Morphological and LSU rDNA sequence variation within the Gonyaulax spinifera-Spiniferites group (Dinophyceae) and proposal of G. elongata comb. nov. and G. membranacea comb. nov.

Ellegaard, Marianne; Daugbjerg, Niels; Rochon, André; Lewis, Jane; Harding, Ian C.

doi:10.2216/i0031-8884-42-2-151.1

Cited by 72 publications

(83 citation statements)

References 12 publications

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“…There was a large sequence divergence observed between multiple isolates in the LSU analyses of G. spinifera, which is consistent with other studies of the LSU region of the Gonyaulax genus (11,20,45,46). While a limited number of LSU sequences were used in this study, distinct ribotypes of toxic G. spinifera (DQ151557 and DQ151558) were revealed.…”

Section: Discussionsupporting

confidence: 89%

Phylogenetic Relationships of Yessotoxin-Producing Dinoflagellates, Based on the Large Subunit and Internal Transcribed Spacer Ribosomal DNA Domains

Howard

Smith

Kudela

2009

Appl Environ Microbiol

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Yessotoxin (YTX) is a globally distributed marine toxin produced by some isolates of the dinoflagellate species Protoceratium reticulatum, Lingulodinium polyedrum, and Gonyaulax spinifera within the order Gonyaulacales. The process of isolating cells and testing each isolate individually for YTX production during toxic blooms are labor intensive, and this impedes our ability to respond quickly to toxic blooms. In this study, we used molecular sequences from the large subunit and internal transcribed spacer genomic regions in the ribosomal operon of known YTX-producing dinoflagellates to determine if genetic differences exist among geographically distinct populations or between toxic and nontoxic isolates within species. In all analyses, all three YTX-producing species fell within the Gonyaulacales order in agreement with morphological taxonomy. Phylogenetic analyses of available rRNA gene sequences indicate that the capacity for YTX production appears to be confined to the order Gonyaulacales. These findings indicate that Gonyaulacoloid dinoflagellate species are the most likely to produce YTX and thus should be prioritized for YTX screening during events. Dinoflagellate species that fall outside of the Gonyaulacales order are unlikely to produce YTX. Although the rRNA operon offers multiple sequence domains to resolve species level diversification within this dinoflagellate order, these domains are not sufficiently variable to provide robust markers for YTX toxicity.

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Section: Discussionsupporting

confidence: 89%

Phylogenetic Relationships of Yessotoxin-Producing Dinoflagellates, Based on the Large Subunit and Internal Transcribed Spacer Ribosomal DNA Domains

Howard

Smith

Kudela

2009

Appl Environ Microbiol

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“…However, using both sequences provided better phylogenetic analyses of C. monotis as has been done with Dinophysis (Edvardsen et al, 2003) and other algae (Huelsenbeck et al, 1996;Buchheim et al, 2001;Harper and Saunders, 2001 Phylogenies inferred from both LSU and SSU neighbor-joining trees were similar, agreeing with the Dinophyceae phylogeny produced by others (e.g. Daugbjerg et al, 2000;Ellegaard et al, 2003;Saldarriaga et al, 2004). In overall, topologies in both of the phylogenetic trees support the morphologybased taxonomic system (Taylor, 1980;Taylor, 1987;Fensome et al, 1993;Steidinger and Tangen, 1997).…”

Section: Discussionsupporting

confidence: 59%

Morphology and rDNA phylogeny of a Mediterranean <em>Coolia monotis</em> (Dinophyceae) strain from Greece

Dolapsakis¹,

Kilpatrick²,

Economou‐Amilli³

et al. 2006

Sci. Mar.

