following natural conception or IVF.

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“…These studies provide an essential foundation for the progress of TLM in bovine IVF embryos. Subsequently, several studies have validated the association of morphokinetic and morphological parameters of bovine IVF embryos with developmental ability and implantation potential after transfer [ 11 , 53 , 54 , 55 , 56 , 57 , 58 ]. However, this information is limited compared to that of humans, and commercial and practical applications have not yet been achieved.…”

Section: Progress Of Tlm Technology In Bovine Embryo Productionmentioning

confidence: 99%

Time-lapse monitoring technologies for the selection of bovine <i>in vitro</i> fertilized embryos with high implantation potential

Magata

2023

J. Reprod. Dev.

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Over the years, the utilization of in vitro fertilization (IVF) in bovine embryo production has increased globally to accelerate the selection of cows with high genetic values. The selection of embryos with high implantation potential is a critical factor in establishing pregnancy. Time-lapse monitoring (TLM) has emerged as a new technique that allows frequent and non-invasive imaging of developing embryos.TLM is considered to have several advantages over the conventional morphological evaluation of embryos, which has been widely used in bovine embryo production. Establishing a novel embryo selection algorithm specifically for bovine IVF embryos is a critical challenge, but information on the association between morphokinetic data obtained using TLM and the implantation potential of embryos is still limited. This review outlines the potential application of TLM technology to improve the fertility of bovine IVF embryos, focusing on the results of human and bovine TLM studies that can be applied to select bovine embryos with high implantation potential. First, the progress of the TLM technology in bovine embryo production is summarized. The association between kinetic and morphological parameters and the developmental and implantation potential of human and bovine embryos is outlined.Finally, the benefits of evaluating blastocyst collapse and re-expansion as indicators of bovine embryo viability and the possible application of TLM to detect chromosomal abnormalities and determine embryo sex will be discussed.

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Morphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes

Cited by 12 publications

References 50 publications

Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes

Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes

Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts

Time-lapse monitoring technologies for the selection of bovine <i>in vitro</i> fertilized embryos with high implantation potential

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