Morphological and biochemical traits and mortality in Physalaemus gracilis (Anura: Leptodactylidae) tadpoles exposed to the insecticide chlorpyrifos

Rutkoski, Camila Fátima; Macagnan, Natani; Folador, Alexandre; Skovronski, Vrandrieli Jucieli; Amaral, Aline Monique Blank do; Leitemperger, Jossiele; Costa, Maiara; Hartmann, Paulo Afonso; Müller, Caroline; Loro, Vania Lúcia; Hartmann, Marília Teresinha

doi:10.1016/j.chemosphere.2020.126162

Cited by 36 publications

(15 citation statements)

References 48 publications

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“…The frequency of all ENAs, particularly notched and lobed nuclei, increased considerably at imidacloprid concentrations of 30 µg L −1 and over, demonstrating the extreme genotoxic potential of this pesticide. Nuclear abnormalities are considered to be biomarkers of the impact of pesticides on amphibians 14 , 70 , 71 . As in the present study, notched nuclei were the most common abnormality in Boana pulchella tadpoles exposed to a pirimicarb-based compound 72 .…”

Section: Discussionmentioning

confidence: 99%

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Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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Imidacloprid is a neonicotinoid insecticide used to control agricultural pests around the world. This pesticide can have adverse effects on non-target organisms, especially in aquatic environments. The present study evaluated the toxicity of an imidacloprid-based insecticide in amphibians, using Leptodactylus luctator and Physalaemus cuvieri tadpoles as study models. Spawning of both species were collected within less than 24 h of oviposition from a non-agricultural land at Erechim, Rio Grande do Sul state, Brazil. Survival, swimming activity, body size, morphological malformations, and genotoxic parameters were analyzed at laboratory conditions. A short-term assay was conducted over 168 h (7 days) with five different concentrations of imidacloprid (3–300 µg L−1) being tested. The insecticide did not affect survival, although the tadpoles of both species presented reduced body size, malformed oral and intestine structures, and micronuclei and other erythrocyte nuclear abnormalities following exposure to this imidacloprid-based compound. Exposure also affected swimming activity in L. luctator, which reflected the greater sensitivity of L. luctator to imidacloprid in comparison with P. cuvieri. The swimming activity, body size, and malformations observed in L. luctator and the morphological malformations found in P. cuvieri indicated that even the lowest tested concentration of the insecticide were harmful to amphibians. At concentrations of over 3 μg L−1, P. cuvieri presents a smaller body size, and both species are affected by genotoxic cell damage. This demonstrates that imidacloprid is potentially toxic for the two study species at environmentally relevant concentrations.

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Section: Discussionmentioning

confidence: 99%

“…The dead tadpoles were removed from the containers. Swimming activity was also recorded every 24 h by qualitative observation, based on Rutkoski et al 70 , with modifications. The tadpoles were stimulated gently with a glass rod and the response was recorded.…”

Section: Methodsmentioning

confidence: 99%

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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“…Total ENAs and NN and LN were highly responsive from the concentration of 30 µg L -1 of imidacloprid, which demonstrates the highly genotoxic potential of this pesticide. Although the mechanisms of action of pesticides in nuclear abnormalities have not yet been fully described, these changes are already known to induce the presence of micronuclei and are recently considered biomarkers of the toxic action of pesticides on amphibians (Rutkoski et al 2020;Herek et al 2021;Pavan et al 2021). Among ANEs, notched nucleus was the most sensitive in Boana pulchella tadpoles exposed to pirimicarb-based formulation (Natale et al 2018), as observed in the present study for both species.…”

Section: Discussionmentioning

confidence: 99%

Toxicity and Genotoxicity of Imidacloprid in Tadpoles of Leptodactylus Latrans and Physalaemus Cuvieri (Anura: Leptodactylidae)

