Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil

Ferreira, Adelina; Mehanna, Mahmoud; Prado, Cynthia P. A.

doi:10.32604/biocell.2008.32.185

Cited by 20 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although in general the histomorphological organization of Leptodactylus chaquensis testis and the gametogenic process are similar to those described for other anuran amphibia such as Caudiverbera caudiverbera (Hermosilla et al ., 1983), Hyla japonica (Lee & Kwon, 1992 ) , Scinax fuscovarius (Oliveira & Vicentini, 1998), Physalaemus cuvieri (Oliveira et al ., 2002), Pseudis limellum (Ferreira et al ., 2008) and Dendropsophus minutus (Santos & Oliveira, 2008), they also present characteristics of their own.…”

Section: Discussionmentioning

confidence: 99%

“…All cells of the germ line are present in the two periods studied. This fact allows us to suggest that Leptodactylus chaquensis could be an amphibian with a potentially continuous reproductive cycle similar to that of other species such as Telmatobius laticeps and Telmatobius pisanoi (Montero & Pisanó, 1990), Hyla pulchella andina (Montero & Pisanó, 1992), Pseudis limellum (Ferreira et al ., 2008), Dendropsophus minutus (Santos & Oliveira, 2008). In fact, in Leptodactylus chaquensis it has been found that spermiation can be obtained under hormonal stimulation at any time of the year, a proposal that agrees with the results reported for Bufo arenarum (Canosa et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast with the results reported for Rana esculenta (Rastogi et al ., 1985) and Pachymedusa dacnicolor (Rastogi et al ., 1988), in which dark and clear primary spermatogonia were described, in Leptodactylus chaquensis only one class could be identified. Our observations are in agreement with those described for Bufo arenarum (Cavicchia & Moviglia, 1983), Hyla ranki (Taboga & Dolder, 1991), Scinax fuscovarius (Oliveira & Vicentini, 1998), Physalaemus cuvieri (Oliveira et al ., 2002), Dendropsophus minutus (Santos & Oliveira, 2008) and Pseudis limellum (Ferreira et al ., 2008).…”

Section: Discussionmentioning

confidence: 99%

“…This intercellular communication between germ cells would allow molecular exchange (Gilbert, 2000) that could be involved in the synchronization of the spermatogenic process. Our observations revealed the presence of intercellular spaces, which gradually appeared from spermatocyte stages and became more evident in those cysts constituted by spermatids as described for other amphibian species (Hermosilla et al ., 1983; Ferreira et al ., 2008). Even if the intercellular spaces increased in size as spermatogenesis progressed, in Leptodactylus chaquensis they did not become joined to form a central vacuole as reported by Lofts (1974) for Rana temporaria .…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Spermatogenesis inLeptodactylus chaquensis. Histological study

Villagra

Cisint

Crespo

et al. 2012

Zygote

View full text Add to dashboard Cite

The organization and the histological characteristics of Leptodactylus chaquensis testis throughout the reproductive cycle were analyzed in the presented study. Gonads of adult males, processed with routine techniques for optical microscopy, revealed that during the reproductive period the seminiferous tubules were characterized by presentation of a large number of cysts, germ cells at the same maturation stage supported by Sertoli cells. All the germ line cells were also present in the postreproductive period and maintained their morphological characteristics. Primary spermatogonia were large-sized cells found isolated or in small groups. The rest of the cells of the germ line formed cysts. Secondary spermatogonia showed morphological characteristics similar to their predecessors, although they were smaller. Primary and secondary spermatocytes showed images of the different stages of the first and second meiotic division respectively. One finding was the presence of intercytoplasmic bridges between the secondary spermatocytes. Primary spermatids were rounded cells with an acrosomal vesicle associated with the nucleus and had cysts that were characterized by large intercellular spaces. Secondary spermatids were elongated cells with a well defined acrosome, which in the spermatozoa had the shape of an arrowhead. Another peculiar characteristic of this species was the fusion of the walls of the seminiferous tubule with the efferent duct that formed a path for spermatozoa during spermiation. The presence in the seminiferous tubules of all stages of the spermatogenic line during the two periods of the cycle studied indicated that Leptodactylus chaquensis had a potentially continuous reproductive cycle.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Spermatogenesis inLeptodactylus chaquensis. Histological study

Villagra

Cisint

Crespo

et al. 2012

Zygote

View full text Add to dashboard Cite

show abstract

“…Κνηλφ ραξαθηεξηζηηθφ ηνπ κεγαιχηεξνπ αξηζκνχ ησλ εξεπλψλ πνπ γίλνληαη βαζηδφκελεο ζε απηφ ην πξφγξακκα είλαη ε εθαξκνγή ησλ πξνο κειέηε αληηθεηκέλσλ ζε κνξθνκεηξηθά πιαίζηα. (168)(169)(170)(171)(172)(173)(174)(175)(176)(177)(178)(179)(180)(181) Σηο εθαξκνγέο απηέο εθκεηαιιεπηήθακε ζηελ πνξεία ηεο δηεμαγσγήο ηεο παξνχζαο έξεπλαο. Σα βήκαηα πνπ αθνινπζήζεθαλ γηα ηε κέηξεζε ηνπ ειεχζεξνπ απινχ παξνπζηάδνληαη ζπλνπηηθα ζηηο παξαθάησ εηθφλεο .…”