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SUMMARY: Sequences of LSU and SSU ribosomal RNA genes and phylogeny have not been widely investigated for the dinoflagellate Coolia monotis Meunier, and no information is available on the small and large rDNA subunits of Mediterranean strains. A strain isolated from the Thermaikos Gulf in northern Greece was identified as C. monotis-a new record for the Greek algal flora-using thecal morphology by light, epifluorescence and scanning electron microscopy. The small subunit and partial (D1/D2) large subunit sequences were analyzed and compared to other strains of C. monotis and dinoflagellates from various regions. Thecal architecture showed that the Greek strain of C. monotis was phenotypically similar, but not identical, to other strains reported in literature. The partial LSU sequence (700 bp) was found to vary by 113 bp positions (16%) from the C. monotis strain from New Zealand, whereas the SSU (1757 bp) had 15 bp differences (0.85%) from the strain from Norway. Phylogenetic tree construction showed that the Greek strain fell within the Coolia clade and had a close relationship with the families Ostreopsidaceae and Goniodomaceae of the order Gonyaulacales. Preliminary findings suggest the existence of different genotype strains of C. monotis with large intraspecific genetic variability and minimal morphological differentiation (similar phenotypes). Certain ecological and evolutionary implications of these findings are discussed.Keywords: Coolia monotis, Greece, microscopy, rDNA, taxonomy, phylogeny. RESUMEN: MORFOLOGÍA Y FILOGENIA DEL RDNA DE UNA SUBESPECIE MEDITERRÁNEA DE COOLIA MONOTIS (DINOPHYCEAE) DE GRECIA. -Las secuencias de los genes del RNA de las subunidades ribosomales grandes y pequeñas (LSU y SSU, respectivamente) y la filogenia del dinoflagelado Coolia monotis Meunier han sido poco investigadas, y no hay información disponible sobre los genes LSU y SSU de subespecies mediterráneas. Una subespecie aislada del golfo de Thermaikos en el norte de Grecia fue identificada como C. monotis -una nueva aportación a la flora algal griega-por medio de la morfología de la teca observada a través de microscopía óptica, de epifluorescia y electrónica. Las secuencias correspondientes a la subunidad pequeña y a la parte (D1/D2) de la subunidad grande fueron analizadas y comparadas a las de otras subespecies de C. monotis y otras especies de dinoflagelados de diversas regiones. La arquitectura de la teca mostró que la subespecie griega de C. monotis era fenotípicamente similar, pero no idéntica, a otras subespecies registradas en la literatura. Se encontró que la secuencia parcial de la LSU (700 pares de bases o bp) difería de la de C. monotis de Nueva Zelanda en las posiciones de 113 bp (16%), mientras que la SSU (1757 bp) se diferenciaba en 15 bp (0.85%) de la subespecie de Noruega. La construcción del árbol filogenetico demostró que la subespecie griega se situaba dentro de la rama de Coolia y presentaba una relación cercana con las familias Ostreopsidaceae y Goniodomaceae del orden Gonyaulacales. Resultados pre...

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“…fundyense/A. catenella species complex (Supplementary material Table 2, Bolch and de Salas, 2007;Daugbjerg et al, 2000;Ellegaard et al, 2003;Herrera-Sepú lveda et al, 2013;Higman et al, 2001;Kim et al, 2006;Kim and Kim, 2007;Mertens et al, 2012;Orr et al, 2011;Riccardi et al, 2006;Ruiz Sebastiá n and O'Ryan, 2001;Scorzetti et al, 2009;Walsh et al, 1998). A sequence of Prorocentrum minimum was included as outgroup.…”

Section: Molecular Phylogenetic Analysismentioning

confidence: 99%

Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina

et al. 2015

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Morphological and LSU rDNA sequence variation within the Gonyaulax spinifera-Spiniferites group (Dinophyceae) and proposal of G. elongata comb. nov. and G. membranacea comb. nov.

Cited by 72 publications

References 12 publications

Phylogenetic Relationships of Yessotoxin-Producing Dinoflagellates, Based on the Large Subunit and Internal Transcribed Spacer Ribosomal DNA Domains

Phylogenetic Relationships of Yessotoxin-Producing Dinoflagellates, Based on the Large Subunit and Internal Transcribed Spacer Ribosomal DNA Domains

Morphology and rDNA phylogeny of a Mediterranean <em>Coolia monotis</em> (Dinophyceae) strain from Greece

Protoceratium reticulatum (Dinophyceae) in the austral Southwestern Atlantic and the first report on YTX-production in shelf waters of Argentina

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