Morphologic and genetic analysis for geographic populations of greenbug Schizaphis graminum (Hemiptera: Aphididae) in Egypt

Tabikha, Reda M.; Adss, Ibrahim A.

doi:10.2478/s11756-020-00501-2

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…Reaction of 6 arbitrary primers and 4 ISSRs primers with genomic DNA of 16 geographical specimens for R. padi revealed that those primers produced 416 polymorphic DNA fragments to achieve polymorphism 47.27% among the examined populations (Tabikha and Adss 2016).Although some banding patterns of UBC826 and UBC827 primers were polymorphic with the tested aphid species, they were monomorphic for most geographic populations of S. graminum in Egyptian agro-ecosystems (Tabikha and Adss 2021), which indicated that those primers are more convenient to determine different aphid species than geographical populations of specific species. While in current study, S. graminum could be determined by absence of the 610 bp band that was generated by ISSRs primer of UBC818.…”

Section: Banding Patterns Analysismentioning

confidence: 99%

“…Thus, Amin et al (2013) subjected 18 unique DNA marker to construct molecular branching key that differentiate 13 aphid species. Moreover, Tabikha and Adss (2021) proposed a molecular branching key that based on six ISSRs primers (UBC-840, UBC-808, UBC-814, UBC-868, HB-12 and UBC-811) and 16 polymorphic DNA fragments with different molecular sizes, to discriminate 16 geographic populations of S. graminum that habitat Egyptian wheat fields.…”

Section: Molecular Taxonomic Keysmentioning

confidence: 99%

“…Moreover, Gui et al (2008) reported that ISSR is considered as specific technique to detect genetic variability among insects' species. Thus, it could successfully identify two aphid species "Pemphigus obesinymphae and Acyrthosiphon pisum" (Abbot 2001), characterize cereal aphid species (Helmi and Khafaga 2011), and differentiate geographical populations for each of Rhopalosiphum padi (Tabikha and Adss 2016) and S. graminum (Tabikha and Adss 2021) in Egypt. In addition, biotypes of insects such as Schizaphis graminum could be also differentiated by ISSRs (Weng et al 2007).…”

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confidence: 99%

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Identification and Reconsidering Phylogeny of Some Aphid Species, (Hemiptera: Aphididae), Based on Molecular DNA Markers Using ISSRs-PCR Technique

Adss

Tabikha

2021

Arab Universities Journal of Agricultural Sciences

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Aphids are considered as most economically importance and worldwide insect pests. Successful pest management systems are based on accurate and rapid pests' species identification. Traditional morphological identification of closed aphid species may be considered as inaccurate taxonomic process. For overcoming disadvantages of traditional morphological identification, molecular techniques, related to DNA markers and based on polymerase chain reaction (PCR), were approached by using nine ISSRs primers to identify and diagnose fifteen common aphid species that disperse in Egyptian agro-ecosystem. The examined ISSRs primers could successfully discriminate the tested aphid species that reflected 61.39% polymorphism among them. Moreover, four banding patterns were considered as unique bands which could characterize three aphid species (Aphis gossypii, Aphis nerii and Myzus persicae). Highest genetic homology (84.9%) was observed between species Rhopalosipum padi and Schizaphis graminum. In additions, each of A. gossypii and Aphis citricola were also genetically homologous species. In contrast, species Aphis craccivora and M. persicae were analogous genetically with low similarity percentile (59.8%). High genetic divergence was observed also between A. nerii and M. persicae. Two alternative molecular branching taxonomic keys were proposed by subjecting five highest polymorphic ISSRs primers and 29 banding patterns with different molecular sizes.

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Section: Banding Patterns Analysismentioning

confidence: 99%

Section: Molecular Taxonomic Keysmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification and Reconsidering Phylogeny of Some Aphid Species, (Hemiptera: Aphididae), Based on Molecular DNA Markers Using ISSRs-PCR Technique

Adss

Tabikha

2021

Arab Universities Journal of Agricultural Sciences

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“…Due to the economic importance of aphids, several studies have been conducted in Egypt (e.g. Attia et al (1986), Darwish (1983, El-Heneidy and Adly (2012), Tabikha and Adss (2021).…”

Section: Introductionmentioning

confidence: 99%

Greenidae ficicola (Takahashi) (Hemiptera: Sternorrhyncha: Aphididae) newly recorded in Egypt

Ramadan

Tawfeek

2022

Alexandria Journal of Agricultural Sciences

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Greenidea ficicola aphid was recorded in this work for the first time in Ficus trees in April 2022 in Alexandria, Egypt. This species was mainly found to infest ornamental Ficus trees which are widely used in landscapes. G. ficicola is pear-shaped, yellowish-brown to dark brown. Nymphs are yellow with brown siphuniculi. G. ficicola is characterized by a weekly-developed antennal tubercle, broadly rounded cauda with apical papilla, and conspicuous setae arising from dark patches. Alate with elongated body dark abdomen supported with longer siphunculi. Despite the distribution of this species in Asia, Nearctic, Neotropic, West Palaearctic, Afrotropical and Australian origins, it is recorded here for the first time from Egypt. Apart from the wilt caused through the plant juice suction via the infestations of this pest, several aphidborne viruses are being transmitted to the Ficus plants, leading to tremendous economic loss.

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Genetic diversity analysis of Saccharosydne procerus in Hunan region, China

Shan,

Wu,

Ding

et al. 2024

Mol Biol Rep

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Morphologic and genetic analysis for geographic populations of greenbug Schizaphis graminum (Hemiptera: Aphididae) in Egypt

Cited by 4 publications

References 40 publications

Identification and Reconsidering Phylogeny of Some Aphid Species, (Hemiptera: Aphididae), Based on Molecular DNA Markers Using ISSRs-PCR Technique

Identification and Reconsidering Phylogeny of Some Aphid Species, (Hemiptera: Aphididae), Based on Molecular DNA Markers Using ISSRs-PCR Technique

Greenidae ficicola (Takahashi) (Hemiptera: Sternorrhyncha: Aphididae) newly recorded in Egypt

Genetic diversity analysis of Saccharosydne procerus in Hunan region, China

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