Morphologic and molecular identification of three macroscopic Sarcocystis species infecting domestic sheep (Ovis aries) and cattle (Bos taurus) in Egypt

El-Morsey, Ahmed; Abdo, Walied; Zaid, Attia A. Abou; Sorour, Shimaa Sobhy

doi:10.1007/s00436-020-07002-w

Cited by 10 publications

(15 citation statements)

References 42 publications

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“…in sheep was reported in Iran, reaching up to 37% [ 16 , 17 , 32 , 36 , 37 ]. Sarcocystis gigantea is assumed to be a more frequent macroscopic species as compared to S. medusiformis [ 17 , 18 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Identification of Sarcocystis Species in Sheep from Lithuania

Marandykina-Prakienė

Butkauskas

Gudiškis

et al. 2022

Animals

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Data on the distribution of different Sarcocystis species in various muscles of sheep are scarce. In the present study, 190 diaphragm, oesophagus, and heart muscle samples of 69 sheep raised in Lithuania were examined for the presence of Sarcocystis spp. Under a light microscope, two morphological types of microcysts corresponding to S. arieticanis and S. tenella were detected. Eight and 12 sarcocysts of S. arieticanis and S. tenella, respectively, were isolated and characterised by the sequencing of a portion of cox1. The sequence comparisons revealed the highest similarity between European and Asian isolates of S. arieticanis and S. tenella obtained from domestic sheep and other wild Caprinae hosts. Based on peptic digestion, nested PCR targeting cox1, and sequencing, a 100% infection prevalence of S. arieticanis and S. tenella was observed in the 69 studied animals. The occurrence of S. tenella was significantly higher in the diaphragm than in the oesophagus (χ2 = 13.14, p < 0.001), whereas differences in the prevalence of S. arieticanis in the studied muscle types were insignificant (χ2 = 1.28, p > 0.05). Further molecularly based epidemiological studies are needed to compare the prevalence of Sarcocystis species in various muscles of sheep raised in different geographic regions.

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Section: Discussionmentioning

confidence: 99%

“…sporocysts, which leads to infection. On the other hand, the limiting factor of exposure to feline faeces is that cats tend to bury their excrement [ 38 , 39 ]. Furthermore, macroscopic cysts of S. gigantea and S. medusiformis tend to require longer time periods (from 1 to 2 years) to grow and fully develop [ 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Sarcocystis Species in Sheep from Lithuania

Marandykina-Prakienė

Butkauskas

Gudiškis

et al. 2022

Animals

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“…The isolated macrosarcocysts were processed for TEM as previously described by (El-Morsey 2010 , 2016 ; El-Morsey et al 2014 , 2015a , 2019 , 2021 ). Briefly, portions of the sarcocysts fixed in 2.5% glutaraldehyde were post-fixed in osmium tetroxide 1%, dehydrated in serially graded alcohols, and finally embedded in an epon-araldite mixture.…”

Section: Methodsmentioning

confidence: 99%

“…Macroscopic lesions associated with eosinophilic myositis as a consequence of bovine or ovine Sarcocystis spp. encystation in cattle, sheep, and goat musculature, or even merogony stages associated with granulomatous reactions might appear grossly as yellowish-white spots or cyst-like lesions, in some cases on the external surfaces of the internal organs such as the liver, kidney, lung, or spleen of the animal during carcass inspection in abattoirs (El-Morsey 2010 ; El-Seify et al 2014 ; Dubey et al 2016 ; Gjerde et al 2020 ; El-Morsey et al 2021 ). Additionally, the adverse pathogenic effects on the intermediate host as, abortion, fever, anemia, anorexia, and even deaths, more specifically due to infection by some of the canine-transmitted Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

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Recent insights into the morphology, molecular characterization and tissue localization of the caprine Sarcocystis species infecting domestic goats (Capra hiricus): Sarcocystis moulei, Sarcocystis capracanis, and Sarcocystis hircicanis

