Morphological and carbogen‐based functional MRI of a chemically induced liver tumor model in mice

Thomas, C.; Chenu, E.; Walczak, Christine; Plessis, Marie-José; Périn, François; Volk, Andreas

doi:10.1002/mrm.10555

Cited by 25 publications

(31 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tumor regions that exhibited positive DR 2 * carbogen followed by positive DR 2 * USPIO are represented on the RGB maps as yellow voxels. Significant increases in tumor R 2 * with carbogen breathing have been previously reported (27,28). As with the cyan voxels, these regions suggest vasculature that remained functional throughout the entire imaging protocol, but the underlying physiology associated with these voxels is challenging to interpret, as positive DR 2 * carbogen does not have a definitive explanation.…”

Section: Cuspio Rgb Image Mapsmentioning

confidence: 59%

Investigating temporal fluctuations in tumor vasculature with combined carbogen and ultrasmall superparamagnetic iron oxide particle (CUSPIO) imaging

Burrell

Walker‐Samuel

Baker

et al. 2011

Magnetic Resonance in Med

View full text Add to dashboard Cite

A combined carbogen ultrasmall superparamagnetic iron oxide (USPIO) imaging protocol was developed and applied in vivo in two murine colorectal tumor xenograft models, HCT116 and SW1222, with established disparate vascular morphology, to investigate whether additional information could be extracted from the combination of two susceptibility MRI biomarkers. Tumors were imaged before and during carbogen breathing and subsequently following intravenous administration of USPIO particles. A novel segmentation method was applied to the image data, from which six categories of R 2 * response were identified, and compared with histological analysis of the vasculature. In particular, a strong association between a negative DR 2 * carbogen followed by positive DR 2 * USPIO with the uptake of the perfusion marker Hoechst 33342 was determined. Regions of tumor tissue where there was a significant DR 2 * carbogen but no significant DR 2 * USPIO were also identified, suggesting these regions became temporally isolated from the vascular supply during the experimental timecourse. These areas correlated with regions of tumor tissue where there was CD31 staining but no Hoechst 33342 uptake. Significantly, different combined carbogen USPIO responses were determined between the two tumor models. Combining DR 2 * carbogen and DR 2 * USPIO with a novel segmentation scheme can facilitate the interpretation of susceptibility contrast MRI data and enable a deeper interrogation of tumor vascular function and architecture. Magn Reson Med 66:227-234,

show abstract

Section: Cuspio Rgb Image Mapsmentioning

confidence: 59%

Investigating temporal fluctuations in tumor vasculature with combined carbogen and ultrasmall superparamagnetic iron oxide particle (CUSPIO) imaging

Burrell

Walker‐Samuel

Baker

et al. 2011

Magnetic Resonance in Med

View full text Add to dashboard Cite

show abstract

“…It has been shown by several groups that breathing of a hyperoxic gas mixture, e.g. carbogen (95% O 2 , 5% CO 2 ), can increase MR signal intensity acquired with a T2*-weighted gradient echo (GRE) sequence in a range of tumours [9][10][11][12][13][14][15][16][17]. This so-called blood oxygen level dependent (BOLD) or flow and oxygenation dependent (FLOOD) effect [14,17] occurs in hypoxic tumour regions, having a functional (i.e.…”

Section: Introductionmentioning

confidence: 99%

Relationship between tumour growth rate and carbogen-based functional MRI for a chemically induced HCC in mice

Thomas

Chenu

Walczak

et al. 2004

MAGMA

View full text Add to dashboard Cite

We previously performed MRI studies of HCC (hepatocellular carcinomas) in mice showing the feasibility of measuring a carbogen effect. In the present study carbogen response of the whole tumour was compared with growth characteristics during longitudinal follow-up. HCC were chemically induced. The imaging protocol at 4.7 T comprised a fast spin-echo sequence for high-resolution screening and measurement of growth curves, and a fast gradient echo sequence allowing an entire T2*w image acquisition per respiratory cycle to perform fMRI under carbogen breathing. A new parameter, T+, the fraction of tumour voxels with increased intensity under carbogen was measured on manually defined ROIs. Twenty-two HCC were followed for 3-10 weeks. Tumours were divided into two groups, "regularly" and "irregularly" growing tumours. A linear correlation between T+ and tumour growth rate was observed only for "regularly" growing HCC. These results suggest a link between tumour growth rates and tumour fractions exhibiting signal increase upon carbogen breathing. They are compatible with observations by others that rapidly growing tumours are more hypoxic than slowly growing ones. Combined measurement of T+ and tumour growth may become a useful noninvasive follow-up approach for assessment and/or management of therapies involving vasculature-targeting and anti-proliferative drugs.

show abstract

“…Endpoints for such investigations are typically pathological, necessitating large animal cohorts to obtain several data points. In this context, the preclinical development and application of noninvasive imaging technologies such as magnetic resonance imaging (MRI) can provide a powerful platform for the assessment of orthotopic tumor formation, invasion, and metastasis, and of the effectiveness of anticancer therapeutics in vivo, while also reducing experimental animal numbers (7)(8)(9).…”

mentioning

confidence: 99%

“…Following administration, USPIO particles are taken up within the reticuloendothelial system (RES) of the liver and spleen (5,11) and passed through the normal pathway of iron metabolism (8,(12)(13)(14). The presence of USPIO particles significantly decreases the MRI transverse relaxation times T 2 * and T 2 , with a subsequent loss in signal intensity, providing negative enhancement and a specific darkening of the normal liver parenchyma (5,12,15).…”

mentioning

confidence: 99%

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Kalber

Waterton

Griffiths

et al. 2008

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose: To characterize longitudinal tumor progression in a murine orthotopic model of liver metastasis using susceptibility contrast magnetic resonance imaging (MRI). Materials and Methods:Nude mice were inoculated intrasplenically with LS174T colorectal carcinoma cells 24 hours postadministration of 2.5 mgFe/kg of the ultrasmall superparamagnetic iron oxide particle preparation feruglose. Contiguous T 2 and T 2 *-weighted multislice MR images were acquired 10, 15, 20, 25, 30, and 35 days postinoculation to longitudinally evaluate metastatic progression. Functional tumor vasculature and hypoxia were histologically evaluated at the final timepoint using Hoechst 33342 uptake, pimonidazole and hematoxylin and eosin staining. A parallel cohort of subcutaneous tumors was included for comparison.Results: All intrasplenically inoculated mice developed liver metastases, evident in both T 2 *-and T 2 -weighted images as high-signal deposits, compared to feruglose-nulled normal liver. Small lesions were detected as early as day 10 and all mice exhibited progressing lesions over 35 days. Liver metastases took longer to establish, but exhibited a similar volume doubling time to the subcutaneously propagated tumors of Ϸ2-3 days. Different functional tumor vascular architectures between the two growth sites were apparent.Conclusion: Susceptibility-contrast MRI using a single dose of feruglose can be used to easily detect and longitudinally monitor orthotopically propagated liver metastases in vivo.

show abstract

Morphological and carbogen‐based functional MRI of a chemically induced liver tumor model in mice

Cited by 25 publications

References 25 publications

Investigating temporal fluctuations in tumor vasculature with combined carbogen and ultrasmall superparamagnetic iron oxide particle (CUSPIO) imaging

Investigating temporal fluctuations in tumor vasculature with combined carbogen and ultrasmall superparamagnetic iron oxide particle (CUSPIO) imaging

Relationship between tumour growth rate and carbogen-based functional MRI for a chemically induced HCC in mice

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Contact Info

Product

Resources

About