Morphological and Biochemical Properties Useful for Identification of Erwinia chrysanthemi

Vasundhara, B.A.; Thammaiah, N.

doi:10.20546/ijcmas.2017.610.042

Cited by 2 publications

(7 citation statements)

References 5 publications

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“…Colonies of D. chrysanthemi, D. paradisiaca and P. carotovorum subsp. carotovorum were isolated from different crops, including bananas using NA culture medium, with similar results to those obtained in the present work (Nagrale et al, 2013;Akbar et al, 2015;Czajkowski et al, 2015;Vasundhara and Thammaiah, 2017).…”

Section: A B Csupporting

confidence: 90%

“…carotovorum. Similar results were found by Vasundhara and Thammaiah (2017), who reported that symptoms of the disease by D. chrysanthemi appeared 25 to 30 after inoculation in banana seedlings. Furthermore, Gokul et al (2019) and Ragavi et al (2019) reported that symptoms of complete rot and plant drop caused by P. carotovorum subsp.…”

Section: Isolate Pathogenicity Testsupporting

confidence: 89%

“…Primo Star, Germany). These bacterial pathogens are characterized by presenting perimeter flagella (Agrios, 2008;Aremu and Babalola, 2015;Czajkowski et al, 2015;Blomme et al, 2017;Vasundhara and Thammaiah, 2017), which was observed in all the isolates in the present work.…”

Section: A B Csupporting

confidence: 52%

“…These results agree with those of Nagrale et al (2013) for E. chrysanthemi pv. paradisiaca, Ashmawy et al (2015), Bhupendra and Yogendra (2015), and Vasundhara and Thammaiah (2017), who observed that D. chrysanthemi caused production of indole acid when performing this biochemical test to characterize bacterial isolates.…”

Section: A B Cmentioning

confidence: 99%

“…carotovorum reached maximum growth at 27°C. In addition, Nagrale et al (2013) and Vasundhara and Thammaiah (2017) reported that E. chrysanthemi pv. and physio-biochemical tests can be used for the identification of D. chrysanthemi and P. carotovorum subsp.…”

Section: A B Cmentioning

confidence: 99%

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CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

Rafael-Rutte¹,

Zavala²,

Maldonado³

et al. 2022

Chil. j. agric. anim. sci.

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Organic banana (Musa sp.) is one of the most important export crops in Peru, especially in the Department of Piura. The cultivation of the crop is mainly affected by biotic factors, resulting in important economic losses. The aim of this research was to characterize the causal agent of banana bacteriosis disease in Piura. Bacterial isolates were extracted from rhizome and pseudostem samples of organic bananas with typical symptoms of soft rot from different banana growing areas of Piura. Sixty colonies were obtained, while 10 isolates were selected for presenting cultural and morphological characteristics similar to those of Pectobacterium (01MB, 04MB, 05MB, 06MB and 10MB) and Dickeya (02MB, 03MB, 08MB, 07MB and 09MB); different biochemical and molecular tests were conducted. The pathogenicity of the isolates was confirmed by in vitro and in vivo inoculation of rhizome pieces and pseudostems from cultivated plants. The 10 isolates were gram negative and with perimeter flagella. The biochemical tests showed that all the isolates were positive on nutrient agar medium, Mac Conkey, catalase, gelatin hydrolysis and growth at 37°C, whereas they were negative on King's B medium, nitrate reductase and oxidase tests. Isolates 02MB, 03MB, 08MB, 07MB and 09MB also tested positive for erythromycin and indole, while isolates 01MB, 04MB, 05MB, 06MB and 10MB were positive for CVP (Crystal violet pectate). Molecular identification and phylogenetic analysis confirmed that isolates 01MB, 04MB, 05MB and 10MB corresponded to Pectobacterium carotovorum subsp. carotovorum; isolates 03MB and 08MB corresponded to Dickeya chrysanthemi; and 02MB and 07MB corresponded to Dickeya paradisiaca and Klebsiella variicola, respectively. It was concluded that rhizome and pseudostem wet rot of banana grown organically in Piura is caused by the bacterial complex formed by P. carotovorum subsp. carotovorum, D. chrysanthemi, D. paradisiaca and K. variicola. This is valuable information for the implementation of appropriate disease management practices in the area.

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Section: A B Csupporting

confidence: 90%

Section: Isolate Pathogenicity Testsupporting

confidence: 89%

Section: A B Csupporting

confidence: 52%

Section: A B Cmentioning

confidence: 99%

Section: A B Cmentioning

confidence: 99%

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CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

Rafael-Rutte¹,

Zavala²,

Maldonado³

et al. 2022

Chil. j. agric. anim. sci.

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NJSE

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Date fruit-Tiger nut drink is a highly nutritious traditional drink. Samples of tiger nut and date fruits were collected from some cities in the three senatorial districts of Delta State: Delta North (Asaba and Agbor); Delta South (Oleh and Warri); and Delta Central (Sapele and Abraka). Phytochemical analysis of lemon grass water extract gave the following results: glycoside, 11.78mg/g; phenols: 121.16mg/g; flavonoids, 43.16mg/g; tannins, 18.47mg/g; and terpenoids, 8.43mg/g; while it was: alkaloids, 31.00mg/g; steroids, 17.00mg/g; saponins, 28.43mg/g; flavonoids, 37.36mg/g; tannins, 14.47mg/g; terpernoids, 12.11mg/g; and phenol, 138mg/g for lemon grass methanolic extracts. The bacteria isolated from the drinks were identified as Beta protoebacterium species, Klebsiella pneumoniae, and Erwinia chrysanthemi strains by biochemical and molecular characterization. Lemon grass methanolic extract inhibited growth of organisms at 60 and 80(mg/ml) concentrations while lemon grass water extract did not inhibit the growth of the organisms at the various concentrations used. A proximate analysis of the Date fruit-Tiger nut drink at intervals of 48hours during treatment with lemon grass methanolic extract showed significant (P<0.05) reductions in carbohydrate (80.64 mg/ml +2.82 to 62.59 mg/ml +4.48), protein (13.46 mg/ml +0.57 to 4.17 mg/ml +0.97) , fat (3.30 mg/ml +0.98 to 1.00 mg/ml +0.40), Crude fibre (14.06 mg/ml +0.32 to 7.40 mg/ml +2.42) and Ash (5.18 mg/ml +0.04 to 5.03 mg/ml+0.05). Moisture contents significantly (P<0.05) increased from (4.2 mg/ml+0.19 to 4.94 mg/ml +0.09). Retarded deterioration of date fruit-tiger nut drink was indicated therefore, methanolic extract can be potentially useful for the preservation of date fruit-tiger nut drink.

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Morphological and Biochemical Properties Useful for Identification of Erwinia chrysanthemi

Cited by 2 publications

References 5 publications

CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

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