Section: υαρακσζρησηκα σοτ προγραμμασοunclassified

Μορφομετρική Ανάλυση Της Σύνθεσης Της Αθηρωματικής Πλάκας Στις Στεφανιαίες Αρτηρίες Στον Αιφνίδιο Στεφανιαίο Θάνατο

Papavdi¹,

Παπαβδή²

View full text Add to dashboard Cite

Diseases of the arteries are responsible for morbidity and mortality more than any other human disease. The anomalies and pathologies of arteries cause clinical disease in two basic mechanisms, narrowing or complete obstruction of the lumen or the presence of acute phenomenon (eg, thrombosis, embolism). Theories about the inaugural events of atherogenesis started out early, but today dominates the theory of the inflammatory response to endothelial damage due to synergistic hemodynamic disorders, adverse effects of hypercholesterolemia and contribution of infectious agents. The most studied situation which contributes greatly both to the genesis and the evolution of the atherosclerotic lesion is hypercholesterolemia, which through production of free radicals, causes endothelial damage, dysfunction, and the creation of increased levels of oxidized subendothelial LDL.Exploring the epidemiological characteristics, regarding the symptoms and therefore sudden death, these are observed at much younger ages, which often involve the 3 rd decade of life, while in the genesis and progression are involved both familial and environmental factors. Each plaque has a starting point of genesis, where the vascular environment and the above factors regulate its further development. As the earlier phase is defined the fatty streak, while the lumen of the vessel may be blocked gradually or after an acute episode. In any case, the lethal mechanism is the release of ventricular arrhythmias or fibrillation, after coronary spasm. The concept of vulnerable plaque has been introduced in recent years, explaining how plaques causing mild stenosis in the angiographic findings, give birth to acute coronary events and are prone to thrombosis, which is usually come after a rupture or erosion of plaque.180 patients died of sudden coronary death constituted the study material as well as 40 witnesses, with extracardiac cause of death. The average stenosis was calculated digitally at 86.1%, while the microscopic examination showed 87.3%. Specifically, they found 101 cases out of 180 total observations in which the digitally measured stenosis was less than originally estimated, and 21 cases in which the final measured lumen was narrowed by less than 75%. The difference in measurements is attributed to the wrong microscopic estimation in the first place, the error which is included in the measurement after processing tissues with formalin and parafin, not taking into account the shape of the plaque and the extent of the damage in the coronary arterial tree, regardless of the degree of stenosis caused by them.The main conclusion resulting from the second part of the study is that the plaque does not grow by getting through specific and predetermined stages, increasing proportionally the composition of its various components, but reasonably present differences between them and each one follows a different pattern , while the opposite results would indicate a linear and smooth volume change of the plaque with the ingredients.

show abstract

A test for plasticity in sperm motility activation in response to osmotic environment in an anuran amphibian

Byrne

Anastas

Silla

2022

Ecology and Evolution

View full text Add to dashboard Cite

Evolutionary theory predicts that selection will favor phenotypic plasticity in sperm traits that maximize fertilization success in dynamic fertilization environments. In species with external fertilization, osmolality of the fertilization medium is known to play a critical role in activating sperm motility, but evidence for osmotic‐induced sperm plasticity is limited to euryhaline fish and marine invertebrates. Whether this capacity extends to freshwater taxa remains unknown. Here, we provide the first test for plasticity in sperm‐motility activation in response to osmotic environment in an anuran amphibian. Male common eastern froglets ( Crinia signifera ) were acclimated to either low (0 mOsmol kg −1 ) or high (50 mOsmol kg −1 ) environmental osmolality, and using a split‐sample experimental design, sperm were activated across a range of osmolality treatments (0, 25, 50, 75, 100, and 200 ± 2 mOsmol kg −1 ). Unexpectedly, there was no detectable shift in the optimal osmolality for sperm‐motility activation after approximately 13 weeks of acclimation (a period reflecting the duration of the winter breeding season). However, in both the low and high acclimation treatments, the optimal osmolality for sperm‐motility activation mirrored the osmolality at the natural breeding site, indicating a phenotypic match to the local environment. Previously it has been shown that C. signifera display among‐population covariation between environmental osmolality and sperm performance. Coupled with this finding, the results of the present study suggest that inter‐population differences reflect genetic divergence and local adaptation. We discuss the need for experimental tests of osmotic‐induced sperm plasticity in more freshwater taxa to better understand the environmental and evolutionary contexts favoring adaptive plasticity in sperm‐motility activation.

show abstract

Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil

Cited by 20 publications

References 35 publications

Spermatogenesis inLeptodactylus chaquensis. Histological study

Spermatogenesis inLeptodactylus chaquensis. Histological study

Μορφομετρική Ανάλυση Της Σύνθεσης Της Αθηρωματικής Πλάκας Στις Στεφανιαίες Αρτηρίες Στον Αιφνίδιο Στεφανιαίο Θάνατο

A test for plasticity in sperm motility activation in response to osmotic environment in an anuran amphibian

Contact Info

Product

Resources

About