Cg¹,

Fa²,

Cf³

et al. 2021

Preprint

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Imidacloprid is a neonicotinoid insecticide widely used worldwide, but which can cause adverse effects on non-target organisms, especially in aquatic environments. This study aimed to evaluate the chronic toxicity of an insecticide-based imidacloprid in amphibians, using Leptodactylus latrans and Physalaemus cuvieri tadpoles. The parameters of survival, swimming activity, body size, damage to body structures and genotoxicity for both species were analyzed; and the ecological risk of this insecticide calculated. Chronic short-term assay was carried out for 168 h (7 days) and five concentrations of imidacloprid, between 3 and 300 µg L-1, were tested. The insecticide did not affect the tadpoles survival tadpoles; however, both species tested showed smaller body size, damage to the mouth and intestine and the induction of micronuclei and other erythrocytes nuclear abnormalities after exposure to imidacloprid-based herbicide. Insecticide exposure affected the swimming activity in L. latrans, which contributes to the greater sensitivity of L. latrans to imidacloprid when compared to P. cuvieri. All parameters analyzed indicated that the insecticide presents an ecological risk for both species at concentrations greater than 3 µg L-1. This demonstrates the genotoxicity of the insecticide imadacloprid, which can contribute to the population decline of L. latrans and P. cuvieri species in natural systems.

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“…In our experiment, the lack of significant effects of chlorpyrifos on survival and development were probably due to the use of relatively low concentrations. The majority of previous studies applied much higher concentrations (Barreto et al, 2020;Bernabò et al, 2011aBernabò et al, , 2011bDe Arcaute et al, 2012;Kendall, 2003, 2002;Rutkoski et al, 2020;Silva et al, 2020;Bidwell, 2008, 2006), although some of these concentrations were referred to as ecologically relevant (Barreto et al, 2020;Bernabò et al, 2011b;Widder and Bidwell, 2008). In these earlier experiments, the applied concentrations were based on surveys from the United States, Canada and South America, where this active ingredient was used in much higher amounts than in Europe e.g.…”

Section: Discussionmentioning

confidence: 99%

Effects of chlorpyrifos on early development and anti-predator behavior of agile frogs

Mikó

Bókony

Újhegyi

et al. 2021

Preprint

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The widespread application of pesticides makes it important to understand the impacts of these chemicals on wildlife populations. Chlorpyrifos is an organophosphate insecticide which can affect the development and behavior of aquatic organisms and may thereby alter predator-prey interactions. To investigate how environmentally relevant, sublethal concentrations of chlorpyrifos affect anti-predator behavior and larval development of the agile frog (Rana dalmatina), we exposed tadpoles to one of three concentrations (0, 0.5 and 5 μg chlorpyrifos / L) either for a brief period of three days (acute exposure) or throughout larval development (chronic exposure). We observed tadpole activity and space use in the presence or absence of chemical cues of predatory fish. We also assessed mortality, time to metamorphosis, mass at metamorphosis, brain morphology and sex ratio. We found that tadpoles chronically exposed to 5 μg/L chlorpyrifos swam distances that were longer by more than 20 % and exhibited body masses at metamorphosis that were lower by ca. 7 % than in control individuals, but the other fitness-related traits remained unaffected. The lower concentration of chlorpyrifos applied chronically, and either one of the acute chlorpyrifos treatments did not influence any measured trait. Our results demonstrate that exposure to chlorpyrifos can induce changes in locomotor activity and may result in lowered body mass of agile frog tadpoles, but only if the insecticide is present chronically at concentrations which are rarely reached in natural waterbodies. Thus, agile frog tadpoles appear to be relatively tolerant to chlorpyrifos, but may nonetheless suffer from its presence in situations of repeated high-dose application.

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Morphological and biochemical traits and mortality in Physalaemus gracilis (Anura: Leptodactylidae) tadpoles exposed to the insecticide chlorpyrifos

Cited by 36 publications

References 48 publications

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Toxicity and Genotoxicity of Imidacloprid in Tadpoles of Leptodactylus Latrans and Physalaemus Cuvieri (Anura: Leptodactylidae)

Effects of chlorpyrifos on early development and anti-predator behavior of agile frogs

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