Morphological abnormalities in zebrafish cryopreserved sperm

Costa, Bruna Bitencourt da; Oliveira, Diogo Losh de; Rodrigues, Rômulo Batista; Gomes, Itamar Cossina; Streit, Danilo Pedro

doi:10.1016/j.cryobiol.2020.08.003

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…In contrast, after 6 h of exposure, zebrafish sperm showed significant decreases of the VSL and VCL in all TBPH exposed groups; VAP in all treatments except for 0.1 μM; the progressive motility in 1 μM, 10 μM, and 60 μM TBPH treatments; and the total motility in 10 μM TBPH treatments. These results suggested that TBPH mainly affected the general motility of zebrafish sperm after 3 h exposure but altered the motive tracks of sperm after 6 h exposure, indicating different mechanisms or aggravated toxicity with the extension of time. , Taken together, our results confirmed the toxic effects on zebrafish sperm quality upon ex vivo exposure to TBPH.…”

Section: Results and Discussionsupporting

confidence: 76%

“…These results suggested that TBPH mainly affected the general motility of zebrafish sperm after 3 h exposure but altered the motive tracks of sperm after 6 h exposure, indicating different mechanisms or aggravated toxicity with the extension of time. 34,49 Taken together, our results confirmed the toxic effects on zebrafish sperm quality upon ex vivo exposure to TBPH.…”

Section: In Vitro and Ex Vivo Evidence For Disruptions On Male Reprod...supporting

confidence: 80%

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Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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Bis(2-ethylhexyl)tetrabromophthalate (TBPH) has been widely detected in the environment and organisms; thus, its toxic effects on male reproduction were systematically studied. First, we found that TBPH can stably bind to the androgen receptor (AR) based on in silico molecular docking results and observed an antagonistic activity, but not agonistic activity, on the AR signaling pathway using a constructed AR-GRIP1 yeast assay. Subsequently, we validated the adverse effects on male germ cells by observing inhibited androgen production and proliferation in Leydig cells upon in vitro exposure and affected general motility and motive tracks of zebrafish sperm upon ex vivo exposure. Finally, the in vivo reproductive toxicity was demonstrated in male zebrafish by reduced mating behavior in F0 generation when paired with unexposed females and abnormal development of their offspring. In addition, reduced sperm motility and impaired germ cells in male zebrafish were also observed, which may be related to the disturbed homeostasis of sex hormones. Notably, the specifically suppressed AR in the brain provides further evidence for the antagonistic effects as above-mentioned. These results confirmed that TBPH affected male reproduction through a classical nuclear receptor-mediated pathway, which would be helpful for assessing the ecological and health risks of TBPH.

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Section: Results and Discussionsupporting

confidence: 76%

Section: In Vitro and Ex Vivo Evidence For Disruptions On Male Reprod...supporting

confidence: 80%

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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“…Motility is the most frequently used parameter to assess post thaw sperm quality. It is usually well interconnected with fertility (Costa et al, 2020). The present study found that post thawed sperm cryopreserved with 8% Me 2 SO and 2% MeOH were the most motile and immotile ones, respectively.…”

Section: Discussionsupporting

confidence: 47%

“…Abnormalities including total structure damage, abnormal body shape, coiled tail, and droplet in tail were only observed for cryopreserved sperm. Morphological abnormalities were previously reported from cryopreserved sperm of zebrafish and seven-band grouper (Costa et al, 2020;Kim et al, 2020b). Cryo-injury of post thaw sperm can occur due to several causative factors such as cold shock, ice crystallization, CPA toxicity, pH fluctuation, and osmotic effect (Chao and Liao, 2001;Chatiza et al, 2011;FIGURE 9 | Fertilization and hatching rates of cryopreserved sperm of Pacific abalone (n = 3).…”

Section: Discussionmentioning

confidence: 92%

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage than sperm cryopreserved with other cryoprotectants. Sperm to egg ratio of 10,000:1 was found to be the most suitable ratio for in vitro fertilization among different ratios tested. The fertilization rate of sperm cryopreserved with 8% Me2SO was not significantly (p > 0.05) different from that of sperm cryopreserved with 2% GLY. DNA fragmentation showed strongly negative relationships with sperm quality parameters. Sperm cryopreserved with 8% Me2SO showed higher post thaw quality and mRNA expression of sperm motility associated gene than those cryopreserved with other cryoprotectants. The present research suggests to use 8% Me2SO for cryopreservation of Pacific abalone sperm as well as for hatchery production.

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The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

et al. 2022

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Morphological abnormalities in zebrafish cryopreserved sperm

Cited by 7 publications

References 11 publications

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

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