Morphological and biochemical relationships in 31 human pituitary adenomas with acromegaly

Trouillas, J.; Girod, C; Lhéritier, M.; Bruno, Claudio; Dubois, M. P.

doi:10.1007/bf00439481

Cited by 55 publications

(8 citation statements)

References 21 publications

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“…In our study we found no relation be¬ tween the percentages of GH-and PRL-cells as de¬ tected by immunocytochemistry and the plasma GH and PRL values, respectively. This discrepancy between immunocytochemical data and plasma GH and PRL levels is in agreement with earlier findings by others (12,25). Data with respect to the frequency of mammosomatotropic cells within pi¬ tuitary adenomas from acromegalic patients are conflicting so far.…”

Section: Resultssupporting

confidence: 83%

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Heterogeneity of pituitary adenoma cell subpopulations from acromegalic patients obtained by Percoll density gradient centrifugation

Hofland

Koetsveld²,

Verleun³

et al. 1989

Acta Endocrinologica

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Pituitary adenoma cells from 6 acromegalic patients were separated on continuous Percoll density gradients according to differences in their density. Two adenomas produced GH only in culture, the other 4 adenomas produced either GH and PRL (one adenoma) or GH and \g=a\-subunit (one adenoma) or GH, PRL and \ g=a\ \ x=r eq-\ subunit (2 adenomas). The cell subpopulations obtained by this technique differed in the amount of hormone production per 105 cells: GH release decreased from the low density fractions to the higher density fractions in 5 of 6 adenomas. Intracellular GH levels completely followed this profile. In the mixed GH/\g=a\-subunitadenomas the \ g=a\ \ x=r eq-\ subunit profile completely paralleled the GH profile, whereas in the mixed GH/PRL adenomas the PRL profile showed a pattern different from that of GH (and \g=a\-subunit). In neither of the adenomas did we find any differences between the subpopulations with respect to the responsiveness of GH, PRL or \g=a\-subunitrelease to GHRH, TRH and the somatostatin analogue SMS 201\ x=req-\ 995. Conclusions: 1. Within pituitary adenomas from acromegalic patients heterogeneity exists with respect to hormone production per cell. 2. The cell subpopulations obtained by density gradient centrifugation are not different in their responsiveness to SMS 201-995, GHRH or TRH. 3. Because GH and \g=a\-subunitrelease by the fractions from the mixed GH/\g=a\-subunit secreting adenomas were completely parallel, further evidence for co-release of GH and \g=a\-subunit by the same tumoural cells is provided.

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Section: Resultssupporting

confidence: 83%

“…This has been demonstrated by several in vitro studies (1)(2)(3)(4)(5)(6) and by morphological techniques (1,(7)(8)(9)(10)(11)(12)(13). However, pituitary adenomas from acro¬ megalic patients are very heterogeneous with re¬ spect to their cellular composition.…”

mentioning

confidence: 87%

Heterogeneity of pituitary adenoma cell subpopulations from acromegalic patients obtained by Percoll density gradient centrifugation

Hofland

Koetsveld²,

Verleun³

et al. 1989

Acta Endocrinologica

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“…suggested that GH and Prl are secreted both by different cells (Corenblum et al 1976) and by the same cells (Trouillas et al 1980) without any corre¬ lation to Prl basal levels.…”

Section: Discussionmentioning

confidence: 98%

Growth hormone releasing factor induces prolactin secretion in acromegalic patients but not in normal subjects

Losa

Schopohl

Müller

et al. 1985

Acta Endocrinologica

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The effect of an iv injection of growth hormone releasing factor (GRF) on Prl secretion in healthy volunteers and patients with active acromegaly was investigated. Thirteen normal subjects received 100 \g=m\g GRF 1-44, and 19 acromegalics received 100 \g=m\g GRF 1-44. Nine normals and 9 patients were given the diluent only and served as placebo control. In healthy volunteers GRF did not affect Prl secretion significantly when compared to placebo, whereas in acromegalics Prl levels after GRF were higher than after placebo. We have divided acromegalics in Prl-responders to GRF (n = 11) and Prl-non-responders (n = 8) using the criterion of strict parallelism between GH and Prl secretion after GRF. In cases with no GH response to GRF a clear increase of Prl levels with the maximum 15\p=n-\30 min after GRF was also regarded as a Prl response. Acromegalic Prl-responders and Prl-non-responders did not significantly differ in age, sex, previous therapy, and basal GH and Prl levels. However, Prl-non-responders had a significantly reduced response of both GH and Prl to TRH (GH: 147.3 \m=+-\16.0 vs 590.1 \m=+-\127.8%; mean \ m=+-\ se; Prl: 159.4 \m=+-\32.6 vs 504.9 \m=+-\ 109.3%). It is concluded that 50 or 100 \g=m\g GRF does not affect Prl secretion in normal subjects. In contrast, in acromegaly GRF leads to Prl secretion in more than half of all patients.

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“…In case of acromegaly, some authors [19] described an inverse correlation of se rum GH levels to granularity ('well' or 'poorly granulated'), whereas others [17,31] could not find such a clear inverse correlation to the granules. (Ultra-)structural signs of ac tivity on the other hand appeared to correlate to serum GH levels [22,31], It might be that these discrepancies are due to the existence of 2 ultrastructurally distinct tumor types exist ing with an equal incidence [15], the densely and the sparsely granulated GH cell adeno mas [16]. Prolactinoma cells, in contrast to GH-producing tumor cells, are almost always sparsely granulated.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Examination of Pituitary Adenomas

et al. 1985

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A comparison was made with the data of 62 cases of pituitary adenoma, evaluated pre- and postoperatively, including as well the results of immunohistochemical hormone examination (also for calcitonin). Prolactin was found in 18 of the 21 adenomas carrying the preoperative diagnosis of prolactinoma, whereas cells containing other hormones (growth hormone, LH, FSH, TSH, ACTH, β-endorphin), were only occasionally present. The growth hormone was strongly positive in the adenoma tissue in 16 of the 17 cases of acromegaly. 5 of these adenomas were accompanied by a marked hyperprolactinemia and also contained many prolactin cells. 6 of the 19 adenomas diagnosed as being ‘inactive’ contained hormone-positive cells, but only a very small number of cells. ACTH was found in 3 of the 4 pituitary adenomas of patients with Cushing’s disease. 2 of these were also positive for β-endorphin. The tissue of 1 gonadotrophic adenoma (with elevated FSH in serum) gave positive results with an anti-LH antiserum. Calcitonin was not found in any adenoma. The preoperative serum prolactin levels did not quantitatively correlate with the percentage of prolactin-positive cells.

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Morphological and biochemical relationships in 31 human pituitary adenomas with acromegaly

Cited by 55 publications

References 21 publications

Heterogeneity of pituitary adenoma cell subpopulations from acromegalic patients obtained by Percoll density gradient centrifugation

Heterogeneity of pituitary adenoma cell subpopulations from acromegalic patients obtained by Percoll density gradient centrifugation

Growth hormone releasing factor induces prolactin secretion in acromegalic patients but not in normal subjects

Immunohistochemical Examination of Pituitary Adenomas

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