Morphological and cultural characteristics of a novel Phellinus linteus KACC93057P

Abstract

Morphological and culture characteristics of the novel Phellinus linteus variant KACC93057P collected in Korea were characterized in this study. The surface of the wasangular, sessile, tough-woody, concentrically zonate, and dark brown in color. Basidiocarppores were circular, with 5-7 pores per mm. The hyphal system was dimitic, and basidiospores were ellipsoid or oval, 4.5-6 × 4-5, exhibiting characteristics typical of P. linteus. The optimum temperature for mycelial growth was 25-30 o C, and optimal pH for growth was 5-7. The mycelial growth rate of P. linteus KACC93057P was faster than that of other P. baumii isolates. On growth medium, KACC93057P formed aerial mycelia with density higher than that of other isolates. The internal transcribed spacer (ITS)-ribosomal DNA sequences were closely related to the sequences P. linteus complex.

Artificial Cultivation Characteristics and Bioactive Effects of Novel Tropicoporus linteus (Syn. Phellinus linteus) Strains HN00K9 and HN6036 in Korea

“…In conclusion, morphological features of HN00K9, ASI26099, and HN6036 were resemble to those of previously reported P. linteus strains [2,[25][26][27][28], but was not to that of P. baumii PBJS.…”

“…Their basidiocarp pores were morphologically circular or angular with similar sizes. In previous study, wild type fruiting body of HN00K9 collected in Korea was hard woody and its basidiocarp pileus surface was concentrically zonate and grooves and had chestnut color, showing typical morphological feature of the reported P. linteus strains [ 27 ]. In its microscopic observation, the surface pore of basidiocarp was golden yellowish color with circular or angular and was 5–7 per mm in size, exhibiting morphological characteristics similar to HN00K9 of this study.…”

“…The styrylpyrone-based polyphenol compounds, davallialactone, hispidin, hypholomine B, Interfungin A, and Inoscavin A were identified in fruiting bodies of HN00K9 by HPLC analysis, but not in mycelium-derived extract, showing many differences in fruiting body and mycelium on polyphenol components [ 35 ]. The phenolic component extracted from fruiting body of HN00K9 was found to have higher antioxidant activity than other mushrooms on analysis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) [ 36 ]. In addition, fruiting body of HN00K9 derived hypholomine B and davallialactone compounds showed proliferative activity without cytotoxicity over 50% LPS (lipopolysaccharide)-activated RAW264.7cell (data not shown).…”

“…In addition, P. linteus strain HN00K9 has been deposited as a patent strain of KACC93057P in Korea Agricultural Culture Collection (KACC, RDA, Korea). The mycelia of the mushroom species were grown on yeast glucose malt (YGM) mediiu [ 27 ] at 25 °C for 20 days.…”

“…A Phellinus sp. HN00K9 was isolated from mulberry tree in Korea and was classified as novel P. linteus based on morphological characteristics of the fruiting body, including spore size, hyphal system, and pore shape and ITS rDNA homology of 98% with those of species [ 27 ]. Interestingly, the P. linteus strains HN00K9 was shown to exhibit rapid mycelial growth rates similar to that of PBJS.…”