El-Morsey,

Abdo

2023

Parasitol Res

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Ninety-seven (64.67%) out of 150 domestic goats (Capra hiricus) carcasses were found to be infected by Sarcocystis moulei, Sarcocystis capracanis, and Sarcocystis hircicanis sarcocysts. Sarcocystis moulei macrosarcocysts were detected in the cardiac, esophageal, skeletal, lingual, and diaphragmatic muscles of seven goats (4.67%) out of the 150 examined animals, whereas the microscopic Sarcocystis species were found in (90/150 = 60%). Two morphotypes of S. moulei were observed. Morphotype (I) macrosarcocysts were large-sized oval, ovoid, spherical, and measured 2–7 mm in length x 2–6 mm in width. Sarcocystis moulei morphotype (II) macrosarcocysts were spindle-shaped, spheroid, sometimes elongated, and measured 1.8–6 x 0.5–2 mm. By TEM, all S. moulei morphotypes were ultrastructurally the same and had a sarcocyst wall that was characterized by highly branched or cauliflower-like villar protrusions (VP) with dumbbell-like structures. The VP interior was packed with well-developed microtubules in longitudinal and cross arrangements. Sarcocystis moulei cyst wall was 3–6 μm thick. Sarcocystis capracanis microsarcocysts detected herein had a cyst wall that ranged from 4–8 μm in thickness. The VP was upright finger-like or cylindrical. The PVM had electron-dense corrugations in the region of the VP. Few amounts of microfilaments were detected inside the cores of VP. Sarcocystis hircicanis had a thinner cyst wall (~1–3 μm) with hairy long VP that ranged from 1 to 7.5 μm in length. Microtubules were missing inside the cores of the VP. The three caprine Sarcocystis species were molecularly characterized on the level of the 18S rRNA, 28S rRNA, and Cox1 genes.

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A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

et al. 2022

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Infections with the Apicomplexa Toxoplasma gondii, Neospora caninum, and Sarcocystis spp. are common causes of reproductive disorders in sheep. However, few epidemiological studies regarding co-infections with these three protozoa are reported in sheep in Italy. For this reason, this study aims to evaluate possible co-infections with T. gondii, N. caninum, and Sarcocystis spp. in sheep slaughtered for human consumption. From April to July 2019, individual blood, brain, heart, and diaphragm samples were collected from 138 sheep after slaughtering. The presence of IgG anti-Toxoplasma in serum samples was evaluated through ELISA. DNA of the three protozoa was investigated using specific PCRs. Co-infection with T. gondii, N. caninum and Sarcocystis spp. was found in 66.7% of the examined sheep. Antibodies against T. gondii were found in the 36.2% of serum samples. The presence of T. gondii DNA was detected in the 67.4%, 77.5%, and 21.7% of the brain, heart, and diaphragm samples, respectively. Neospora caninum DNA was found in 72.5% of the examined brain samples. Sarcocystis spp. DNA was detected in 92% and 52.2% of the heart and diaphragm samples, respectively. Sequence analysis of the Sarcocystis spp. revealed the sole presence of Sarcocystis tenella. The present study demonstrates that sheep have a high risk of infection with the three Apicomplexa investigated, suggesting the need to adopt adequate measures to prevent the spread of these parasitic infections considering their clinical and economic impact on ovine production. Furthermore, the possible role sheep play in the zoonotic transmission of toxoplasmosis to humans was highlighted.

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Morphologic and molecular identification of three macroscopic Sarcocystis species infecting domestic sheep (Ovis aries) and cattle (Bos taurus) in Egypt

Cited by 10 publications

References 42 publications

Molecular Identification of Sarcocystis Species in Sheep from Lithuania

Molecular Identification of Sarcocystis Species in Sheep from Lithuania

Recent insights into the morphology, molecular characterization and tissue localization of the caprine Sarcocystis species infecting domestic goats (Capra hiricus): Sarcocystis moulei, Sarcocystis capracanis, and Sarcocystis hircicanis

A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

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